Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms
Open accessAbstract
We analyzed 192 strains of the pathogenic yeast Candida glabrata from patients, mainly suffering from systemic infection, at Danish hospitals during 1985-1999. Our analysis showed that these strains were closely related but exhibited large karyotype polymorphism. Nine strains contained small chromosomes, which were smaller than 0.5 Mb. Regarding the year, patient and hospital, these C. glabrata strains had independent origin and the analyzed small chromosomes were structurally not related to each other (i.e. they contained different sets of genes). We suggest that at least two mechanisms could participate in their origin: (i) through a segmental duplication which covered the centromeric region, or (ii) by a translocation event moving a larger chromosome arm to another chromosome that leaves the centromere part with the shorter arm. The first type of small chromosomes carrying duplicated genes exhibited mitotic instability, while the second type, which contained the corresponding genes in only one copy in the genome, was mitotically stable. Apparently, in patients C. glabrata chromosomes are frequently reshuffled resulting in new genetic configurations, including appearance of small chromosomes, and some of these resulting "mutant" strains can have increased fitness in a certain patient "environment".
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The C. glabrata genome shows plasticity, and alterations in karyotype and chromosome size have been observed in clinical isolates and the reference strain CBS138.
“The C. glabrata genome shows plasticity, and alterations in karyotype and chromosome size have been observed in clinical isolates and the reference strain CBS138 [19,20,21].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [21]
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- Authors
- Ahmad, K. M., Ishchuk, O. P., Hellborg, L., Jørgensen, G., Skvarc, M., Stenderup, J., Jørck-Ramberg, D., Polakova, S., & Piškur, J.
- Affiliations
- Lund University (SE)
- Technical University of Denmark (DK)
- Regionshospitalet Herning (DK)
- Corresponding author
- Ahmad, Khadija Mohamed
- Venue
- Antonie van Leeuwenhoek
- Published
- 2013-05-14
- Volume
- 104
- Issue
- 1
- Pages
- 111-122
- ISSN
- 0003-6072 (ISSN-L)
- 1572-9699
- PMC
- PMC3672514
- OpenAlex
- W2070146936
- Cited by
- 29
- References
- 28
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2019: 4 · 2018: 4 · 2017: 1 · 2016: 4 · 2015: 3 · 2014: 4
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Antonie van Leeuwenhoek (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion, other-oa)
- Funders
- Vetenskapsrådet
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- Candida glabrata, small chromosomes, karyotype polymorphism, segmental duplication, centromeric region, chromosomal translocation, mitotic instability, pathogenic yeasts, systemic candidiasis, strain origin, genetic fitness
- MeSH terms
- Antifungal Agents, Base Sequence, Candidiasis, Cross Infection, Denmark, DNA, Fungal, DNA, Ribosomal, Genes, Fungal, Haploidy, Humans, Karyotyping, Molecular Sequence Data, Phylogeny, Selection, Genetic, Species Specificity, Translocation, Genetic, Fluconazole, Chromosomes, Fungal, Fungemia, Evolution, Molecular, Gene Duplication, Drug Resistance, Fungal, Candida glabrata, Genomic Instability
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