The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Regulation of microtubule motors by tubulin isotypes and post-translational modifications
Open accessAbstract
The ‘tubulin-code’ hypothesis proposes that different tubulin genes or post-translational modifications (PTMs), which mainly confer variation in the carboxy-terminal tail (CTT), result in unique interactions with microtubule-associated proteins for specific cellular functions. However, the inability to isolate distinct and homogeneous tubulin species has hindered biochemical testing of this hypothesis. Here, we have engineered 25 α/β-tubulin heterodimers with distinct CTTs and PTMs and tested their interactions with four different molecular motors using single-molecule assays. Our results show that tubulin isotypes and PTMs can govern motor velocity, processivity and microtubule depolymerization rates, with substantial changes conferred by even single amino acid variation. Revealing the importance and specificity of PTMs, we show that kinesin-1 motility on neuronal β-tubulin (TUBB3) is increased by polyglutamylation and that robust kinesin-2 motility requires detyrosination of α-tubulin. Our results also show that different molecular motors recognize distinctive tubulin ‘signatures’, which supports the premise of the tubulin-code hypothesis.
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Cites
Kinesin superfamily motor proteins and intracellular transport
Hirokawa, N., Noda, Y., Tanaka, Y., & Niwa, S. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2774 · Cited by 1,859
Computer Control of Microscopes Using µManager
Edelstein, A., Amodaj, N., Hoover, K., Vale, R., & Stuurman, N. (2010) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb1420s92 · Cited by 1,670
Self-citationTracking the ends: a dynamic protein network controls the fate of microtubule tips
Akhmanova, A., & Steinmetz, M. O. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2369 · Cited by 1,047
Yildiz, A., Tomishige, M., Vale, R. D., & Selvin, P. R. (2004) · Science · doi:10.1126/science.1093753 · Cited by 965
Self-citationPost-translational regulation of the microtubule cytoskeleton: mechanisms and functions
Janke, C., & Chloë Bulinski, J. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3227 · Cited by 847
Structural Insights into Microtubule Function
Nogales, E. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.277 · Cited by 782
Post-translational modifications regulate microtubule function
Westermann, S., & Weber, K. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1260 · Cited by 717
Single-Molecule Analysis of Dynein Processivity and Stepping Behavior
Reck-Peterson, S. L., Yildiz, A., Carter, A. P., Gennerich, A., Zhang, N., & Vale, R. D. (2006) · Cell · doi:10.1016/j.cell.2006.05.046 · Cited by 635
Self-citationRegulators of the cytoplasmic dynein motor
Kardon, J. R., & Vale, R. D. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2804 · Cited by 602
Self-citationHuman TUBB3 Mutations Perturb Microtubule Dynamics, Kinesin Interactions, and Axon Guidance
Tischfield, M. A., Baris, H. N., Wu, C., Rudolph, G., Van Maldergem, L., He, W., Chan, W.-M., Andrews, C., Demer, J. L., Robertson, R. L., et al. (2010) · Cell · doi:10.1016/j.cell.2009.12.011 · Cited by 595
Cited by
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Robison, P., Caporizzo, M. A., Ahmadzadeh, H., Bogush, A. I., Chen, C. Y., Margulies, K. B., Shenoy, V. B., & Prosser, B. L. (2016) · Science · doi:10.1126/science.aaf0659 · Cited by 338
Metadata
- Authors
- Sirajuddin, M., Rice, L. M., & Vale, R. D.
- Venue
- Nature Cell Biology
- Published
- 2014-03-16
- Volume
- 16
- Issue
- 4
- Pages
- 335-344
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2121302141
- Cited by
- 600
- References
- 64
- FWCI
- 23.80
- Type
- journal article
- Language
- en
- Open access
- green
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