The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Eukaryotic DNA polymerases require an iron-sulfur cluster for the formation of active complexes
Open accessAbstract
The eukaryotic replicative DNA polymerases (Pol α, δ, and ε), and the major DNA mutagenesis enzyme Pol ζ contain two conserved cysteine-rich metal-binding motifs (CysA and CysB) in the C-terminal domain (CTD) of their catalytic subunits. Here, we demonstrate by in vivo and in vitro approaches the presence of an essential [4Fe-4S] cluster in the CysB motif of all four yeast B-family DNA polymerases. Loss of the [4Fe-4S] cofactor by cysteine ligand mutagenesis in Pol3 destabilized the CTD and abrogated interaction with the Pol31-Pol32 subunits. Reciprocally, overexpression of accessory subunits increased the amount of CTD-bound Fe-S cluster. This implies an important physiological role of the Fe-S cluster in polymerase complex stabilization. Further, we demonstrate that the Zn-binding CysA motif is required for PCNA-mediated Pol δ processivity. Together, our findings show that the function of eukaryotic replicative DNA polymerases crucially depends on different metallocenters for accessory subunit recruitment and for replisome stability.
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Cites
Function and biogenesis of iron-sulphur proteins
Lill, R. (2009) · Nature · doi:10.1038/nature08301 · Cited by 1,167
Self-citationEUKARYOTIC TRANSLESION SYNTHESIS DNA POLYMERASES: Specificity of Structure and Function
Prakash, S., Johnson, R. E., & Prakash, L. (2005) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.74.082803.133250 · Cited by 1,000
Structural classification of zinc fingers: SURVEY AND SUMMARY
Krishna, S. S. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg161 · Cited by 917
The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins
Kispal, G., Csere, P., Prohl, C., & Lill, R. (1999) · The EMBO Journal · doi:10.1093/emboj/18.14.3981 · Cited by 700
Self-citationThymine-Thymine Dimer Bypass by Yeast DNA Polymerase ζ
Nelson, J. R., Lawrence, C. W., & Hinkle, D. C. (1996) · Science · doi:10.1126/science.272.5268.1646 · Cited by 654
Recombinant replication protein A: expression, complex formation, and functional characterization
Henricksen, L. A., Umbricht, C. B., & Wold, M. S. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)78100-9 · Cited by 597
Yeast DNA Polymerase ε Participates in Leading-Strand DNA Replication
Pursell, Z. F., Isoz, I., Lundström, E.-B., Johansson, E., & Kunkel, T. A. (2007) · Science · doi:10.1126/science.1144067 · Cited by 563
Division of Labor at the Eukaryotic Replication Fork
Nick McElhinny, S. A., Gordenin, D. A., Stith, C. M., Burgers, P. M. J., & Kunkel, T. A. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.02.022 · Cited by 502
Self-citationMitochondrial Dysfunction Leads to Nuclear Genome Instability via an Iron-Sulfur Cluster Defect
Veatch, J. R., McMurray, M. A., Nelson, Z. W., & Gottschling, D. E. (2009) · Cell · doi:10.1016/j.cell.2009.04.014 · Cited by 425
Eukaryotic DNA Polymerases: Proposal for a Revised Nomenclature
Burgers, P. M. J., Koonin, E. V., Bruford, E., Blanco, L., Burtis, K. C., Christman, M. F., Copeland, W. C., Friedberg, E. C., Hanaoka, F., Hinkle, D. C., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.r100056200 · Cited by 337
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Cited by
Iron and cancer: more ore to be mined
Torti, S. V., & Torti, F. M. (2013) · Nature Reviews Cancer · doi:10.1038/nrc3495 · Cited by 1,621
Mechanisms of Mitochondrial Iron-Sulfur Protein Biogenesis
Lill, R., & Freibert, S.-A. (2020) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-013118-111540 · Cited by 453
Self-citationMitochondria and Iron: current questions
Paul, B. T., Manz, D. H., Torti, F. M., & Torti, S. V. (2017) · Expert Review of Hematology · doi:10.1080/17474086.2016.1268047 · Cited by 423
Essential functions of iron-requiring proteins in DNA replication, repair and cell cycle control
Zhang, C. (2014) · Protein & Cell · doi:10.1007/s13238-014-0083-7 · Cited by 297
MMS19 Assembles Iron-Sulfur Proteins Required for DNA Metabolism and Genomic Integrity
Stehling, O., Vashisht, A. A., Mascarenhas, J., Jonsson, Z. O., Sharma, T., Netz, D. J. A., Pierik, A. J., Wohlschlegel, J. A., & Lill, R. (2012) · Science · doi:10.1126/science.1219723 · Cited by 288
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Metadata
- Authors
- Netz, D. J. A., Stith, C. M., Stümpfig, M., Köpf, G., Vogel, D., Genau, H. M., Stodola, J. L., Lill, R., Burgers, P. M. J., & Pierik, A. J.
- Venue
- Nature Chemical Biology
- Published
- 2011-11-27
- Volume
- 8
- Issue
- 1
- Pages
- 125-132
- ISSN
- 1552-4450 (ISSN-L)
- 1552-4469
- OpenAlex
- W2120269671
- Cited by
- 384
- References
- 48
- FWCI
- 9.98
- Type
- journal article
- Language
- en
- Open access
- green
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- Netz, D. J. A., Stith, C. M., Stümpfig, M., Köpf, G., Vogel, D., Genau, H. M., Stodola, J. L., Lill, R., Burgers, P. M. J., & Pierik, A. J. (2012). Eukaryotic DNA polymerases require an iron-sulfur cluster for the formation of active complexes. Nature Chemical Biology. https://doi.org/10.1038/nchembio.721
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