The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Targeted DNA degradation using a CRISPR device stably carried in the host genome
Open accessAbstract
Once an engineered organism completes its task, it is useful to degrade the associated DNA to reduce environmental release and protect intellectual property. Here we present a genetically encoded device (DNAi) that responds to a transcriptional input and degrades user-defined DNA. This enables engineered regions to be obscured when the cell enters a new environment. DNAi is based on type-IE CRISPR biochemistry and a synthetic CRISPR array defines the DNA target(s). When the input is on, plasmid DNA is degraded 10 8 -fold. When the genome is targeted, this causes cell death, reducing viable cells by a factor of 10 8 . Further, the CRISPR nuclease can direct degradation to specific genomic regions (for example, engineered or inserted DNA), which could be used to complicate recovery and sequencing efforts. DNAi can be stably carried in an engineered organism, with no impact on cell growth, plasmid stability or DNAi inducibility even after passaging for >2 months.
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RNA-Guided Human Genome Engineering via Cas9
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Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
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CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III
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RNA-guided editing of bacterial genomes using CRISPR-Cas systems
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Permanent genetic memory with >1-byte capacity
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Self-citationSalmonella enterica Serovar Typhimurium Strains with Regulated Delayed Attenuation In Vivo
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Engineering Microbial Living Therapeutics: The Synthetic Biology Toolbox
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Structural basis for promiscuous PAM recognition in type I–E Cascade from E. coli
Hayes, R. P., Xiao, Y., Ding, F., van Erp, P. B. G., Rajashankar, K., Bailey, S., Wiedenheft, B., & Ke, A. (2016) · Nature · doi:10.1038/nature16995 · Cited by 187
Current status and perspectives of genome editing technology for microalgae
Jeon, S., Lim, J.-M., Lee, H.-G., Shin, S.-E., Kang, N. K., Park, Y.-I., Oh, H.-M., Jeong, W.-J., Jeong, B.-R., & Chang, Y. K. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0957-z · Cited by 154
Metadata
- Authors
- Caliando, B. J., & Voigt, C. A.
- Venue
- Nature Communications
- Published
- 2015-05-19
- Volume
- 6
- Issue
- 1
- Pages
- 6989-6989
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2175214920
- Cited by
- 173
- References
- 8
- FWCI
- 6.05
- Type
- journal article
- Language
- en
- Open access
- gold
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