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Cited by 141Ion Channels and ReceptorsCalcium signaling and nucleotide metabolismNitric Oxide and Endothelin Effects

TRPV3 regulates nitric oxide synthase-independent nitric oxide synthesis in the skin

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Miyamoto, Takashi, Petrus, Matt J., Dubin, Adrienne E., Patapoutian, Ardem·2011·Nature Communications
DOI 10.1038/ncomms1371PMC PMC3320851
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Abstract

Nitric oxide (NO) is an unstable signalling molecule synthesized de novo mainly from L-arginine by NO synthase (NOS) enzymes. Nitrite reduction can also produce NO, predominantly within body fluids (for example, saliva, sweat and blood plasma) and under extreme hypoxic and acidic conditions. It remains unknown if intracellular canonical signalling pathways regulate nitrite-dependent NO production. Here we examine NO production in the skin, a hypoxic tissue enriched in nitrites wherein NO has important roles in wound healing and other biological processes. We show that activation of TRPV3, a heat-activated transient receptor potential ion channel expressed in keratinocytes, induces NO production via a nitrite-dependent pathway. TRPV3 and nitrite are involved in keratinocyte migration in vitro and in wound healing and thermosensory behaviours in vivo. Our study demonstrates that activation of an ion channel can induce NOS-independent NO production in keratinocytes.

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  • Activation of the TRPV3 channel in the skin can stimulate NO formation independently of NOS, linking nitrite-derived NO to thermoregulation and cutaneous blood flow.

    “For instance, activation of the transient receptor potential vanilloid 3 (TRPV3) channel in the skin can stimulate NO formation independently of NOS, thereby linking nitrite-derived NO to thermoregulation and cutaneous blood flow [39].”

    From Dietary Nitrate-Rich Vegetables as Natural Modulators of Health: Mechanisms and Benefits in Ageing Populations (2026) · cites this paper as [39]

    The Abstract (page unavailable) reports that TRPV3 activation induces nitrite-dependent, NOS-independent NO production in skin keratinocytes. The Results and Discussion link TRPV3 and nitrite to thermosensory behavior, but do not establish a role in thermoregulation or cutaneous blood flow; the Discussion says only that nitrite-dependent NO could participate in heat-induced vasodilation. (LLM, conf=0.87)

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Cited by

Sorted by citations

Authors
Miyamoto, T., Petrus, M. J., Dubin, A. E., & Patapoutian, A.
Affiliations
  • Scripps Research Institute (US)
  • Genomics Institute of the Novartis Research Foundation (US)
Corresponding author
Patapoutian, Ardem
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2011-06-28
Volume
2
Issue
1
Pages
369-369
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/ncomms1371
PMC
PMC3320851
OpenAlex
W1966176614
Cited by
141
References
7
Citations by year
2026: 4 · 2025: 13 · 2024: 9 · 2023: 11 · 2022: 13 · 2021: 11 · 2020: 8 · 2019: 5 · 2018: 6 · 2017: 7 · 2016: 7 · 2015: 15 · 2014: 13 · 2013: 10 · 2012: 8
FWCI
5.52
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
  • Thailand Science Research and Innovation
  • National Institute of Neurological Disorders and Stroke (R01NS046303)
  • National Institute of Dental and Craniofacial Research (R21DE021181)
Topics
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
TRPV3, nitric oxide, nitrite reduction, keratinocytes, skin, wound healing, ion channel activation, hypoxia, keratinocyte migration
MeSH terms
Analysis of Variance, Animals, Animals, Newborn, Cells, Cultured, Nitric Oxide, Nitrites, Wound Healing, Keratinocytes, Polymerase Chain Reaction, DNA Primers, Electroporation, Cell Proliferation, TRPV Cation Channels, Mice

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