Hydrophobic surfaces for enhanced differentiation of embryonic stem cell-derived embryoid bodies
Open accessAbstract
With their unique ability to differentiate into all cell types, embryonic stem (ES) cells hold great therapeutic promise. To improve the efficiency of embryoid body (EB)-mediated ES cell differentiation, we studied murine EBs on the basis of their size and found that EBs with an intermediate size (diameter 100–300 μm) are the most proliferative, hold the greatest differentiation potential, and have the lowest rate of cell death. In an attempt to promote the formation of this subpopulation, we surveyed several biocompatible substrates with different surface chemical parameters and identified a strong correlation between hydrophobicity and EB development. Using self-assembled monolayers of various lengths of alkanethiolates on gold substrates, we directly tested this correlation and found that surfaces that exhibit increasing hydrophobicity enrich for the intermediate-size EBs. When this approach was applied to the human ES cell system, similar phenomena were observed. Our data demonstrate that hydrophobic surfaces serve as a platform to deliver uniform EB populations and may significantly improve the efficiency of ES cell differentiation.
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From Organoids (2022) · cites this paper as [70]
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- Authors
- Valamehr, B., Jonas, S. J., Polleux, J., Qiao, R., Guo, S., Gschweng, E. H., Stiles, B., Kam, K., Luo, T.-J. M., Witte, O. N., Liu, X., Dunn, B., & Wu, H.
- Affiliations
- *Department of Molecular and Medical Pharmacology, David Geffen School of Medicine,
- Department of Materials Science and Engineering, Henry Samueli School of Engineering and Applied Sciences,
- Department of Microbiology, Immunology, and Molecular Genetics,
- Howard Hughes Medical Institute (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2008-09-23
- Volume
- 105
- Issue
- 38
- Pages
- 14459-14464
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2567159
- OpenAlex
- W2027074821
- Cited by
- 155
- References
- 37
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 6 · 2023: 7 · 2022: 6 · 2021: 12 · 2020: 11 · 2019: 6 · 2018: 10 · 2017: 9 · 2016: 17 · 2015: 11 · 2014: 6 · 2013: 10 · 2012: 11
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (0114443)
- Howard Hughes Medical Institute
- National Institutes of Health
- National Eye Institute (PN2EY018228)
- National Institute of General Medical Sciences (T32GM008042)
- Division of Graduate Education
- California NanoSystems Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Keywords
- embryonic stem cells, embryoid bodies, hydrophobic surfaces, self-assembled monolayers, alkanethiolates, gold substrate, stem cell differentiation, surface chemistry, human embryonic stem cells, biocompatible substrates
- MeSH terms
- Animals, Cell Differentiation, Cell Survival, Dimethylpolysiloxanes, Humans, Culture Media, Serum-Free, Cell Culture Techniques, Cell Proliferation, Mice, Embryonic Stem Cells, Hydrophobic and Hydrophilic Interactions
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