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Cited by 100Pluripotent Stem Cells ResearchTissue Engineering and Regenerative MedicineLiver physiology and pathology

iPSC-Derived Liver Organoids: A Journey from Drug Screening, to Disease Modeling, Arriving to Regenerative Medicine

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Olgasi, Cristina, Cucci, Alessia, Follenzi, Antonia·2020·International Journal of Molecular Sciences
DOI 10.3390/ijms21176215PMC PMC7503935
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Abstract

Liver transplantation is the most common treatment for patients suffering from liver failure that is caused by congenital diseases, infectious agents, and environmental factors. Despite a high rate of patient survival following transplantation, organ availability remains the key limiting factor. As such, research has focused on the transplantation of different cell types that are capable of repopulating and restoring liver function. The best cellular mix capable of engrafting and proliferating over the long-term, as well as the optimal immunosuppression regimens, remain to be clearly well-defined. Hence, alternative strategies in the field of regenerative medicine have been explored. Since the discovery of induced pluripotent stem cells (iPSC) that have the potential of differentiating into a broad spectrum of cell types, many studies have reported the achievement of iPSCs differentiation into liver cells, such as hepatocytes, cholangiocytes, endothelial cells, and Kupffer cells. In parallel, an increasing interest in the study of self-assemble or matrix-guided three-dimensional (3D) organoids have paved the way for functional bioartificial livers. In this review, we will focus on the recent breakthroughs in the development of iPSCs-based liver organoids and the major drawbacks and challenges that need to be overcome for the development of future applications.

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Cited by

Sorted by citations

Authors
Olgasi, C., Cucci, A., & Follenzi, A.
Affiliations
Università degli Studi del Piemonte Orientale “Amedeo Avogadro” (IT)
Corresponding author
Follenzi, Antonia
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2020-08-27
Volume
21
Issue
17
Pages
6215
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms21176215
PMC
PMC7503935
OpenAlex
W3082905147
Cited by
100
References
28
Citations by year
2026: 8 · 2025: 20 · 2024: 23 · 2023: 23 · 2022: 15 · 2021: 10 · 2020: 1
FWCI
1.17
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Horizon 2020 Framework Programme (667421)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
induced pluripotent stem cells, liver regeneration, bioartificial liver, hepatocyte differentiation, cholangiocytes, Kupffer cells, liver transplantation, drug screening, disease modeling, regenerative medicine
MeSH terms
Cell Differentiation, Drug Evaluation, Preclinical, Humans, Liver, Models, Biological, Organ Culture Techniques, Organoids, Liver Transplantation, Regenerative Medicine, Induced Pluripotent Stem Cells

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