The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Chromatin topology is coupled to Polycomb group protein subnuclear organization
Open accessAbstract
The genomes of metazoa are organized at multiple scales. Many proteins that regulate genome architecture, including Polycomb group (PcG) proteins, form subnuclear structures. Deciphering mechanistic links between protein organization and chromatin architecture requires precise description and mechanistic perturbations of both. Using super-resolution microscopy, here we show that PcG proteins are organized into hundreds of nanoscale protein clusters. We manipulated PcG clusters by disrupting the polymerization activity of the sterile alpha motif (SAM) of the PcG protein Polyhomeotic (Ph) or by increasing Ph levels. Ph with mutant SAM disrupts clustering of endogenous PcG complexes and chromatin interactions while elevating Ph level increases cluster number and chromatin interactions. These effects can be captured by molecular simulations based on a previously described chromatin polymer model. Both perturbations also alter gene expression. Organization of PcG proteins into small, abundant clusters on chromatin through Ph SAM polymerization activity may shape genome architecture through chromatin interactions.
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Simonis, M., Klous, P., Splinter, E., Moshkin, Y., Willemsen, R., de Wit, E., van Steensel, B., & de Laat, W. (2006) · Nature Genetics · doi:10.1038/ng1896 · Cited by 1,386
Carmesin, I., & Kremer, K. (1988) · Macromolecules · doi:10.1021/ma00187a030 · Cited by 1,267
Complexity of chromatin folding is captured by the strings and binders switch model
Barbieri, M., Chotalia, M., Fraser, J., Lavitas, L.-M., Dostie, J., Pombo, A., & Nicodemi, M. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1204799109 · Cited by 570
Functional implications of genome topology
Cavalli, G., & Misteli, T. (2013) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2474 · Cited by 415
Polycomb repressive complex PRC1 spatially constrains the mouse embryonic stem cell genome
Schoenfelder, S., Sugar, R., Dimond, A., Javierre, B.-M., Armstrong, H., Mifsud, B., Dimitrova, E., Matheson, L., Tavares-Cadete, F., Furlan-Magaril, M., et al. (2015) · Nature Genetics · doi:10.1038/ng.3393 · Cited by 398
Simon, J., Peifer, M., Bender, W., & O'Connor, M. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb07615.x · Cited by 159
Cited by
Genome Regulation by Polycomb and Trithorax: 70 Years and Counting
Schuettengruber, B., Bourbon, H.-M., Di Croce, L., & Cavalli, G. (2017) · Cell · doi:10.1016/j.cell.2017.08.002 · Cited by 1,094
Single-molecule localization microscopy
Lelek, M., Gyparaki, M. T., Beliu, G., Schueder, F., Griffié, J., Manley, S., Jungmann, R., Sauer, M., Lakadamyali, M., & Zimmer, C. (2021) · Nature Reviews Methods Primers · doi:10.1038/s43586-021-00038-x · Cited by 954
The role of 3D genome organization in development and cell differentiation
Zheng, H., & Xie, W. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0132-4 · Cited by 719
The Many Roles of BAF (mSWI/SNF) and PBAF Complexes in Cancer
Hodges, C., Kirkland, J. G., & Crabtree, G. R. (2016) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a026930 · Cited by 414
Kundu, S., Ji, F., Sunwoo, H., Jain, G., Lee, J. T., Sadreyev, R. I., Dekker, J., & Kingston, R. E. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.01.009 · Cited by 333
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Metadata
- Authors
- Wani, A. H., Boettiger, A. N., Schorderet, P., Ergun, A., Münger, C., Sadreyev, R. I., Zhuang, X., Kingston, R. E., & Francis, N. J.
- Venue
- Nature Communications
- Published
- 2016-01-13
- Volume
- 7
- Issue
- 1
- Pages
- 10291-10291
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2233967916
- Cited by
- 198
- References
- 6
- FWCI
- 11.74
- Type
- journal article
- Language
- en
- Open access
- gold
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- Wani, A. H., Boettiger, A. N., Schorderet, P., Ergun, A., Münger, C., Sadreyev, R. I., Zhuang, X., Kingston, R. E., & Francis, N. J. (2016). Chromatin topology is coupled to Polycomb group protein subnuclear organization. Nature Communications. https://doi.org/10.1038/ncomms10291
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