The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Octanol-assisted liposome assembly on chip
Open accessAbstract
Liposomes are versatile supramolecular assemblies widely used in basic and applied sciences. Here we present a novel microfluidics-based method, octanol-assisted liposome assembly (OLA), to form monodisperse, cell-sized (5–20 μm), unilamellar liposomes with excellent encapsulation efficiency. Akin to bubble blowing, an inner aqueous phase and a surrounding lipid-carrying 1-octanol phase is pinched off by outer fluid streams. Such hydrodynamic flow focusing results in double-emulsion droplets that spontaneously develop a side-connected 1-octanol pocket. Owing to interfacial energy minimization, the pocket splits off to yield fully assembled solvent-free liposomes within minutes. This solves the long-standing fundamental problem of prolonged presence of residual oil in the liposome bilayer. We demonstrate the unilamellarity of liposomes with functional α-haemolysin protein pores in the membrane and validate the biocompatibility by inner leaflet localization of bacterial divisome proteins (FtsZ and ZipA). OLA offers a versatile platform for future analytical tools, delivery systems, nanoreactors and synthetic cells.
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Cites
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Liposomes: Technologies and Analytical Applications
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Cited by
Microfluidic approaches for producing lipid-based nanoparticles for drug delivery applications
Piunti, C., & Cimetta, E. (2023) · Biophysics Reviews · doi:10.1063/5.0150345 · Cited by 37
Metadata
- Authors
- Deshpande, S., Caspi, Y., Meijering, A. E. C., & Dekker, C.
- Venue
- Nature Communications
- Published
- 2016-01-22
- Volume
- 7
- Issue
- 1
- Pages
- 10447-10447
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2300212614
- Cited by
- 363
- References
- 34
- FWCI
- 14.44
- Type
- journal article
- Language
- en
- Open access
- gold
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