STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES
Open accessAbstract
The cells of a human epithelial cancer cultivated en masse have been shown to support the multiplication of all three types of poliomyelitis virus. These cells (strain HeLa of Gey) have been maintained in vitro since their derivation from an epidermoid carcinoma of the cervix in February, 1951. As the virus multiplied it caused in from 12 to 96 hours degeneration and destruction of the cancer cells. The specific destructive effect of the virus was prevented by adding homotypic antibody to the cultures but not by adding heterotypic antibodies. Methods for the preparation of large numbers of replicate cultures with suspensions of strain HeLa cells were described. The cells in suspension were readily quantitated by direct counts in a hemocytometer. A synthetic solution that maintains cellular viability was employed for viral propagation. The experimental results demonstrate the usefulness of strain HeLa cells for (a) the quantitation of poliomyelitis virus, (b) the measurement of poliomyelitis antibodies, and (c) the production of virus.
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The HeLa cells used were authenticated and tested for mycoplasma.
“Authentic and mycoplasma-tested HeLa cells (Scherer et al., 1953) were maintained as semiconfluent monolayers in DMEM”
From Distinct anterograde trafficking pathways of BACE1 and amyloid precursor protein from the TGN and the regulation of amyloid-β production (2020) · cites this paper as Scherer et al., 1953
The Cell Strain passage identifies the origin and cultivation of HeLa cells, so the citation supports the cell-line identification. It does not report authentication or mycoplasma testing of the cells used in the citing study. (LLM, conf=0.87)
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- Authors
- Scherer, W. F., Syverton, J. T., & Gey, G. O.
- Affiliations
- University of Minnesota (US)
- Venue
- Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 1953-05-01
- Volume
- 97
- Issue
- 5
- Pages
- 695-710
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- PMC
- PMC2136303
- OpenAlex
- W162187343
- Cited by
- 856
- References
- 0
- Citations by year
- 2026: 19 · 2025: 16 · 2024: 29 · 2023: 25 · 2022: 33 · 2021: 35 · 2020: 36 · 2019: 46 · 2018: 27 · 2017: 33 · 2016: 30 · 2015: 40 · 2014: 42 · 2013: 31 · 2012: 38
- FWCI
- 61.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Experimental Medicine (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- American Cancer Society
- National Institutes of Health
- National Cancer Institute
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- poliovirus, HeLa cells, heterotypic antibodies, virus quantitation, virus production, cell suspension culture
- MeSH terms
- Antibodies, Antibodies, Heterophile, Carcinoma, Squamous Cell, Cervix Uteri, Female, HeLa Cells, Humans, Poliomyelitis, Viruses, Poliovirus, Tissue Culture Techniques, In Vitro Techniques
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