The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
FTO-mediated formation of N6-hydroxymethyladenosine and N6-formyladenosine in mammalian RNA
Open accessAbstract
N6-methyladenosine is a prevalent internal modification in messenger RNA and non-coding RNA affecting various cellular pathways. Here we report the discovery of two additional modifications, N6-hydroxymethyladenosine (hm6A) and N6-formyladenosine (f6A), in mammalian messenger RNA. We show that FeII- and α-ketoglutarate-dependent fat mass and obesity-associated (FTO) protein oxidize N6-methyladenosine to generate N6-hydroxymethyladenosine as an intermediate modification, and N6-formyladenosine as a further oxidized product. N6-hydroxymethyladenosine and N6-formyladenosine have half-life times of ~3 h in aqueous solution under physiological relevant conditions, and are present in isolated messenger RNA from human cells as well as mouse tissues. These previously unknown modifications derived from the prevalent N6-methyladenosine in messenger RNA, formed through oxidative RNA demethylation, may dynamically modulate RNA–protein interactions to affect gene expression regulation. Internal modifications in mRNA and non-coding RNA are necessary for modulating various intracellular signalling pathways. In this study, the authors report novel modifications resulting from oxidative RNA demethylation, which regulate RNA–protein interactions affecting gene expression.
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Cites
Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons
Meyer, K. D., Saletore, Y., Zumbo, P., Elemento, O., Mason, C. E., & Jaffrey, S. R. (2012) · Cell · doi:10.1016/j.cell.2012.05.003 · Cited by 4,223
N6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO
Jia, G., Fu, Y., Zhao, X., Dai, Q., Zheng, G., Yang, Y., Yi, C., Lindahl, T., Pan, T., Yang, Y.-G., et al. (2011) · Nature Chemical Biology · doi:10.1038/nchembio.687 · Cited by 4,106
Self-citationALKBH5 Is a Mammalian RNA Demethylase that Impacts RNA Metabolism and Mouse Fertility
Zheng, G., Dahl, J. A., Niu, Y., Fedorcsak, P., Huang, C.-M., Li, C. J., Vågbø, C. B., Shi, Y., Wang, W.-L., Song, S.-H., et al. (2013) · Molecular Cell · doi:10.1016/j.molcel.2012.10.015 · Cited by 3,550
Self-citationTet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine
Ito, S., Shen, L., Dai, Q., Wu, S. C., Collins, L. B., Swenberg, J. A., He, C., & Zhang, Y. (2011) · Science · doi:10.1126/science.1210597 · Cited by 3,451
Self-citationThe Nuclear DNA Base 5-Hydroxymethylcytosine Is Present in Purkinje Neurons and the Brain
Kriaucionis, S., & Heintz, N. (2009) · Science · doi:10.1126/science.1169786 · Cited by 2,700
Scuteri, A., Sanna, S., Chen, W.-M., Uda, M., Albai, G., Strait, J., Najjar, S., Nagaraja, R., Orrú, M., Usala, G., et al. (2007) · PLoS Genetics · doi:10.1371/journal.pgen.0030115 · Cited by 1,724
Cited by
m6A modulates neuronal functions and sex determination in Drosophila
Lence, T., Akhtar, J., Bayer, M., Schmid, K., Spindler, L., Ho, C. H., Kreim, N., Andrade-Navarro, M. A., Poeck, B., Helm, M., et al. (2016) · Nature · doi:10.1038/nature20568 · Cited by 544
m 6 A in mRNA: An Ancient Mechanism for Fine-Tuning Gene Expression
Roignant, J.-Y., & Soller, M. (2017) · Trends in Genetics · doi:10.1016/j.tig.2017.04.003 · Cited by 426
The epigenetics of aging and neurodegeneration
Lardenoije, R., Iatrou, A., Kenis, G., Kompotis, K., Steinbusch, H. W. M., Mastroeni, D., Coleman, P., Lemere, C. A., Hof, P. R., van den Hove, D. L. A., et al. (2015) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2015.05.002 · Cited by 381
Mapping the epigenetic modifications of DNA and RNA
Zhao, L.-Y., Song, J., Liu, Y., Song, C.-X., & Yi, C. (2020) · Protein & Cell · doi:10.1007/s13238-020-00733-7 · Cited by 361
Recent advances in dynamic m 6 A RNA modification
Cao, G., Li, H.-B., Yin, Z., & Flavell, R. A. (2016) · Open Biology · doi:10.1098/rsob.160003 · Cited by 308
Metadata
- Authors
- Fu, Y., Jia, G., Pang, X., Wang, R. N., Wang, X., Li, C. J., Smemo, S., Dai, Q., Bailey, K. A., Nobrega, M. A., Han, K.-L., Cui, Q., & He, C.
- Venue
- Nature Communications
- Published
- 2013-04-30
- Volume
- 4
- Issue
- 1
- Pages
- 1798-1798
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2084588717
- Cited by
- 474
- References
- 6
- FWCI
- 8.34
- Type
- journal article
- Language
- en
- Open access
- bronze
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