The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Octanol-assisted liposome assembly on chip
Open accessAbstract
Liposomes are versatile supramolecular assemblies widely used in basic and applied sciences. Here we present a novel microfluidics-based method, octanol-assisted liposome assembly (OLA), to form monodisperse, cell-sized (5–20 μm), unilamellar liposomes with excellent encapsulation efficiency. Akin to bubble blowing, an inner aqueous phase and a surrounding lipid-carrying 1-octanol phase is pinched off by outer fluid streams. Such hydrodynamic flow focusing results in double-emulsion droplets that spontaneously develop a side-connected 1-octanol pocket. Owing to interfacial energy minimization, the pocket splits off to yield fully assembled solvent-free liposomes within minutes. This solves the long-standing fundamental problem of prolonged presence of residual oil in the liposome bilayer. We demonstrate the unilamellarity of liposomes with functional α-haemolysin protein pores in the membrane and validate the biocompatibility by inner leaflet localization of bacterial divisome proteins (FtsZ and ZipA). OLA offers a versatile platform for future analytical tools, delivery systems, nanoreactors and synthetic cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Liposomal drug delivery systems: From concept to clinical applications
Allen, T. M., & Cullis, P. R. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.09.037 · Cited by 4,839
Structure of Staphylococcal α-Hemolysin, a Heptameric Transmembrane Pore
Song, L., Hobaugh, M. R., Shustak, C., Cheley, S., Bayley, H., & Gouaux, J. E. (1996) · Science · doi:10.1126/science.274.5294.1859 · Cited by 2,377
Computer Control of Microscopes Using µManager
Edelstein, A., Amodaj, N., Hoover, K., Vale, R., & Stuurman, N. (2010) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb1420s92 · Cited by 1,670
Angelova, M. I., & Dimitrov, D. S. (1986) · Faraday Discussions of the Chemical Society · doi:10.1039/dc9868100303 · Cited by 1,372
A vesicle bioreactor as a step toward an artificial cell assembly
Noireaux, V., & Libchaber, A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408236101 · Cited by 1,152
Preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes
Olson, F., Hunt, C. A., Szoka, F. C., Vail, W. J., & Papahadjopoulos, D. (1979) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(79)90085-3 · Cited by 1,126
Lipowsky, R. (1991) · Nature · doi:10.1038/349475a0 · Cited by 978
Formation and properties of thin‐walled phospholipid vesicles
Reeves, J. P., & Dowben, R. M. (1969) · Journal of Cellular Physiology · doi:10.1002/jcp.1040730108 · Cited by 656
Liposomes: Technologies and Analytical Applications
Jesorka, A., & Orwar, O. (2008) · Annual Review of Analytical Chemistry · doi:10.1146/annurev.anchem.1.031207.112747 · Cited by 569
Reconstitution of Contractile FtsZ Rings in Liposomes
Osawa, M., Anderson, D. E., & Erickson, H. P. (2008) · Science · doi:10.1126/science.1154520 · Cited by 514
GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission
Roux, A., Uyhazi, K., Frost, A., & De Camilli, P. (2006) · Nature · doi:10.1038/nature04718 · Cited by 490
Engineering asymmetric vesicles
Pautot, S., Frisken, B. J., & Weitz, D. A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1931005100 · Cited by 470
Cascades of Transient Pores in Giant Vesicles: Line Tension and Transport
Karatekin, E., Sandre, O., Guitouni, H., Borghi, N., Puech, P.-H., & Brochard-Wyart, F. (2003) · Biophysical Journal · doi:10.1016/s0006-3495(03)74981-9 · Cited by 416
Microfluidic methods for forming liposomes
van Swaay, D., & deMello, A. (2013) · Lab on a Chip · doi:10.1039/c2lc41121k · Cited by 379
Double Emulsion Templated Monodisperse Phospholipid Vesicles
Shum, H. C., Lee, D., Yoon, I., Kodger, T., & Weitz, D. A. (2008) · Langmuir · doi:10.1021/la801833a · Cited by 362
Jansen, H. V., de Boer, M. J., Unnikrishnan, S., Louwerse, M. C., & Elwenspoek, M. C. (2009) · Journal of Micromechanics and Microengineering · doi:10.1088/0960-1317/19/3/033001 · Cited by 318
Estimation of interfacial tension between organic liquids and water
Demond, A. H., & Lindner, A. S. (1993) · Environmental Science & Technology · doi:10.1021/es00048a004 · Cited by 290
Unilamellar vesicle formation and encapsulation by microfluidic jetting
Stachowiak, J. C., Richmond, D. L., Li, T. H., Liu, A. P., Parekh, S. H., & Fletcher, D. A. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0710875105 · Cited by 290
Actin-binding proteins sensitively mediate F-actin bundle stiffness
Claessens, M. M. A. E., Bathe, M., Frey, E., & Bausch, A. R. (2006) · Nature Materials · doi:10.1038/nmat1718 · Cited by 266
Stepwise Synthesis of Giant Unilamellar Vesicles on a Microfluidic Assembly Line
Matosevic, S., & Paegel, B. M. (2011) · Journal of the American Chemical Society · doi:10.1021/ja109137s · Cited by 205
Fenz, S. F., & Sengupta, K. (2012) · Integrative Biology · doi:10.1039/c2ib00188h · Cited by 196
Arriaga, L. R., Datta, S. S., Kim, S., Amstad, E., Kodger, T. E., Monroy, F., & Weitz, D. A. (2014) · Small · doi:10.1002/smll.201301904 · Cited by 191
Formation of Giant Lipid Vesiclelike Compartments from a Planar Lipid Membrane by a Pulsed Jet Flow
Funakoshi, K., Suzuki, H., & Takeuchi, S. (2007) · Journal of the American Chemical Society · doi:10.1021/ja074029f · Cited by 184
Microfluidic Formation of Monodisperse, Cell‐Sized, and Unilamellar Vesicles
Ota, S., Yoshizawa, S., & Takeuchi, S. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200902182 · Cited by 176
Layer-by-layer cell membrane assembly
Matosevic, S., & Paegel, B. M. (2013) · Nature Chemistry · doi:10.1038/nchem.1765 · Cited by 158
Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics
Teh, S., Khnouf, R., Fan, H., & Lee, A. P. (2011) · Biomicrofluidics · doi:10.1063/1.3665221 · Cited by 156
Genetic Analysis of the Escherichia coli FtsZ·ZipA Interaction in the Yeast Two-hybrid System
Haney, S. A., Glasfeld, E., Hale, C. A., Keeney, D. R., He, Z., & de Boer, P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m009810200 · Cited by 128
The order of the ring: assembly of Escherichia coli cell division components
Vicente, M., & Rico, A. I. (2006) · Molecular Microbiology · doi:10.1111/j.1365-2958.2006.05233.x · Cited by 124
A bacterial dynamin‐like protein mediating nucleotide‐independent membrane fusion
Bürmann, F., Ebert, N., van Baarle, S., & Bramkamp, M. (2011) · Molecular Microbiology · doi:10.1111/j.1365-2958.2011.07523.x · Cited by 85
Bacterial Division Proteins FtsZ and ZipA Induce Vesicle Shrinkage and Cell Membrane Invagination
Cabré, E. J., Sánchez-Gorostiaga, A., Carrara, P., Ropero, N., Casanova, M., Palacios, P., Stano, P., Jiménez, M., Rivas, G., & Vicente, M. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.491688 · Cited by 80
Divided we stand: splitting synthetic cells for their proliferation
Caspi, Y., & Dekker, C. (2014) · Systems and Synthetic Biology · doi:10.1007/s11693-014-9145-7 · Cited by 55
Self-citationA label-free microfluidic assay to quantitatively study antibiotic diffusion through lipid membranes
Cama, J., Chimerel, C., Pagliara, S., Javer, A., & Keyser, U. F. (2014) · Lab on a Chip · doi:10.1039/c4lc00217b · Cited by 42
α-Hemolysin membrane pore density measured on liposomes
Lemiere, J. P. C., Guevorkian, K., Campillo, C. C., Sykes, C., & Betz, T. (2013) · Soft Matter · doi:10.1039/c3sm27812c · Cited by 23
Mizuno, M., Toyota, T., Konishi, M., Kageyama, Y., Yamada, M., & Seki, M. (2015) · Langmuir · doi:10.1021/acs.langmuir.5b00043 · Cited by 22
Cited by
Recent advancements in liposome technology
Filipczak, N., Pan, J., Yalamarty, S. S. K., & Torchilin, V. P. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.06.022 · Cited by 592
Microfluidic fabrication of microparticles for biomedical applications
Li, W., Zhang, L., Ge, X., Xu, B., Zhang, W., Qu, L., Choi, C.-H., Xu, J., Zhang, A., Lee, H., et al. (2018) · Chemical Society Reviews · doi:10.1039/c7cs00263g · Cited by 585
A comprehensive review on recent preparation techniques of liposomes
Has, C., & Sunthar, P. (2020) · Journal of Liposome Research · doi:10.1080/08982104.2019.1668010 · Cited by 357
Maeki, M., Kimura, N., Sato, Y., Harashima, H., & Tokeshi, M. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.03.008 · Cited by 319
Mastering Complexity: Towards Bottom-up Construction of Multifunctional Eukaryotic Synthetic Cells
Göpfrich, K., Platzman, I., & Spatz, J. P. (2018) · Trends in Biotechnology · doi:10.1016/j.tibtech.2018.03.008 · Cited by 267
Metadata
- Authors
- Deshpande, S., Caspi, Y., Meijering, A. E. C., & Dekker, C.
- Venue
- Nature Communications
- Published
- 2016-01-22
- Volume
- 7
- Issue
- 1
- Pages
- 10447-10447
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2300212614
- Cited by
- 363
- References
- 34
- FWCI
- 14.44
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
