The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio
Open accessAbstract
The ATP:ADP ratio is a critical parameter of cellular energy status that regulates many metabolic activities. Here we report an optimized genetically encoded fluorescent biosensor, PercevalHR, that senses the ATP:ADP ratio. PercevalHR is tuned to the range of intracellular ATP:ADP expected in mammalian cells, and it can be used with one- or two-photon microscopy in live samples. We use PercevalHR to visualize activity-dependent changes in ATP:ADP when neurons are exposed to multiple stimuli, demonstrating that it is a sensitive reporter of physiological changes in energy consumption and production. We also use PercevalHR to visualize intracellular ATP:ADP while simultaneously recording currents from ATP-sensitive potassium (KATP) channels in single cells, showing that PercevalHR enables the study of coordinated variation in ATP:ADP and KATP channel open probability in intact cells. With its ability to monitor changes in cellular energetics within seconds, PercevalHR should be a versatile tool for metabolic research. The ratio between ATP and ADP within the cell is a key indicator of metabolic status. Tantama et al. describe a ratiometric, genetically encoded fluorescent sensor for ATP:ADP that is now optimized for mammalian cells, and demonstrate that it can detect physiological changes in energy consumption and production.
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Cites
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Imaging cytosolic NADH-NAD(+) redox state with a genetically encoded fluorescent biosensor
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Understanding metabolic regulation and its influence on cell physiology
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Activity-driven local ATP synthesis is required for synaptic function
Rangaraju, V., Calloway, N., & Ryan, T. A. (2014) · Cell · doi:10.1016/j.cell.2013.12.042 · Cited by 836
Labeling of active neural circuits in vivo with designed calcium integrators
Fosque, B. F., Sun, Y., Dana, H., Yang, C.-T., Ohyama, T., Tadross, M. R., Patel, R., Zlatic, M., Kim, D. S., Ahrens, M. B., et al. (2015) · Science · doi:10.1126/science.1260922 · Cited by 458
Yaginuma, H., Kawai, S., Tabata, K. V., Tomiyama, K., Kakizuka, A., Komatsuzaki, T., Noji, H., & Imamura, H. (2014) · Scientific Reports · doi:10.1038/srep06522 · Cited by 386
Energy matters: presynaptic metabolism and the maintenance of synaptic transmission
Li, S., & Sheng, Z.-H. (2022) · Nature Reviews Neuroscience · doi:10.1038/s41583-021-00535-8 · Cited by 267
Zanotelli, M. R., Rahman-Zaman, A., VanderBurgh, J. A., Taufalele, P. V., Jain, A., Erickson, D., Bordeleau, F., & Reinhart-King, C. A. (2019) · Nature Communications · doi:10.1038/s41467-019-12155-z · Cited by 165
Metadata
- Authors
- Tantama, M., Martínez-François, J. R., Mongeon, R., & Yellen, G.
- Venue
- Nature Communications
- Published
- 2013-10-07
- Volume
- 4
- Issue
- 1
- Pages
- 2550-2550
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2064005757
- Cited by
- 483
- References
- 9
- FWCI
- 11.20
- Type
- journal article
- Language
- en
- Open access
- bronze
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