The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
An optimized small molecule inhibitor cocktail supports long-term maintenance of human embryonic stem cells
Open accessAbstract
A major challenge in stem-cell mediated regenerative medicine is the development of defined culture systems for the maintenance of clinical-grade human embryonic stem (hES) cells. Using a feedback system control scheme, we identified a unique combination of three small molecule inhibitors that enables the maintenance of hES cells on a fibronectin-coated surface through single cell passaging. After 20 passages, the undifferentiated state of the hES cells was confirmed by OCT4, SSEA4 and NANOG expressions, while their pluripotent potential and genetic integrity were demonstrated by teratoma formation and normal karyotype, respectively. Our study attests to the power of feedback system control scheme in quickly pinpointing optimal conditions for desired biological activities and provides a chemically defined, scalable and single cell passaging culture system for hES cells.
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Cites
Storn, R., & Price, K. (1997) · Journal of Global Optimization · doi:10.1023/a:1008202821328 · Cited by 29,569
The ground state of embryonic stem cell self-renewal
Ying, Q.-L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., & Smith, A. (2008) · Nature · doi:10.1038/nature06968 · Cited by 3,442
A ROCK inhibitor permits survival of dissociated human embryonic stem cells
Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J. B., Nishikawa, S., Nishikawa, S.-I., Muguruma, K., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1310 · Cited by 2,055
Derivation of human embryonic stem cells in defined conditions
Ludwig, T. E., Levenstein, M. E., Jones, J. M., Berggren, W. T., Mitchen, E. R., Frane, J. L., Crandall, L. J., Daigh, C. A., Conard, K. R., Piekarczyk, M. S., et al. (2006) · Nature Biotechnology · doi:10.1038/nbt1177 · Cited by 1,114
Hydrophobic surfaces for enhanced differentiation of embryonic stem cell-derived embryoid bodies
Valamehr, B., Jonas, S. J., Polleux, J., Qiao, R., Guo, S., Gschweng, E. H., Stiles, B., Kam, K., Luo, T.-J. M., Witte, O. N., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0807235105 · Cited by 155
Self-citation
Cited by
Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., et al. (2012) · Nature Communications · doi:10.1038/ncomms2231 · Cited by 368
Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics
Chen, K. G., Mallon, B. S., McKay, R. D. G., & Robey, P. G. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2013.12.005 · Cited by 358
Singh, A. M., Reynolds, D., Cliff, T., Ohtsuka, S., Mattheyses, A. L., Sun, Y., Menendez, L., Kulik, M., & Dalton, S. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2012.01.014 · Cited by 356
Katsuda, T., Kawamata, M., Hagiwara, K., Takahashi, R.-U., Yamamoto, Y., Camargo, F. D., & Ochiya, T. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2016.10.007 · Cited by 260
High-throughput approaches for the analysis of extrinsic regulators of stem cell fate
Ranga, A., & Lutolf, M. P. (2012) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2012.01.006 · Cited by 59
Metadata
- Authors
- Tsutsui, H., Valamehr, B., Hindoyan, A., Qiao, R., Ding, X., Guo, S., Witte, O. N., Liu, X., Ho, C.-M., & Wu, H.
- Venue
- Nature Communications
- Published
- 2011-01-25
- Volume
- 2
- Issue
- 1
- Pages
- 167-167
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2157713892
- Cited by
- 171
- References
- 5
- FWCI
- 7.64
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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