An internal deletion in MTH1 enables growth on glucose of pyruvate-decarboxylase negative, non-fermentative Saccharomyces cerevisiae

Oud, Bart; Flores, Carmen-Lisset; Gancedo, Carlos; Zhang, Xiuying; Trueheart, Joshua; Daran, Jean-Marc; Pronk, Jack T; van Maris, Antonius JA

doi:10.1186/1475-2859-11-131

Cited by 104Fungal and yeast genetics researchMicrobial Metabolic Engineering and BioproductionBiofuel production and bioconversion

An internal deletion in MTH1 enables growth on glucose of pyruvate-decarboxylase negative, non-fermentative Saccharomyces cerevisiae

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Oud, Bart, Flores, Carmen-Lisset, Gancedo, Carlos, Zhang, Xiuying, Trueheart, Joshua, Daran, Jean-Marc, Pronk, Jack T, van Maris, Antonius JA·2012·Microbial Cell Factories

DOI 10.1186/1475-2859-11-131PMC PMC3503853

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Abstract

Background: Pyruvate-decarboxylase negative (Pdc-) strains of Saccharomyces cerevisiae combine the robustness and high glycolytic capacity of this yeast with the absence of alcoholic fermentation. This makes Pdc- S. cerevisiae an interesting platform for efficient conversion of glucose towards pyruvate-derived products without formation of ethanol as a by-product. However, Pdc- strains cannot grow on high glucose concentrations and require C 2-compounds (ethanol or acetate) for growth under conditions with low glucose concentrations, which hitherto has limited application in industry. Results: Genetic analysis of a Pdc- strain previously evolved to overcome these deficiencies revealed a 225bp in-frame internal deletion in MTH1, encoding a transcriptional regulator involved in glucose sensing. This internal deletion contains a phosphorylation site required for degradation, thereby hypothetically resulting in increased stability of the protein. Reverse engineering of this alternative MTH1 allele into a non-evolved Pdc- strain enabled growth on 20 g l-1 glucose and 0.3% (v/v) ethanol at a maximum specific growth rate (0.24 h-1) similar to that of the evolved Pdc- strain (0.23 h-1). Furthermore, the reverse engineered Pdc- strain grew on glucose as sole carbon source, albeit at a lower specific growth rate (0.10 h-1) than the evolved strain (0.20 h-1). The observation that overexpression of the wild-type MTH1 allele also restored growth of Pdc- S. cerevisiae on glucose is consistent with the hypothesis that the internal deletion results in decreased degradation of Mth1. Reduced degradation of Mth1 has been shown to result in deregulation of hexose transport. In Pdc- strains, reduced glucose uptake may prevent intracellular accumulation of pyruvate and/or redox problems, while release of glucose repression due to the MTH1 internal deletion may contribute to alleviation of the C 2-compound auxotrophy. Conclusions: In this study we have discovered and characterised a mutation in MTH1 enabling Pdc- strains to grow on glucose as the sole carbon source. This successful example of reverse engineering not only increases the understanding of the glucose tolerance of evolved Pdc- S. cerevisiae, but also allows introduction of this portable genetic element into various industrial yeast strains, thereby simplifying metabolic engineering strategies.

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Sikorski, R. S., & Hieter, P. (1989) · Genetics · doi:10.1093/genetics/122.1.19 · Cited by 8,685 · see citing works
Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method
Gietz, R. D., & Woods, R. A. (2002) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(02)50957-5 · Cited by 2,890 · see citing works
Amino Acid Sequences Common to Rapidly Degraded Proteins: The PEST Hypothesis
Rogers, S. W., Wells, R., & RECHSTEINER, M. C. (1986) · Science · doi:10.1126/science.2876518 · Cited by 2,565 · see citing works
PEST sequences and regulation by proteolysis
RECHSTEINER, M. C., & Rogers, S. W. (1996) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(96)10031-1 · Cited by 1,645 · see citing works
Effect of benzoic acid on metabolic fluxes in yeasts: A continuous‐culture study on the regulation of respiration and alcoholic fermentation
Verduyn, C., Postma, E., Scheffers, W. A., & van Dijken, J. P. (1992) · Yeast · doi:10.1002/yea.320080703 · Cited by 1,510 · see citing works
Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
Rothstein, R. (1991) · Methods in Enzymology · doi:10.1016/0076-6879(91)94022-5 · Cited by 1,283 · see citing works
Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin
Westfall, P. J., Pitera, D. J., Lenihan, J. R., Eng, D., Woolard, F. X., Regentin, R., Horning, T., Tsuruta, H., Melis, D. J., Owens, A. E., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1110740109 · Cited by 726 · see citing works
An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains
van Dijken, J. P., Bauer, J., Brambilla, L., Duboc, P., Francois, J. M., Gancedo, C., Giuseppin, M. L. F., Heijnen, J. J., Hoare, M., Lange, H. C., et al. (2000) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(00)00162-9 · Cited by 598 · see citing works
Self-citation
Progress in Metabolic Engineering of Saccharomyces cerevisiae
Nevoigt, E. (2008) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00025-07 · Cited by 597 · see citing works
Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status
van Maris, A. J. A., Abbott, D. A., Bellissimi, E., van den Brink, J., Kuyper, M., Luttik, M. A. H., Wisselink, H. W., Scheffers, W. A., van Dijken, J. P., & Pronk, J. T. (2006) · Antonie van Leeuwenhoek · doi:10.1007/s10482-006-9085-7 · Cited by 546 · see citing works
Self-citation
How did Saccharomyces evolve to become a good brewer?
Piškur, J., Rozpędowska, E., Poláková, S., Merico, A., & Compagno, C. (2006) · Trends in Genetics · doi:10.1016/j.tig.2006.02.002 · Cited by 514 · see citing works
Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries
Hong, K.-K., & Nielsen, J. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-0945-1 · Cited by 451 · see citing works
Three Different Regulatory Mechanisms Enable Yeast Hexose Transporter ( HXT ) Genes To Be Induced by Different Levels of Glucose
Özcan, S., & Johnston, M. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.3.1564 · Cited by 382 · see citing works
Glucose sensing and signaling by two glucose receptors in the yeast Saccharomyces cerevisiae
Özcan, S., Dover, J., & Johnston, M. (1998) · The EMBO Journal · doi:10.1093/emboj/17.9.2566 · Cited by 359 · see citing works
Resurrecting ancestral alcohol dehydrogenases from yeast
Thomson, J. M., Gaucher, E. A., Burgan, M. F., De Kee, D. W., Li, T., Aris, J. P., & Benner, S. A. (2005) · Nature Genetics · doi:10.1038/ng1553 · Cited by 341 · see citing works
Trends and challenges in the microbial production of lignocellulosic bioalcohol fuels
Weber, C., Farwick, A., Benisch, F., Brat, D., Dietz, H., Subtil, T., & Boles, E. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2707-z · Cited by 335 · see citing works
25 Yeast Genetic Strain and Plasmid Collections
Entian, K., & Kötter, P. (2007) · Methods in microbiology · doi:10.1016/s0580-9517(06)36025-4 · Cited by 310 · see citing works
The early steps of glucose signalling in yeast.
Gancedo, J. (2008) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2008.00117.x · Cited by 249 · see citing works
Development of Efficient Xylose Fermentation in Saccharomyces cerevisiae: Xylose Isomerase as a Key Component
van Maris, A. J. A., Winkler, A. A., Kuyper, M., de Laat, W. T. A. M., van Dijken, J. P., & Pronk, J. T. (2007) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2007_057 · Cited by 245 · see citing works
Self-citation
Glucose sensing and signaling in Saccharomyces cerevisiae through the Rgt2 glucose sensor and casein kinase I
Moriya, H., & Johnston, M. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0305901101 · Cited by 242 · see citing works
Directed Evolution of Pyruvate Decarboxylase-Negative Saccharomyces cerevisiae , Yielding a C 2 -Independent, Glucose-Tolerant, and Pyruvate-Hyperproducing Yeast
van Maris, A. J. A., Geertman, J.-M. A., Vermeulen, A., Groothuizen, M. K., Winkler, A. A., Piper, M. D. W., van Dijken, J. P., & Pronk, J. T. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.1.159-166.2004 · Cited by 227 · cited 1× · see citing works
“The goal of the present study was to identify the mutation(s) responsible for the ability of the evolved Pdc- strain isolated by Van Maris et al. (2004) to grow on high concentrations of glucose as sole carbon source.”
BackgroundSelf-citation
Microbial export of lactic and 3-hydroxypropanoic acid: implications for industrial fermentation processes
van Maris, A. J. A., Konings, W. N., van Dijken, J. P., & Pronk, J. T. (2004) · Metabolic Engineering · doi:10.1016/j.ymben.2004.05.001 · Cited by 190 · see citing works
Self-citation
Characterization of PDC6, a third structural gene for pyruvate decarboxylase in Saccharomyces cerevisiae
Hohmann, S. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.24.7963-7969.1991 · Cited by 189 · see citing works
Parallel evolution of the make–accumulate–consume strategy in Saccharomyces and Dekkera yeasts
Rozpędowska, E., Hellborg, L., Ishchuk, O. P., Orhan, F., Galafassi, S., Merico, A., Woolfit, M., Compagno, C., & Piškur, J. (2011) · Nature Communications · doi:10.1038/ncomms1305 · Cited by 188 · see citing works
Regulatory Network Connecting Two Glucose Signal Transduction Pathways in Saccharomyces cerevisiae
Kaniak, A., Xue, Z., Macool, D. J., Kim, J., & Johnston, M. (2004) · Eukaryotic Cell · doi:10.1128/ec.3.1.221-231.2004 · Cited by 166 · see citing works
Metabolic engineering ofSaccharomyces cerevisiae for production of carboxylic acids: current status and challenges
Abbott, D. A., Zelle, R. M., Pronk, J. T., & van Maris, A. J. A. (2009) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2009.00537.x · Cited by 164 · see citing works
Self-citation
Grr1-dependent Inactivation of Mth1 Mediates Glucose-induced Dissociation of Rgt1 from HXT Gene Promoters
Flick, K. M., Spielewoy, N., Kalashnikova, T. I., Guaderrama, M., Zhu, Q., Chang, H.-C., & Wittenberg, C. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-03-0135 · Cited by 159 · see citing works
Rgt1p of Saccharomyces cerevisiae , a Key Regulator of Glucose-Induced Genes, Is both an Activator and a Repressor of Transcription
Özcan, S., Leong, T., & Johnston, M. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.11.6419 · Cited by 157 · see citing works
Stepwise increase of resveratrol biosynthesis in yeast Saccharomyces cerevisiae by metabolic engineering
Wang, Y., Halls, C. E., Zhang, J., Matsuno, M., Zhang, Y., & Yu, O. S. (2011) · Metabolic Engineering · doi:10.1016/j.ymben.2011.04.005 · Cited by 135 · see citing works
Specificity and Regulation of DNA Binding by the Yeast Glucose Transporter Gene Repressor Rgt1
Kim, J., Polish, J. A., & Johnston, M. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.15.5208-5216.2003 · Cited by 129 · see citing works
Homofermentative Lactate Production Cannot Sustain Anaerobic Growth of Engineered Saccharomyces cerevisiae : Possible Consequence of Energy-Dependent Lactate Export
van Maris, A. J. A., Winkler, A. A., Porro, D., van Dijken, J. P., & Pronk, J. T. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.5.2898-2905.2004 · Cited by 123 · see citing works
Self-citation
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Jackson, B. E., Hart-Wells, E. A., & Matsuda, S. P. T. (2003) · Organic Letters · doi:10.1021/ol034231x · Cited by 118 · see citing works
Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae
Flikweert, M. T., de Swaaf, M. E., van Dijken, J. P., & Pronk, J. T. (1999) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1999.tb13551.x · Cited by 108 · see citing works
Self-citation
Repression of transcription by Rgt1 in the absence of glucose requires Std1 and Mth1
Lakshmanan, J., Mosley, A. L., & �zcan, S. (2003) · Current Genetics · doi:10.1007/s00294-003-0423-2 · Cited by 106 · see citing works
Mth1 receives the signal given by the glucose sensors Snf3 and Rgt2 in Saccharomyces cerevisiae ‡
Lafuente, M. J., Gancedo, C., Jauniaux, J. C., & Gancedo, J. M. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.01688.x · Cited by 102 · see citing works
Self-citation
Redirection of the Respiro-Fermentative Flux Distribution in Saccharomyces cerevisiae by Overexpression of the Transcription Factor Hap4p
Blom, J., de Mattos, M. J. T., & Grivell, L. A. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.5.1970-1973.2000 · Cited by 94 · see citing works
How the Rgt1 Transcription Factor of Saccharomyces cerevisiae Is Regulated by Glucose
Polish, J. A., Kim, J., & Johnston, M. (2004) · Genetics · doi:10.1534/genetics.104.034512 · Cited by 94 · see citing works
Systems biology of yeast: enabling technology for development of cell factories for production of advanced biofuels
de Jong, B. W., Siewers, V., & Nielsen, J. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.11.021 · Cited by 93 · see citing works
Genome-wide analytical approaches for reverse metabolic engineering of industrially relevant phenotypes in yeast
Oud, B., Maris, A. J. A., Daran, J.-M., & Pronk, J. T. (2012) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2011.00776.x · Cited by 82 · see citing works
Self-citation
Recent Advances in Artemisinin Production Through Heterologous Expression
Arsenault, P. R., Wobbe, K. K., & Weathers, P. J. (2008) · Current Medicinal Chemistry · doi:10.2174/092986708786242813 · Cited by 79 · see citing works
Investigation of the impact of 1 and 2 on the physiology of
KLEIN, C., RASMUSSEN, J., Rønnow, B., Lisbeth Olsson, A., & Nielsen, J. (1999) · Journal of Biotechnology · doi:10.1016/s0168-1656(98)00205-3 · Cited by 73 · see citing works
Chemostat-Based Micro-Array Analysis in Baker's Yeast
Daran-Lapujade, P. A. S., Daran, J.-M. G., van Maris, A. J. A., de Winde, J. H., & Pronk, J. T. (2008) · Advances in microbial physiology/Advances in Microbial Physiology · doi:10.1016/s0065-2911(08)00004-0 · Cited by 71 · see citing works
Self-citation
Active Snf1 protein kinase inhibits expression of the Saccharomyces cerevisiae HXT1 glucose transporter gene
Tomás‐Cobos, L., & Sanz, P. (2002) · Biochemical Journal · doi:10.1042/bj20020984 · Cited by 71 · see citing works
Key Process Conditions for Production of C 4 Dicarboxylic Acids in Bioreactor Batch Cultures of an Engineered Saccharomyces cerevisiae Strain
Zelle, R. M., de Hulster, E. A. F., Kloezen, W., Pronk, J. T., & van Maris, A. J. A. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.02396-09 · Cited by 69 · see citing works
Self-citation
Glucose uptake and catabolite repression in dominant HTR1 mutants of Saccharomyces cerevisiae
Özcan, S., Freidel, K., Leuker, A., & Ciriacy, M. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.17.5520-5528.1993 · Cited by 66 · see citing works
Laboratory evolution of new lactate transporter genes in a jen1Δ mutant of Saccharomyces cerevisiae and their identification as ADY2 alleles by whole-genome resequencing and transcriptome analysis
Kok, S., Nijkamp, J. F., Oud, B., Roque, F. C., Ridder, D., Daran, J.-M., Pronk, J. T., & Maris, A. J. A. (2012) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2011.00787.x · Cited by 65 · see citing works
Self-citation
Anaplerotic Role for Cytosolic Malic Enzyme in Engineered Saccharomyces cerevisiae Strains
Zelle, R. M., Harrison, J. C., Pronk, J. T., & van Maris, A. J. A. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.02132-10 · Cited by 58 · see citing works
Self-citation
The mutation DGT1-1 decreases glucose transport and alleviates carbon catabolite repression in Saccharomyces cerevisiae
Gamo, F., Lafuente, M. J., & Gancedo, C. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.24.7423-7429.1994 · Cited by 56 · see citing works
Self-citation
Construction of a set of Saccharomyces cerevisiae vectors designed for recombinational cloning
Mullem, V. V., Wéry, M., Bolle, X. D., & Vandenhaute, J. (2003) · Yeast · doi:10.1002/yea.999 · Cited by 55 · see citing works
Malolactic Fermentation by Engineered Saccharomyces cerevisiae as Compared with Engineered Schizosaccharomyces pombe
Ansanay, V., Dequin, S., Camarasa, C., Schaeffer, V. H., Grivet, J. P., Blondin, B., Salmon, J.-M., & Barre, P. (1996) · Yeast · doi:10.1002/(sici)1097-0061(19960315)12:3 · Cited by 53 · see citing works
Self-citation
The HTR1 Gene Is a Dominant Negative Mutant Allele of MTH1 and Blocks Snf3- and Rgt2-Dependent Glucose Signaling in Yeast
Schulte, F., Wieczorke, R., Hollenberg, C. P., & Boles, E. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.2.540-542.2000 · Cited by 47 · see citing works
Functional dissection of the glucose signaling pathways that regulate the yeast glucose transporter gene (HXT) repressor Rgt1
Jouandot, D. J., Roy, A., & Kim, J. (2011) · Journal of Cellular Biochemistry · doi:10.1002/jcb.23253 · Cited by 44 · see citing works
A quantitative model of glucose signaling in yeast reveals an incoherent feed forward loop leading to a specific, transient pulse of transcription
Kuttykrishnan, S., Sabina, J., Langton, L., Johnston, M., & Brent, M. R. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0912483107 · Cited by 40 · see citing works
Asymmetric Signal Transduction through Paralogs That Comprise a Genetic Switch for Sugar Sensing in Saccharomyces cerevisiae
Sabina, J., & Johnston, M. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.032102 · Cited by 31 · see citing works
A mutation affecting carbon catabolite repression suppresses growth defects in pyruvate carboxylase mutants from Saccharomyces cerevisiae
Blázquez, M. A., Gamo, F., & Gancedo, C. (1995) · FEBS Letters · doi:10.1016/0014-5793(95)01337-7 · Cited by 30 · see citing works
Self-citation
DNA-binding properties of the yeast Rgt1 repressor
Kim, J. (2008) · Biochimie · doi:10.1016/j.biochi.2008.09.002 · Cited by 27 · see citing works
Effects of a hexokinase II deletion on the dynamics of glycolysis in continuous cultures ofSaccharomyces cerevisiae
Diderich, J. A., Raamsdonk, L. M., Kuiper, A., Kruckeberg, A. L., BERDEN, J. A., de Mattos, M. J. T., & DAM, K. V. (2002) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2002.tb00081.x · Cited by 17 · see citing works
1996: A vintage year for yeast and Yeast
Goffeau, A. (1996) · Yeast · doi:10.1002/(sici)1097-0061(199612)12:16 · Cited by 2 · see citing works
Untitled work
(1996) · doi:10.1002/(sici)1097-0290(19961005)52:1

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Wess, J., Brinek, M., & Boles, E. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1486-8 · Cited by 103 · see citing works
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Yan, Q., & Pfleger, B. F. (2020) · Metabolic Engineering · doi:10.1016/j.ymben.2019.04.009 · Cited by 105 · see citing works
Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities
Kim, J. W., Kim, J., Seo, S.-O., Kim, K. H., Jin, Y., & Seo, J. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0677-9 · Cited by 94 · see citing works
Metabolic engineering of Saccharomyces cerevisiae for production of butanol isomers
Generoso, W. C., Schadeweg, V., Oreb, M., & Boles, E. (2014) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2014.09.004 · Cited by 94 · see citing works
Evolution reveals a glutathione-dependent mechanism of 3-hydroxypropionic acid tolerance
Kildegaard, K. R., Hallström, B. M., Blicher, T. H., Sonnenschein, N., Jensen, N. B., Sherstyk, S., Harrison, S. J., Maury, J., Herrgård, M. J., Juncker, A. S., et al. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.09.004 · Cited by 83 · see citing works
Engineering Saccharomyces cerevisiae cells for production of fatty acid-derived biofuels and chemicals
Hu, Y., Zhu, Z., Nielsen, J., & Siewers, V. (2019) · Open Biology · doi:10.1098/rsob.190049 · Cited by 81 · see citing works
Directed Enzyme Evolution: Advances and Applications
Alcalde, M. (2017) · Springer eBooks · doi:10.1007/978-3-319-50413-1 · Cited by 80 · see citing works
Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein degradation
González-Ramos, D., van den Broek, M. A., van Maris, A. J. A., Pronk, J. T., & Daran, J.-M. G. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-48 · Cited by 78 · see citing works
Self-citation
Yeast factories for the production of aromatic compounds: from building blocks to plant secondary metabolites
Suástegui, M., & Shao, Z. (2016) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-016-1824-9 · Cited by 78 · see citing works
Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production
Zhang, Y., Cortez, J. D., Hammer, S. K., Carrasco‐López, C., Echauri, S. A. G., Wiggins, J. B., Wang, W., & Avalos‬, J. L. (2022) · Nature Communications · doi:10.1038/s41467-021-27852-x · Cited by 71 · see citing works
Expression of Lactococcus lactis NADH oxidase increases 2,3-butanediol production in Pdc-deficient Saccharomyces cerevisiae
Kim, J.-W., Seo, S., Zhang, G.-C., Jin, Y., & Seo, J. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.02.077 · Cited by 66 · see citing works
Rewiring yeast metabolism to synthesize products beyond ethanol
Gambacorta, F. V., Dietrich, J. J., Yan, Q., & Pfleger, B. F. (2020) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2020.08.005 · Cited by 64 · see citing works
Pentose metabolism and conversion to biofuels and high-value chemicals in yeasts
Ruchała, J., & Sibirny, A. А. (2020) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuaa069 · Cited by 64 · see citing works
Metabolic engineering of Saccharomyces cerevisiae for 2,3-butanediol production
Kim, S.-J., Kim, J.-W., Lee, Y., Park, Y., & Seo, J. (2017) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-017-8172-1 · Cited by 63 · see citing works
Pathway engineering for the production of heterologous aromatic chemicals and their derivatives in Saccharomyces cerevisiae: bioconversion from glucose
Gottardi, M., Reifenrath, M., Boles, E., & Tripp, J. (2017) · FEMS Yeast Research · doi:10.1093/femsyr/fox035 · Cited by 62 · see citing works
Ach1 is involved in shuttling mitochondrial acetyl units for cytosolic C2 provision in Saccharomyces cerevisiae lacking pyruvate decarboxylase
Chen, Y., Zhang, Y., Siewers, V., & Nielsen, J. (2015) · FEMS Yeast Research · doi:10.1093/femsyr/fov015 · Cited by 58 · see citing works
Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose
Zhang, B., Zhu, Y., Zhang, J., Wang, D., Sun, L., & Hong, J. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.11.110 · Cited by 47 · see citing works
Engineering of Saccharomyces cerevisiae for enhanced metabolic robustness and L-lactic acid production from lignocellulosic biomass
Choi, B.-H., Rangel, A. E. T., Kerkhoven, E. J., & Nygård, Y. (2024) · Metabolic Engineering · doi:10.1016/j.ymben.2024.05.003 · Cited by 46 · see citing works
Pyruvate metabolism redirection for biological production of commodity chemicals in aerobic fungus Aspergillus oryzae
Zhang, S., Wakai, S., Sasakura, N., Tsutsumi, H., Hata, Y., Ogino, C., & Kondo, A. (2020) · Metabolic Engineering · doi:10.1016/j.ymben.2020.06.010 · Cited by 45 · see citing works
Adaptive mutations in sugar metabolism restore growth on glucose in a pyruvate decarboxylase negative yeast strain
Zhang, Y., Liu, G., Engqvist, M. K. M., Krivoruchko, A., Hallström, B. M., Chen, Y., Siewers, V., & Nielsen, J. (2015) · Microbial Cell Factories · doi:10.1186/s12934-015-0305-6 · Cited by 43 · see citing works
A pyruvate carbon flux tugging strategy for increasing 2,3-butanediol production and reducing ethanol subgeneration in the yeast Saccharomyces cerevisiae
Ishii, J., Morita, K., Ida, K., Kato, H., Kinoshita, S., Hataya, S., Shimizu, H., Kondo, A., & Matsuda, F. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1176-y · Cited by 42 · see citing works
Excessive by-product formation: A key contributor to low isobutanol yields of engineered Saccharomyces cerevisiae strains
Milne, N., Wahl, A., van Maris, A. J. A., Pronk, J. T., & Daran, J.-M. G. (2016) · Metabolic Engineering Communications · doi:10.1016/j.meteno.2016.01.002 · Cited by 37 · see citing works
Self-citation
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Zhang, Y., Su, M., Wang, Z., Nielsen, J., & Liu, Z. (2022) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2022.06.004 · Cited by 35 · see citing works
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Cui, D.-Y., Wei, Y., Lin, L., Chen, S.-J., Feng, P.-P., Xiao, D., Lin, X., & Zhang, C. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.596306 · Cited by 35 · see citing works
High-copy genome integration of 2,3-butanediol biosynthesis pathway in Saccharomyces cerevisiae via in vivo DNA assembly and replicative CRISPR-Cas9 mediated delta integration
Huang, S., & Geng, A. (2020) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2020.01.014 · Cited by 32 · see citing works
Metabolic engineering of non-pathogenic microorganisms for 2,3-butanediol production
Lee, J., Lee, Y., Jin, Y., & Rao, C. V. (2021) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-021-11436-2 · Cited by 30 · see citing works
The advances in creating Crabtree-negative Saccharomyces cerevisiae and the application for chemicals biosynthesis
Guo, Y., Xiong, Z., Zhai, H., Wang, Y., Qi, Q., & Hou, J. (2025) · FEMS Yeast Research · doi:10.1093/femsyr/foaf014 · Cited by 25 · see citing works
GSF2 deletion increases lactic acid production by alleviating glucose repression in Saccharomyces cerevisiae
Baek, S., Kwon, E. Y., Kim, S., & Hahn, J. (2016) · Scientific Reports · doi:10.1038/srep34812 · Cited by 23 · see citing works
Secretion of 2,3-dihydroxyisovalerate as a limiting factor for isobutanol production in Saccharomyces cerevisiae
Generoso, W. C., Brinek, M., Dietz, H., Oreb, M., & Boles, E. (2017) · FEMS Yeast Research · doi:10.1093/femsyr/fox029 · Cited by 22 · see citing works
Bioengineering for the industrial production of 2,3-butanediol by the yeast, Saccharomyces cerevisiae
Mitsui, R., Yamada, R., Matsumoto, T., & Ogino, H. (2022) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-021-03224-x · Cited by 21 · see citing works
Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae
Milne, N., van Maris, A. J. A., Pronk, J. T., & Daran, J.-M. G. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0374-0 · Cited by 21 · see citing works
Self-citation
In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01594-3 · Cited by 21 · see citing works
Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth
Zhang, Y., Dai, Z., Krivoruchko, A., Chen, Y., Siewers, V., & Nielsen, J. (2015) · FEMS Yeast Research · doi:10.1093/femsyr/fov024 · Cited by 20 · see citing works
Enhanced production of 2,3‐butanediol in pyruvate decarboxylase‐deficient Saccharomyces cerevisiae through optimizing ratio of glucose/galactose
Choi, E., Kim, J., Kim, S., Seo, S., Lane, S., Park, Y., Jin, Y., & Seo, J. (2016) · Biotechnology Journal · doi:10.1002/biot.201600042 · Cited by 19 · see citing works
Combination of Three Methods to Reduce Glucose Metabolic Rate For Improving N-Acetylglucosamine Production in Saccharomyces cerevisiae
Lee, S., Lee, B.-Y., & Oh, M. (2018) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.8b04291 · Cited by 19 · see citing works
Improvement of whole-cell transamination with Saccharomyces cerevisiae using metabolic engineering and cell pre-adaptation
Weber, N. F., Gorwa‐Grauslund, M. F., & Carlquist, M. (2017) · Microbial Cell Factories · doi:10.1186/s12934-016-0615-3 · Cited by 18 · see citing works
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Chen, H., Chai, X., Wang, Y., Liu, J. L., Zhou, G., Wei, P., Song, Y., & Ma, L. (2022) · Microbial Cell Factories · doi:10.1186/s12934-022-01900-7 · Cited by 16 · see citing works
Serial propagation in water-in-oil emulsions selects for Saccharomyces cerevisiae strains with a reduced cell size or an increased biomass yield on glucose
van Tatenhove‐Pel, R. J., Zwering, E., Boreel, D. F., Falk, M., van Heerden, J. H., Kes, M. B. M. J., Kranenburg, C. I., Botman, D., Teusink, B., & Bachmann, H. (2021) · Metabolic Engineering · doi:10.1016/j.ymben.2020.12.005 · Cited by 15 · see citing works
A Review on the Production of C4 Platform Chemicals from Biochemical Conversion of Sugar Crop Processing Products and By-Products
Bruni, G. O., & Terrell, E. (2022) · Fermentation · doi:10.3390/fermentation8050216 · Cited by 12 · see citing works
First aspects on acetate metabolism in the yeast Dekkera bruxellensis: a few keys for improving ethanol fermentation
Teles, G. H., da Silva, J. M., Mendonça, A. A., de Morais, M. A., & Pita, W. D. B. (2018) · Yeast · doi:10.1002/yea.3348 · Cited by 12 · see citing works
Co-Production of Isobutanol and Ethanol from Prairie Grain Starch Using Engineered Saccharomyces cerevisiae
Liu, X., Unaegbunam, E., & Stuart, D. T. (2021) · Fermentation · doi:10.3390/fermentation7030150 · Cited by 12 · see citing works
A highly efficient transcriptome-based biosynthesis of non-ethanol chemicals in Crabtree negative Saccharomyces cerevisiae
Yao, Z., Guo, Y., Wang, H., Chen, Y., Wang, Q., Nielsen, J., & Dai, Z. (2023) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-023-02276-5 · Cited by 11 · see citing works
Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
Tönjes, S., Uitterhaegen, E., Palmans, I., Ibach, B., Winter, K. D., Dijck, P. V., Soetaert, W., & Vandecruys, P. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms251910245 · Cited by 10 · see citing works
Switching the yeast metabolism via manipulation of sugar phosphorylation
Fan, C., Fan, J., Chen, H., Lin, S., Zhang, D., Song, J., Wang, J., Wang, Y., Xiao, H., & Yuan, J. (2025) · Metabolic Engineering · doi:10.1016/j.ymben.2025.02.008 · Cited by 10 · see citing works
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Sjöberg, G., Reķēna, A., Fornstad, M., Lahtvee, P., & van Maris, A. J. A. (2024) · Metabolic Engineering · doi:10.1016/j.ymben.2024.01.007 · Cited by 9 · see citing works
Elimination of aromatic fusel alcohols as by-products of Saccharomyces cerevisiae strains engineered for phenylpropanoid production by 2-oxo-acid decarboxylase replacement
Hassing, E.-J., Buijs, J., Blankerts, N., Luttik, M. A. H., de Hulster, E. A. F., Pronk, J. T., & Daran, J.-M. G. (2021) · Metabolic Engineering Communications · doi:10.1016/j.mec.2021.e00183 · Cited by 9 · see citing works
Self-citation
Metabolic Engineering Strategies to Convert Carbohydrates to Aviation Range Hydrocarbons
Bergman, A., & Siewers, V. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-804568-8.00007-x · Cited by 9 · see citing works
Strain Development by Whole-Cell Directed Evolution
Si, T., Lian, J., & Zhao, H. (2017) · Springer eBooks · doi:10.1007/978-3-319-50413-1_7 · Cited by 7 · see citing works
The novel properties of Kluyveromyces marxianus glucose sensor/receptor repressor pathway and the construction of glucose repression-released strains
Wang, L., Wang, A., Wang, D., & Hong, J. (2023) · Microbial Cell Factories · doi:10.1186/s12934-023-02138-7 · Cited by 7 · see citing works
The Transcriptome and Flux Profiling of Crabtree‐Negative Hydroxy Acid‐Producing Strains of Saccharomyces cerevisiae Reveals Changes in the Central Carbon Metabolism
Jessop-Fabre, M. M., Dahlin, J., Biron, M. B., Šťovíček, V., Ebert, B. E., Blank, L. M., Budin, I., Keasling, J. D., & Borodina, I. (2019) · Biotechnology Journal · doi:10.1002/biot.201900013 · Cited by 4 · see citing works
Expression of Saccharomyces cerevisiae cDNAs to enhance the growth of non-ethanol-producing S. cerevisiae strains lacking pyruvate decarboxylases
Narazaki, Y., Nomura, Y., Morita, K., Shimizu, H., & Matsuda, F. (2018) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2018.03.008 · Cited by 3 · see citing works
In situ engineering of synthetic yeast consortia for cross-species metabolic conversion of crude glycerol and byproducts into circular renewable bioenergy
Shi, X., Chang, J., Lee, M.-E., Oh, J. W., Hwang, D.-H., Cho, B.-H., & Han, S. O. (2026) · Trends in biotechnology · doi:10.1016/j.tibtech.2025.12.002 · Cited by 2 · see citing works
Systems Biology: Developments and Applications
Kumar, R., Lahtvee, P., & Nielsen, J. (2014) · Springer eBooks · doi:10.1007/978-3-642-55013-3_4 · Cited by 2 · see citing works
Genetically encoded biosensors for branched-chain amino acid metabolism to monitor mitochondrial and cytosolic production of isobutanol and isopentanol in yeastPreprint
Zhang, Y., Hammer, S. K., Carrasco‐López, C., Echauri, S. A. G., & Avalos‬, J. L. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.03.08.982801 · Cited by 2 · see citing works
Bio-Based Commodity Chemicals Via Genetically Engineered Microbes
Mishra, S., Perkovich, P. M., & Pfleger, B. F. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-15742-4.00088-0 · Cited by 2 · see citing works
Creating an oil yeast from brewing yeast
Hou, J. (2018) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2018.10.007 · Cited by 1 · see citing works
Improving microbial chemical production strains through transcriptional regulatory network rewiring
Rodrigues, R. (2015) · doi:10.25560/30834 · Cited by 1 · see citing works
Corrigendum to “Rewiring yeast metabolism to synthesize products beyond ethanol” [Curr Opin Chem Biol 59 (December 2020) 182–192]
Gambacorta, F. V., Dietrich, J. J., Yan, Q., & Pfleger, B. F. (2020) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2020.10.006 · Cited by 1 · see citing works
Toward Fermentative Production of Succinic Acid, Adipic Acid, and Caprolactam: An Industrial Perspective
Wu, L. (2016) · Green chemistry and sustainable technology · doi:10.1007/978-3-662-53704-6_3
Yeast engineering for pharmaceutical and nutraceutical purposes
Kwak, S. (2017) · Illinois Digital Environment for Access to Learning and Scholarship (University of Illinois at Urbana-Champaign)
Engineering Natural Product Biosynthetic Pathways to Produce Commodity and Specialty Chemicals
Liu, Y., Gan, F., Cruz‐Morales, P., & Keasling, J. D. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-409547-2.14825-5
Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in
Nielsen, Y. (2014)
Cellular heterogeneity and MTH1 play key roles in galactose mediated signaling of the GAL switch to utilize the disaccharide melibiosePreprint
Kar, R. K., & Bhat, P. J. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.06.16.448739
Analisis Pengaruh Persepsi, Tingkat Pendidikan dan Etos Kerja terhadap Efektivitas Aplikasi Sistem Manajemen Keuangan Daerah (Studi Kasus Pengelola Laporan Keuangan Daerah di Lingkungan Sekretariat Daerah Kabupaten Rembang)
Wijayanti, R., Nugraheni, F., & Ansori, M. (2010)
Faculty Opinions recommendation of Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative.
Bähler, J., & Małecki, M. (2020) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.733757636.793569522
HP1-enhanced chromatin compaction stabilizes a synthetic metabolic circuit in yeastPreprint
González, L., Echauri, S. A. G., Jerónimo, C., Poitras, C., Gencel, M., Serohijos, A. W. R., Bloom, K., Robert, F., Avalos‬, J. L., & Michnick, S. W. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.03.04.641524
Additional file 4 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746515
Additional file 17 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746500
Additional file 13 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746488
Additional file 9 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746530
Additional file 6 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746521
Additional file 1 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746506
Additional file 5 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746518
Additional file 18 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746503
Additional file 2 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746509
Additional file 3 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746512
Additional file 8 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746527
Additional file 16 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746497
Additional file 7 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746524
Additional file 12 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746485
Additional file 15 of In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain
Nicolaï, T., Deparis, Q., Foulquié-Moreno, M. R., & Thevelein, J. M. (2021) · Figshare · doi:10.6084/m9.figshare.14746494

Adaptive mutations in sugar metabolism restore growth on glucose in a pyruvate decarboxylase negative yeast strain
Zhang, Y., Liu, G., Engqvist, M. K. M., Krivoruchko, A., Hallström, B. M., Chen, Y., Siewers, V., & Nielsen, J. (2015) · Microbial Cell Factories · doi:10.1186/s12934-015-0305-6 · Cited by 43 · see citing works
Engineering cytosolic acetyl-CoA metabolism in Saccharomyces cerevisiae
Zhang, Y. (2015) · Chalmers Research (Chalmers University of Technology)
Directed evolution of pyruvate decarboxylase-negative Saccharomyces cerevisiae, yielding a C2-independent, glucose-tolerant, and pyruvate-hyperproducing yeast
van Maris, A. J. A., Geertman, J.-M. A., Vermeulen, A., Groothuizen, M. K., Winkler, A., Piper, M. D. W., van Dijken, J. P., & Pronk, J. T. (2004) · RePub (Erasmus University Rotterdam)
Self-citation
Malolactic Fermentation by Engineered Saccharomyces cerevisiae as Compared with Engineered Schizosaccharomyces pombe
Ansanay, V., Dequin, S., Camarasa, C., Schaeffer, V. H., Grivet, J. P., Blondin, B., Salmon, J.-M., & Barre, P. (1996) · Yeast · doi:10.1002/(sici)1097-0061(19960315)12:3 · Cited by 53 · see citing works
Self-citation
RESEARCH Open Access
Pronk, J. T., & van Maris, A. J. A. (2016)
Self-citation

Authors: Oud, B., Flores, C.-L., Gancedo, C., Zhang, X., Trueheart, J., Daran, J.-M., Pronk, J. T., & van Maris, A. J.
Affiliations: Delft University of Technology (NL)
Instituto de Investigaciones Biomédicas Sols-Morreale (ES)
ORCID: Flores, Carmen-Lisset
Zhang, Xiuying
Daran, Jean-Marc
Pronk, Jack T
Venue: Microbial Cell Factories
Publisher: Springer Science and Business Media LLC
Published: 2012-09-15
Volume: 11
Issue: 1
Pages: 131-131
ISSN: 1475-2859 (ISSN-L)
DOI: 10.1186/1475-2859-11-131
PMC: PMC3503853
OpenAlex: W2127470474
Cited by: 104
References: 61
Citations by year: 2026: 2 · 2025: 3 · 2024: 5 · 2023: 2 · 2022: 5 · 2021: 24 · 2020: 9 · 2019: 4 · 2018: 7 · 2017: 8 · 2016: 11 · 2015: 7 · 2014: 10 · 2013: 6
FWCI: 2.96
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
Locations: Microbial Cell Factories (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Research Repository (Delft University of Technology) (submittedVersion, other-oa)
Data Archiving and Networked Services (DANS) (submittedVersion, cc-by)
Funders: Ministerio de Ciencia e Innovación
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Keywords: MTH1, Saccharomyces cerevisiae, glucose tolerance, glucose detection, transcription factors, protein stability, phosphorylation sites, reverse engineering, glucose repression, ethanol tolerance, glucose uptake, hexose transport, metabolic engineering
MeSH terms: Alleles, Amino Acid Sequence, Fermentation, Glucose, Molecular Sequence Data, Mutation, Pyruvate Decarboxylase, Saccharomyces cerevisiae, Sequence Alignment, Saccharomyces cerevisiae Proteins, Adaptor Proteins, Signal Transducing, Metabolic Engineering

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An internal deletion in MTH1 enables growth on glucose of pyruvate-decarboxylase negative, non-fermentative Saccharomyces cerevisiae

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