The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Identification and microbial production of a terpene-based advanced biofuel
Open accessAbstract
Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l−1. We produce bisabolene in Saccharomyces cerevisiae (>900 mg l−1), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based advanced biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of biofuels. Advanced biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study,Escherichia coliis engineered to produce bisabolene, the immediate precursor of bisabolane, a biosynthetic alternative to D2 diesel.
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Cites
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
Ro, D.-K., Paradise, E. M., Ouellet, M., Fisher, K. J., Newman, K. L., Ndungu, J. M., Ho, K. A., Eachus, R. A., Ham, T. S., Kirby, J., et al. (2006) · Nature · doi:10.1038/nature04640 · Cited by 2,805
Self-citationEngineering Escherichia coli for production of functionalized terpenoids using plant P450s
Chang, M. C. Y., Eachus, R. A., Trieu, W., Ro, D.-K., & Keasling, J. D. (2007) · Nature Chemical Biology · doi:10.1038/nchembio875 · Cited by 364
Self-citationAnthony, J. R., Anthony, L. C., Nowroozi, F., Kwon, G., Newman, J. D., & Keasling, J. D. (2009) · Metabolic Engineering · doi:10.1016/j.ymben.2008.07.007 · Cited by 292
Self-citationRo, D.-K., Ouellet, M., Paradise, E. M., Burd, H., Eng, D., Paddon, C. J., Newman, J. D., & Keasling, J. D. (2008) · BMC Biotechnology · doi:10.1186/1472-6750-8-83 · Cited by 197
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Biofuels for a sustainable future
Liu, Y., Cruz-Morales, P., Zargar, A., Belcher, M. S., Pang, B., Englund, E., Dan, Q., Yin, K., & Keasling, J. D. (2021) · Cell · doi:10.1016/j.cell.2021.01.052 · Cited by 399
Self-citationIntegration of chemical catalysis with extractive fermentation to produce fuels
Anbarasan, P., Baer, Z. C., Sreekumar, S., Gross, E., Binder, J. B., Blanch, H. W., Clark, D. S., & Toste, F. D. (2012) · Nature · doi:10.1038/nature11594 · Cited by 385
The potential of microalgae in biodiesel production
Chen, J., Li, J., Dong, W., Zhang, X., Tyagi, R. D., Drogui, P., & Surampalli, R. Y. (2018) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2018.03.073 · Cited by 330
Chlamydomonas as a model for biofuels and bio‐products production
Scranton, M. A., Ostrand, J. T., Fields, F. J., & Mayfield, S. P. (2015) · The Plant Journal · doi:10.1111/tpj.12780 · Cited by 267
ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in E. coli
Xu, P., Vansiri, A., Bhan, N., & Koffas, M. A. G. (2012) · ACS Synthetic Biology · doi:10.1021/sb300016b · Cited by 255
Metadata
- Authors
- Peralta-Yahya, P. P., Ouellet, M., Chan, R., Mukhopadhyay, A., Keasling, J. D., & Lee, T. S.
- Venue
- Nature Communications
- Published
- 2011-09-27
- Volume
- 2
- Issue
- 1
- Pages
- 483-483
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2138885877
- Cited by
- 637
- References
- 4
- FWCI
- 14.81
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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