The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Oligolysine-based coating protects DNA nanostructures from low-salt denaturation and nuclease degradation
Open accessAbstract
DNA nanostructures have evoked great interest as potential therapeutics and diagnostics due to ease and robustness of programming their shapes, site-specific functionalizations and responsive behaviours. However, their utility in biological fluids can be compromised through denaturation induced by physiological salt concentrations and degradation mediated by nucleases. Here we demonstrate that DNA nanostructures coated by oligolysines to 0.5:1 N:P (ratio of nitrogen in lysine to phosphorus in DNA), are stable in low salt and up to tenfold more resistant to DNase I digestion than when uncoated. Higher N:P ratios can lead to aggregation, but this can be circumvented by coating instead with an oligolysine-PEG copolymer, enabling up to a 1,000-fold protection against digestion by serum nucleases. Oligolysine-PEG-stabilized DNA nanostructures survive uptake into endosomal compartments and, in a mouse model, exhibit a modest increase in pharmacokinetic bioavailability. Thus, oligolysine-PEG is a one-step, structure-independent approach that provides low-cost and effective protection of DNA nanostructures for in vivo applications.
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Cites
Folding DNA to create nanoscale shapes and patterns
Rothemund, P. W. K. (2006) · Nature · doi:10.1038/nature04586 · Cited by 7,354
Boussif, O., Lezoualc'h, F., Zanta, M. A., Mergny, M. D., Scherman, D., Demeneix, B., & Behr, J. P. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7297 · Cited by 6,247
Self-assembly of DNA into nanoscale three-dimensional shapes
Douglas, S. M., Dietz, H., Liedl, T., Högberg, B., Graf, F., & Shih, W. M. (2009) · Nature · doi:10.1038/nature08016 · Cited by 2,571
Self-citationA Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads
Douglas, S. M., Bachelet, I., & Church, G. M. (2012) · Science · doi:10.1126/science.1214081 · Cited by 2,180
Self-assembly of a nanoscale DNA box with a controllable lid
Andersen, E. S., Dong, M., Nielsen, M. M., Jahn, K., Subramani, R., Mamdouh, W., Golas, M. M., Sander, B., Stark, H., Oliveira, C. L. P., et al. (2009) · Nature · doi:10.1038/nature07971 · Cited by 1,627
Folding DNA into Twisted and Curved Nanoscale Shapes
Dietz, H., Douglas, S. M., & Shih, W. M. (2009) · Science · doi:10.1126/science.1174251 · Cited by 1,335
Self-citationThree-Dimensional Structures Self-Assembled from DNA Bricks
Ke, Y., Ong, L. L., Shih, W. M., & Yin, P. (2012) · Science · doi:10.1126/science.1227268 · Cited by 1,246
Self-citationRapid prototyping of 3D DNA-origami shapes with caDNAno
Douglas, S. M., Marblestone, A. H., Teerapittayanon, S., Vazquez, A., Church, G. M., & Shih, W. M. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp436 · Cited by 1,224
Self-citationDNA Origami with Complex Curvatures in Three-Dimensional Space
Han, D., Pal, S., Nangreave, J., Deng, Z., Liu, Y., & Yan, H. (2011) · Science · doi:10.1126/science.1202998 · Cited by 1,214
Jahnen-Dechent, W., & Ketteler, M. (2012) · Clinical Kidney Journal · doi:10.1093/ndtplus/sfr163 · Cited by 1,054
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The current state and future directions of RNAi-based therapeutics
Setten, R. L., Rossi, J. J., & Han, S.-P. (2019) · Nature Reviews Drug Discovery · doi:10.1038/s41573-019-0017-4 · Cited by 1,443
Nuclease resistance of DNA nanostructures
Chandrasekaran, A. R. (2021) · Nature Reviews Chemistry · doi:10.1038/s41570-021-00251-y · Cited by 382
Nanocarrier imaging at single-cell resolution across entire mouse bodies with deep learning
Luo, J., Molbay, M., Chen, Y., Horvath, I., Kadletz, K., Kick, B., Zhao, S., Al-Maskari, R., Singh, I., Ali, M., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-024-02528-1 · Cited by 53
Kohman, R. E., Kunjapur, A. M., Hysolli, E., Wang, Y., & Church, G. M. (2018) · Angewandte Chemie International Edition · doi:10.1002/anie.201707976 · Cited by 33
Metadata
- Authors
- Ponnuswamy, N., Bastings, M. M. C., Nathwani, B., Ryu, J. H., Chou, L. Y. T., Vinther, M., Li, W. A., Anastassacos, F. M., Mooney, D. J., & Shih, W. M.
- Venue
- Nature Communications
- Published
- 2017-05-31
- Volume
- 8
- Issue
- 1
- Pages
- 15654-15654
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2618072306
- Cited by
- 508
- References
- 27
- FWCI
- 16.89
- Type
- journal article
- Language
- en
- Open access
- gold
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- Ponnuswamy, N., Bastings, M. M. C., Nathwani, B., Ryu, J. H., Chou, L. Y. T., Vinther, M., Li, W. A., Anastassacos, F. M., Mooney, D. J., & Shih, W. M. (2017). Oligolysine-based coating protects DNA nanostructures from low-salt denaturation and nuclease degradation. Nature Communications. https://doi.org/10.1038/ncomms15654
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