The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability
Abstract
The delivery of free molecules into the cytoplasm and nucleus by using arginine‐rich cell‐penetrating peptides (CPPs) has been limited to small cargoes, while large cargoes such as proteins are taken up and trapped in endocytic vesicles. Based on recent work, in which we showed that the transduction efficiency of arginine‐rich CPPs can be greatly enhanced by cyclization, the aim was to use cyclic CPPs to transport full‐length proteins, in this study green fluorescent protein (GFP), into the cytosol of living cells. Cyclic and linear CPP–GFP conjugates were obtained by using azido‐functionalized CPPs and an alkyne‐functionalized GFP. Our findings reveal that the cyclic‐CPP–GFP conjugates are internalized into live cells with immediate bioavailability in the cytosol and the nucleus, whereas linear CPP analogues do not confer GFP transduction. This technology expands the application of cyclic CPPs to the efficient transport of functional full‐length proteins into live cells.
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Cites
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Cited by
Liu, C., Wan, T., Wang, H., Zhang, S., Ping, Y., & Cheng, Y. (2019) · Science Advances · doi:10.1126/sciadv.aaw8922 · Cited by 396
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The fluorination effect of fluoroamphiphiles in cytosolic protein delivery
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Cell-permeable nanobodies for targeted immunolabelling and antigen manipulation in living cells
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Metadata
- Authors
- Nischan, N., Herce, H. D., Natale, F., Bohlke, N., Budisa, N., Cardoso, M. C., & Hackenberger, C. P. R.
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2014-12-17
- Volume
- 54
- Issue
- 6
- Pages
- 1950-1953
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W2109741979
- Cited by
- 287
- References
- 31
- FWCI
- 6.33
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nischan, N., Herce, H. D., Natale, F., Bohlke, N., Budisa, N., Cardoso, M. C., & Hackenberger, C. P. R. (2015). Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability. Angewandte Chemie International Edition. https://doi.org/10.1002/anie.201410006
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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