The DOI matches its Crossref record; 68 of the 68 references it cites were found in a registry or an index.
Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue
Open accessAbstract
In the developing neural retina (NR), multipotent stem cells within the ciliary margin (CM) contribute to de novo retinal tissue growth. We recently reported the ability of human embryonic stem cells (hESCs) to self-organize stratified NR using a three-dimensional culture technique. Here we report the emergence of CM-like stem cell niches within human retinal tissue. First, we developed a culture method for selective NR differentiation by timed BMP4 treatment. We then found that inhibiting GSK3 and FGFR induced the transition from NR tissue to retinal pigment epithelium (RPE), and that removing this inhibition facilitated the reversion of this RPE-like tissue back to the NR fate. This step-wise induction-reversal method generated tissue aggregates with RPE at the margin of central-peripherally polarized NR. We demonstrate that the NR–RPE boundary tissue further self-organizes a niche for CM stem cells that functions to expand the NR peripherally by de novo progenitor generation. The ciliary margin of the eye functions as a source of multipotent progenitor cells in certain organisms but whether it plays this role in humans has not been easy to study. Here the authors culture human embryonic stem cells that self-organize into retinal tissue, and show that ciliary margin-like growth zones emerge from the developing human retinal tissue and contain stem cell niches.
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Capowski, E. E., Samimi, K., Mayerl, S. J., Phillips, M. J., Pinilla, I., Howden, S. E., Saha, J., Jansen, A. D., Edwards, K. L., Jager, L. D., et al. (2018) · Development · doi:10.1242/dev.171686 · Cited by 311
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Metadata
- Authors
- Kuwahara, A., Ozone, C., Nakano, T., Saito, K., Eiraku, M., & Sasai, Y.
- Venue
- Nature Communications
- Published
- 2015-02-19
- Volume
- 6
- Issue
- 1
- Pages
- 6286-6286
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2063288238
- Cited by
- 349
- References
- 68
- FWCI
- 9.52
- Type
- journal article
- Language
- en
- Open access
- gold
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- Kuwahara, A., Ozone, C., Nakano, T., Saito, K., Eiraku, M., & Sasai, Y. (2015). Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue. Nature Communications. https://doi.org/10.1038/ncomms7286
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