The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Actin dynamics counteract membrane tension during clathrin-mediated endocytosis
Open accessAbstract
Clathrin-mediated endocytosis is independent of actin dynamics in many circumstances but requires actin polymerization in others. We show that membrane tension determines the actin dependence of clathrin-coat assembly. As found previously, clathrin assembly supports formation of mature coated pits in the absence of actin polymerization on both dorsal and ventral surfaces of non-polarized mammalian cells, and also on basolateral surfaces of polarized cells. Actin engagement is necessary, however, to complete membrane deformation into a coated pit on apical surfaces of polarized cells and, more generally, on the surface of any cell in which the plasma membrane is under tension from osmotic swelling or mechanical stretching. We use these observations to alter actin dependence experimentally and show that resistance of the membrane to propagation of the clathrin lattice determines the distinction between ‘actin dependent and ‘actin independent’. We also find that light-chain-bound Hip1R mediates actin engagement. These data thus provide a unifying explanation for the role of actin dynamics in coated-pit budding.
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Cites
Dynasore, a Cell-Permeable Inhibitor of Dynamin
Macia, E., Ehrlich, M., Massol, R., Boucrot, E., Brunner, C., & Kirchhausen, T. (2006) · Developmental Cell · doi:10.1016/j.devcel.2006.04.002 · Cited by 1,958
Self-citationRohatgi, R., Ma, L., Miki, H., Lopez, M., Kirchhausen, T., Takenawa, T., & Kirschner, M. W. (1999) · Cell · doi:10.1016/s0092-8674(00)80732-1 · Cited by 1,348
Self-citationRational design and characterization of a Rac GTPase-specific small molecule inhibitor
Gao, Y., Dickerson, J. B., Guo, F., Zheng, J., & Zheng, Y. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0307512101 · Cited by 1,091
Endocytosis by random initiation and stabilization of clathrin-coated pits
Ehrlich, M., Boll, W., van Oijen, A., Hariharan, R., Chandran, K., Nibert, M. L., & Kirchhausen, T. (2004) · Cell · doi:10.1016/j.cell.2004.08.017 · Cited by 843
Self-citationA Modular Design for the Clathrin- and Actin-Mediated Endocytosis Machinery
Kaksonen, M., Toret, C. P., & Drubin, D. G. (2005) · Cell · doi:10.1016/j.cell.2005.09.024 · Cited by 726 · cited 1×
“The intensity profile as a function of time was used to characterize the dynamics of each of the AP-2 spots selected according to the following criteria: (1) fluorescent objects where diffraction limited; (2) dynamic, nonarrested objects appeared and disappeared within the time window of the time-series with lifetimes…”
MethodHarnessing actin dynamics for clathrin-mediated endocytosis
Kaksonen, M., Toret, C. P., & Drubin, D. G. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1940 · Cited by 706
Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits
Merrifield, C. J., Feldman, M. E., Wan, L., & Almers, W. (2002) · Nature Cell Biology · doi:10.1038/ncb837 · Cited by 691
Merrifield, C. J., Perrais, D., & Zenisek, D. (2005) · Cell · doi:10.1016/j.cell.2005.03.015 · Cited by 476 · cited 1×
“, but in some circumstances actin and a subset of regulators of short-branch actin assembly, including Arp2/3 (actin-related protein-2/3), cortactin and N-Wasp (also known as Wasl, Wiskott-Aldrich syndrome-like; refs&nbs”
BackgroundGottlieb, T. A., Ivanov, I. E., Adesnik, M., & Sabatini, D. D. (1993) · The Journal of cell biology · doi:10.1083/jcb.120.3.695 · Cited by 440
Membrane Tether Formation from Blebbing Cells
Dai, J., & Sheetz, M. P. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)77168-7 · Cited by 431
Cited by
Mechanisms of clathrin-mediated endocytosis
Kaksonen, M., & Roux, A. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.132 · Cited by 1,698
Dynamin, a membrane-remodelling GTPase
Ferguson, S. M., & De Camilli, P. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3266 · Cited by 992
Cantelmo, A. R., Conradi, L.-C., Brajic, A., Goveia, J., Kalucka, J., Pircher, A., Chaturvedi, P., Hol, J., Thienpont, B., Teuwen, L.-A., et al. (2016) · Cancer Cell · doi:10.1016/j.ccell.2016.10.006 · Cited by 659
Regulation of Clathrin-Mediated Endocytosis
Mettlen, M., Chen, P.-H., Srinivasan, S., Danuser, G., & Schmid, S. L. (2018) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-062917-012644 · Cited by 614
Saheki, Y., & De Camilli, P. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005645 · Cited by 457
Metadata
- Authors
- Boulant, S., Kural, C., Zeeh, J.-C., Ubelmann, F., & Kirchhausen, T.
- Venue
- Nature Cell Biology
- Published
- 2011-08-14
- Volume
- 13
- Issue
- 9
- Pages
- 1124-1131
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W1971060242
- Cited by
- 566
- References
- 46
- FWCI
- 16.47
- Type
- journal article
- Language
- en
- Open access
- green
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