Subpolar addition of new cell wall is directed by DivIVA in mycobacteria
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Significance: The tropomyosin-like protein, DivIVA, determines the site of growth and cell morphology in mycobacteria. Surprisingly, although DivIVA is located at the tip of the growing cell pole, cell wall addition is excluded from this site. Both late cell wall synthetic enzymes and new cell wall deposition occur at a subpolar space, distinct from the DivIVA-marked cell tip. Instead of directly recruiting terminal cell wall synthetic systems, DivIVA interacts with enzymes involved in the early steps of the cell wall precursor synthesis. These results suggest a unique organization of the polar elongasome, where cell wall precursors are concentrated at the cell tip by DivIVA and then incorporated into the nascent cell wall in an annular pattern at the subpolar zone.
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DivIVA is localized to the poles where it has been shown to promote peptidoglycan biosynthesis and cell elongation in M. smegmatis.
“DivIVA is localized to the poles where it has been shown to promote peptidoglycan biosynthesis and cell elongation in M. smegmatis (108–111).”
From Mycobacterium smegmatis: The Vanguard of Mycobacterial Research (2023) · cites this paper as [109]
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BackgroundSelf-citation
- Authors
- Meniche, X., Otten, R., Siegrist, M. S., Baer, C. E., Murphy, K. C., Bertozzi, C. R., & Sassetti, C. M.
- Affiliations
- Department of Microbiology and Physiological Systems and
- Brandeis University (US)
- University of California, Berkeley (US)
- Howard Hughes Medical Institute (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2014-07-21
- Volume
- 111
- Issue
- 31
- Pages
- E3243-51
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4128124
- OpenAlex
- W2041353321
- Cited by
- 175
- References
- 55
- Citations by year
- 2026: 10 · 2025: 12 · 2024: 8 · 2023: 14 · 2022: 10 · 2021: 17 · 2020: 21 · 2019: 15 · 2018: 24 · 2017: 15 · 2016: 13 · 2015: 12 · 2014: 4
- FWCI
- 10.31
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- Damon Runyon Cancer Research Foundation (DRG-2114-12)
- National Institutes of Health (AI051622)
- National Institute of Allergy and Infectious Diseases (AI10954208, R01AI051622, AI051622, R01AI064282, R37AI051622)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Keywords
- mycobacteria, DivIVA, polar growth, cell wall biosynthesis, peptidoglycan biosynthesis, arabinogalactan, mycolic acids, cell shape, cell division
- MeSH terms
- Bacterial Proteins, Cell Membrane, Cell Wall, Models, Biological, Mycolic Acids, Protein Binding, Cell Polarity, Cell Cycle Proteins, Mycobacterium smegmatis
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