Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria

Santi, Isabella; Dhar, Neeraj; Bousbaine, Djenet; Wakamoto, Yuichi; McKinney, John D.

doi:10.1038/ncomms3470

Cited by 171Bacterial Genetics and BiotechnologyTuberculosis Research and EpidemiologyGene Regulatory Network Analysis

Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria

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Santi, Isabella, Dhar, Neeraj, Bousbaine, Djenet, Wakamoto, Yuichi, McKinney, John D.·2013·Nature Communications

DOI 10.1038/ncomms3470

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Abstract

During the bacterial cell cycle, chromosome replication and cell division must be coordinated with overall cell growth in order to maintain the correct ploidy and cell size. The spatial and temporal coordination of these processes in mycobacteria is not understood. Here we use microfluidics and time-lapse fluorescence microscopy to measure the dynamics of cell growth, division and chromosome replication in single cells of Mycobacterium smegmatis. We find that single-cell growth is size-dependent (large cells grow faster than small cells), which implicates a size-control mechanism in cell-size homoeostasis. Asymmetric division of mother cells gives rise to unequally sized sibling cells that grow at different velocities but show no differential sensitivity to antibiotics. Individual cells are restricted to one round of chromosome replication per cell division cycle, although replication usually initiates in the mother cell before cytokinesis and terminates in the daughter cells after cytokinesis. These studies reveal important differences between cell cycle organization in mycobacteria compared with better-studied model organisms.

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Mycobacteria divide asymmetrically by a fast, mechanical-snapping mechanism.
“Mycobacteria divide asymmetrically by a fast, mechanical-snapping mechanism (107, 118–121).”
From Mycobacterium smegmatis: The Vanguard of Mycobacterial Research (2023) · cites this paper as [119]
The Discussion reports that mycobacteria usually divide asymmetrically, producing siblings of unequal size. The section on cytokinesis reports a substantial delay between cytokinesis and physical separation, but does not describe separation as a fast, mechanical-snapping mechanism. That mechanism is not supported by this source. (LLM, conf=0.86)
Experimental — accuracy not yet measured
Mycobacteria lack MreB and deposit new cell wall material asymmetrically at the tips of the bacilli.
“Mycobacteria lack MreB and deposit new cell wall material asymmetrically at the tips of the bacilli26,27.”
From The mycobacterial cell envelope — a moving target (2020) · cites this paper as [27]
The Abstract says mycobacteria insert new cell-wall material at their poles, supporting deposition at the tips. But the Discussion reports that old and new poles elongate at the same velocity after cell separation, rather than showing asymmetric deposition between tips. The passages do not establish that mycobacteria lack MreB. (LLM, conf=0.87)
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Authors: Santi, I., Dhar, N., Bousbaine, D., Wakamoto, Y., & McKinney, J. D.
Affiliations: École Polytechnique Fédérale de Lausanne (CH)
The University of Tokyo (JP)
Corresponding author: Santi, Isabella
ORCID: Santi, Isabella
Dhar, Neeraj
Bousbaine, Djenet
Wakamoto, Yuichi
McKinney, John D.
Venue: Nature Communications
Publisher: Springer Science and Business Media LLC
Published: 2013-09-16
Volume: 4
Issue: 1
Pages: 2470-2470
ISSN: 2041-1723 (ISSN-L)
DOI: 10.1038/ncomms3470
OpenAlex: W2018825030
Cited by: 170
References: 39
Citations by year: 2026: 2 · 2025: 9 · 2024: 9 · 2023: 13 · 2022: 9 · 2021: 11 · 2020: 12 · 2019: 17 · 2018: 26 · 2017: 23 · 2016: 10 · 2015: 15 · 2014: 13
FWCI: 8.38
Type: journal article
Language: en
Update notices: erratum (2013-12-03)
Open access: bronze
doi.org
Licence: www.springernature.com/gp/researchers/text-and-data-mining (tdm)
www.springernature.com/gp/researchers/text-and-data-mining (vor)
Locations: Nature Communications (publishedVersion)
PubMed (publishedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Funders: National Science Foundation
Bill and Melinda Gates Foundation
Ministry of Education, Culture, Sports, Science and Technology
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (135639)
National Institutes of Health
Japan Society for the Promotion of Science (25711008)
National Heart, Lung, and Blood Institute (HL088906, R01HL088906)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
Keywords: bacterial cell cycle, chromosome replication, cell division, Mycobacterium smegmatis, cell size homeostasis, size-dependent growth, asymmetric cell division, single-cell dynamics, cytokinesis, antibiotic susceptibility, microfluidics, time-lapse fluorescence microscopy
MeSH terms: Anti-Bacterial Agents, Bacterial Proteins, Cell Division, Chromosomes, Bacterial, DNA Replication, Homeostasis, Mycobacterium smegmatis, Protein Transport, Cell Size, Cytokinesis, Cell Proliferation, Green Fluorescent Proteins, Time-Lapse Imaging, Single-Cell Analysis

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