The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Human definitive haemogenic endothelium and arterial vascular endothelium represent distinct lineages
Open accessAbstract
The generation of haematopoietic stem cells (HSCs) from human pluripotent stem cells (hPSCs) will depend on the accurate recapitulation of embryonic haematopoiesis. In the early embryo, HSCs develop from the haemogenic endothelium (HE) and are specified in a Notch-dependent manner through a process named endothelial-to-haematopoietic transition (EHT). As HE is associated with arteries, it is assumed that it represents a subpopulation of arterial vascular endothelium (VE). Here we demonstrate at a clonal level that hPSC-derived HE and VE represent separate lineages. HE is restricted to the CD34+CD73−CD184− fraction of day 8 embryoid bodies and it undergoes a NOTCH-dependent EHT to generate RUNX1C+ cells with multilineage potential. Arterial and venous VE progenitors, in contrast, segregate to the CD34+CD73medCD184+ and CD34+CD73hiCD184− fractions, respectively. Together, these findings identify HE as distinct from VE and provide a platform for defining the signalling pathways that regulate their specification to functional HSCs.
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Cites
Haematopoietic stem cells derive directly from aortic endothelium during development
Bertrand, J. Y., Chi, N. C., Santoso, B., Teng, S., Stainier, D. Y. R., & Traver, D. (2010) · Nature · doi:10.1038/nature08738 · Cited by 1,025
Blood stem cells emerge from aortic endothelium by a novel type of cell transition
Kissa, K., & Herbomel, P. (2010) · Nature · doi:10.1038/nature08761 · Cited by 961
In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium
Boisset, J.-C., van Cappellen, W., Andrieu-Soler, C., Galjart, N., Dzierzak, E., & Robin, C. (2010) · Nature · doi:10.1038/nature08764 · Cited by 917
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Suppression of Notch signalling by the COUP-TFII transcription factor regulates vein identity
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Self-citationNotch1 but Not Notch2 Is Essential for Generating Hematopoietic Stem Cells from Endothelial Cells
Kumano, K., Chiba, S., Kunisato, A., Sata, M., Saito, T., Nakagami-Yamaguchi, E., Yamaguchi, T., Masuda, S., Shimizu, K., Takahashi, T., et al. (2003) · Immunity · doi:10.1016/s1074-7613(03)00117-1 · Cited by 469
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Self-citationKennedy, M., Awong, G., Sturgeon, C. M., Ditadi, A., LaMotte-Mohs, R., Zúñiga-Pflücker, J. C., & Keller, G. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.11.003 · Cited by 400
Self-citationIdentification and targeting of the ROSA26 locus in human embryonic stem cells
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The Varied Roles of Notch in Cancer
Aster, J. C., Pear, W. S., & Blacklow, S. C. (2017) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev-pathol-052016-100127 · Cited by 710
Olgasi, C., Cucci, A., & Follenzi, A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21176215 · Cited by 100
Dege, C., Fegan, K. H., Creamer, J. P., Berrien-Elliott, M. M., Luff, S. A., Kim, D., Wagner, J. A., Kingsley, P. D., McGrath, K. E., Fehniger, T. A., et al. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.02.016 · Cited by 93
Self-citationZhang, T., Huang, K., Zhu, Y., Wang, T., Shan, Y., Long, B., Li, Y., Chen, Q., Wang, P., Zhao, S., et al. (2019) · Journal of Biological Chemistry · doi:10.1074/jbc.ra119.009757 · Cited by 47
Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium
Ghersi, J. J., Baldissera, G., Hintzen, J., Luff, S. A., Cheng, S., Xia, I. F., Sturgeon, C. M., & Nicoli, S. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01187-9 · Cited by 32
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Metadata
- Authors
- Ditadi, A., Sturgeon, C. M., Tober, J., Awong, G., Kennedy, M., Yzaguirre, A. D., Azzola, L., Ng, E. S., Stanley, E. G., French, D. L., Cheng, X., Gadue, P., Speck, N. A., Elefanty, A. G., & Keller, G.
- Venue
- Nature Cell Biology
- Published
- 2015-04-27
- Volume
- 17
- Issue
- 5
- Pages
- 580-591
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2053831749
- Cited by
- 320
- References
- 44
- FWCI
- 21.26
- Type
- journal article
- Language
- en
- Open access
- green
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- Ditadi, A., Sturgeon, C. M., Tober, J., Awong, G., Kennedy, M., Yzaguirre, A. D., Azzola, L., Ng, E. S., Stanley, E. G., French, D. L., Cheng, X., Gadue, P., Speck, N. A., Elefanty, A. G., & Keller, G. (2015). Human definitive haemogenic endothelium and arterial vascular endothelium represent distinct lineages. Nature Cell Biology. https://doi.org/10.1038/ncb3161
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