The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Visualization and targeted disruption of protein interactions in living cells
Open accessAbstract
Protein–protein interactions are the basis of all processes in living cells, but most studies of these interactions rely on biochemical in vitro assays. Here we present a simple and versatile fluorescent-three-hybrid (F3H) strategy to visualize and target protein–protein interactions. A high-affinity nanobody anchors a GFP-fusion protein of interest at a defined cellular structure and the enrichment of red-labelled interacting proteins is measured at these sites. With this approach, we visualize the p53–HDM2 interaction in living cells and directly monitor the disruption of this interaction by Nutlin 3, a drug developed to boost p53 activity in cancer therapy. We further use this approach to develop a cell-permeable vector that releases a highly specific peptide disrupting the p53 and HDM2 interaction. The availability of multiple anchor sites and the simple optical readout of this nanobody-based capture assay enable systematic and versatile analyses of protein–protein interactions in practically any cell type and species. Screens for protein–protein interactions and for drugs that disrupt them typically use in vitro assays which fail to capture the complexity of the cell’s interior. By fixing proteins to distinct cellular locations, Herce et al.demonstrate a fluorescent-three-hybrid approach to probe such interactions in their cellular contexts.
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Cites
In Vivo Activation of the p53 Pathway by Small-Molecule Antagonists of MDM2
Vassilev, L. T., Vu, B. T., Graves, B., Carvajal, D., Podlaski, F., Filipovic, Z., Kong, N., Kammlott, U., Lukacs, C., Klein, C., et al. (2004) · Science · doi:10.1126/science.1092472 · Cited by 4,661
Amplification of a gene encoding a p53-associated protein in human sarcomas
Oliner, J. D., Kinzler, K. W., Meltzer, P. S., George, D. L., & Vogelstein, B. (1992) · Nature · doi:10.1038/358080a0 · Cited by 1,949
Small-molecule inhibitors of protein–protein interactions: progressing towards the dream
Arkin, M. R., & Wells, J. A. (2004) · Nature Reviews Drug Discovery · doi:10.1038/nrd1343 · Cited by 1,705
Rescue of early embryonic lethality in mdm2-deficient mice by deletion of p53
de Oca Luna, R. M., Wagner, D. S., & Lozano, G. (1995) · Nature · doi:10.1038/378203a0 · Cited by 1,420
Rescue of embryonic lethality in Mdm2-deficient mice by absence of p53
Jones, S. N., Roe, A. E., Donehower, L. A., & Bradley, A. (1995) · Nature · doi:10.1038/378206a0 · Cited by 1,280
STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES
Scherer, W. F., Syverton, J. T., & Gey, G. O. (1953) · Journal of Experimental Medicine · doi:10.1084/jem.97.5.695 · Cited by 856
Translating p53 into the clinic
Cheok, C. F., Verma, C. S., Baselga, J., & Lane, D. P. (2011) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2010.174 · Cited by 381
Comparative study of the p53-mdm2 and p53-MDMX interfaces
Böttger, V., Böttger, A., Garcia-Echeverria, C., Ramos, Y. F., van der Eb, A. J., Jochemsen, A. G., & Lane, D. P. (1999) · Oncogene · doi:10.1038/sj.onc.1202281 · Cited by 189
Cited by
Kristensen, M., Birch, D., & Mørck Nielsen, H. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17020185 · Cited by 304
Cell-permeable nanobodies for targeted immunolabelling and antigen manipulation in living cells
Herce, H. D., Schumacher, D., Schneider, A. F. L., Ludwig, A. K., Mann, F. A., Fillies, M., Kasper, M.-A., Reinke, S., Krause, E., Leonhardt, H., et al. (2017) · Nature Chemistry · doi:10.1038/nchem.2811 · Cited by 293
Self-citationNischan, N., Herce, H. D., Natale, F., Bohlke, N., Budisa, N., Cardoso, M. C., & Hackenberger, C. P. R. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201410006 · Cited by 287
Self-citationFerry, L., Fournier, A., Tsusaka, T., Adelmant, G., Shimazu, T., Matano, S., Kirsh, O., Amouroux, R., Dohmae, N., Suzuki, T., et al. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.07.012 · Cited by 203
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Metadata
- Authors
- Herce, H. D., Deng, W., Helma, J., Leonhardt, H., & Cardoso, M. C.
- Venue
- Nature Communications
- Published
- 2013-10-24
- Volume
- 4
- Issue
- 1
- Pages
- 2660-2660
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2122013271
- Cited by
- 162
- References
- 8
- FWCI
- 6.45
- Type
- journal article
- Language
- en
- Open access
- hybrid
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