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Subcellular metal imaging identifies dynamic sites of Cu accumulation in Chlamydomonas
Open accessAbstract
We identified a Cu accumulating structure with a dynamic role in intracellular Cu homeostasis. During Zn limitation, Chlamydomonas reinhardtii hyperaccumulated Cu, dependent on the nutritional Cu sensor CRR1, but was functionally Cu-deficient. Visualization of intracellular Cu revealed major Cu accumulation sites coincident with electron-dense structures that stained positive for low pH and polyphosphate, suggesting that they are lysosome-related organelles. NanoSIMS showed colocalization of Ca and Cu, and X-ray absorption spectroscopy (XAS) was consistent with Cu+ accumulation in an ordered structure. Zn resupply restored Cu homeostasis concomitant with reduced abundance of these structures. Cu isotope labeling demonstrated that sequestered Cu+ became bio-available for the synthesis of plastocyanin, and transcriptome profiling indicated that mobilized Cu became visible to CRR1. Cu trafficking to intracellular accumulation sites may be a strategy for preventing protein mis-metallation during Zn deficiency and enabling efficient cuproprotein (re)-metallation upon Zn resupply.
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Kim, D., Pertea, G., Trapnell, C., Pimentel, H., Kelley, R., & Salzberg, S. L. (2013) · Genome Biology · doi:10.1186/gb-2013-14-4-r36 · Cited by 12,668
637. The stability of transition-metal complexes
Irving, H., & Williams, R. J. P. (1953) · Journal of the Chemical Society (Resumed) · doi:10.1039/jr9530003192 · Cited by 2,116
Metalloproteins and metal sensing
Waldron, K. J., Rutherford, J. C., Ford, D., & Robinson, N. J. (2009) · Nature · doi:10.1038/nature08300 · Cited by 1,269
Metal ions in biological catalysis: from enzyme databases to general principles
Andreini, C., Bertini, I., Cavallaro, G., Holliday, G. L., & Thornton, J. M. (2008) · JBIC Journal of Biological Inorganic Chemistry · doi:10.1007/s00775-008-0404-5 · Cited by 1,150
Zinc transporters and the cellular trafficking of zinc
Eide, D. J. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2006.03.005 · Cited by 874
Acidocalcisomes ? conserved from bacteria to man
Docampo, R., de Souza, W., Miranda, K., Rohloff, P., & Moreno, S. N. J. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1097 · Cited by 468
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Self-citationSynthetic fluorescent probes for studying copper in biological systems
Cotruvo, Jr., Aron, A. T., Ramos-Torres, K. M., & Chang, C. J. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00346b · Cited by 558
Self-citationCotruvo, J. A. (2019) · ACS Central Science · doi:10.1021/acscentsci.9b00642 · Cited by 317
Aron, A. T., Ramos-Torres, K. M., Cotruvo, J. A., & Chang, C. J. (2015) · Accounts of Chemical Research · doi:10.1021/acs.accounts.5b00221 · Cited by 280
Self-citationGrover, K., Koblova, A., Pezacki, A. T., Chang, C. J., & New, E. J. (2024) · Chemical Reviews · doi:10.1021/acs.chemrev.3c00819 · Cited by 129
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Metadata
- Authors
- Hong-Hermesdorf, A., Miethke, M., Gallaher, S. D., Kropat, J., Dodani, S. C., Chan, J., Barupala, D., Domaille, D. W., Shirasaki, D. I., Loo, J. A., Weber, P. K., Pett-Ridge, J., Stemmler, T. L., Chang, C. J., & Merchant, S. S.
- Venue
- Nature Chemical Biology
- Published
- 2014-10-26
- Volume
- 10
- Issue
- 12
- Pages
- 1034-1042
- ISSN
- 1552-4450 (ISSN-L)
- 1552-4469
- OpenAlex
- W2030572315
- Cited by
- 165
- References
- 6
- FWCI
- 19.46
- Type
- journal article
- Language
- en
- Open access
- green
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- Hong-Hermesdorf, A., Miethke, M., Gallaher, S. D., Kropat, J., Dodani, S. C., Chan, J., Barupala, D., Domaille, D. W., Shirasaki, D. I., Loo, J. A., Weber, P. K., Pett-Ridge, J., Stemmler, T. L., Chang, C. J., & Merchant, S. S. (2014). Subcellular metal imaging identifies dynamic sites of Cu accumulation in Chlamydomonas. Nature Chemical Biology. https://doi.org/10.1038/nchembio.1662
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