Parallel evolution of the make–accumulate–consume strategy in Saccharomyces and Dekkera yeasts
Open accessAbstract
Saccharomyces yeasts degrade sugars to two-carbon components, in particular ethanol, even in the presence of excess oxygen. This characteristic is called the Crabtree effect and is the background for the 'make–accumulate–consume' life strategy, which in natural habitats helps Saccharomyces yeasts to out-compete other microorganisms. A global promoter rewiring in the Saccharomyces cerevisiae lineage, which occurred around 100 mya, was one of the main molecular events providing the background for evolution of this strategy. Here we show that the Dekkera bruxellensis lineage, which separated from the Saccharomyces yeasts more than 200 mya, also efficiently makes, accumulates and consumes ethanol and acetic acid. Analysis of promoter sequences indicates that both lineages independently underwent a massive loss of a specific cis-regulatory element from dozens of genes associated with respiration, and we show that also in D. bruxellensis this promoter rewiring contributes to the observed Crabtree effect. Saccharomycesyeasts can produce ethanol from sugars in the presence of oxygen. In this study, the authors demonstrate thatDekkera bruxellensis, a distantly related yeast, can also produce and consume ethanol due to the loss of a cis-regulatory element from the promoters of genes crucial for respiration.
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Yeast's primary function during the initial stage of liquid fermentation in fruit vinegar production is to convert sugars into ethanol and carbon dioxide, even under aerobic conditions.
“Yeast serves as a crucial microorganism during the initial stage of liquid fermentation in fruit vinegar production. Its primary function is to convert sugars into ethanol and carbon dioxide, even under aerobic conditions [45,46].”
From The Metabolic Pathways of Yeast and Acetic Acid Bacteria During Fruit Vinegar Fermentation and Their Influence on Flavor Development (2025) · cites this paper as [45]
The Results section reports that D. bruxellensis produced substantial ethanol under aerobic conditions, and the Abstract describes Saccharomyces yeasts converting sugars to ethanol despite excess oxygen. The paper does not address the initial stage of liquid fermentation in fruit vinegar production or establish that yeast’s primary function there is to produce ethanol and carbon dioxide. Its Results mention carbon dioxide in connection with a different yeast, B. naardenensis, for which ethanol was not detected. Page numbers were not available in our copy. (LLM, conf=0.87)
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- Authors
- Rozpędowska, E., Hellborg, L., Ishchuk, O. P., Orhan, F., Galafassi, S., Merico, A., Woolfit, M., Compagno, C., & Piškur, J.
- Affiliations
- Lund University (SE)
- University of Milan (IT)
- The University of Queensland (AU)
- Corresponding author
- Piškur, Jure
- Venue
- Nature Communications
- Published
- 2011-05-10
- Volume
- 2
- Issue
- 1
- Pages
- 302-302
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC3112538
- OpenAlex
- W2065692717
- Cited by
- 188
- References
- 2
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 17 · 2023: 14 · 2022: 8 · 2021: 9 · 2020: 19 · 2019: 11 · 2018: 13 · 2017: 17 · 2016: 12 · 2015: 17 · 2014: 10 · 2013: 12 · 2012: 13
- FWCI
- 5.07
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Funders
- Vetenskapsrådet
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Crabtree effect, Saccharomyces cerevisiae, Dekkera bruxellensis, ethanol production, acetic acid, parallel evolution, yeast evolution, oxygen tolerance
- MeSH terms
- Ethanol, Anaerobiosis, Base Sequence, DNA, Fungal, DNA, Mitochondrial, Biological Evolution, Fermentation, Glucose, Phylogeny, Promoter Regions, Genetic, RNA, Ribosomal, Saccharomyces cerevisiae, Sequence Analysis, DNA, Acetic Acid, Dekkera
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