The forkhead protein Foxj1 specifies node-like cilia in Xenopus and zebrafish embryos
Open accessAbstract
It has been proposed that ciliated cells that produce a leftward fluid flow mediate left-right patterning in many vertebrate embryos. The cilia on these cells combine features of primary sensory and motile cilia, but how this cilia subtype is specified is unknown. We address this issue by analyzing the Xenopus and zebrafish homologs of Foxj1, a forkhead transcription factor necessary for ciliogenesis in multiciliated cells of the mouse. We show that the cilia that underlie left-right patterning on the Xenopus gastrocoel roof plate (GRP) and zebrafish Kupffer's vesicle are severely shortened or fail to form in Foxj1 morphants. We also show that misexpressing Foxj1 is sufficient to induce ectopic GRP-like cilia formation in frog embryos. Microarray analysis indicates that Xenopus Foxj1 induces the formation of cilia by upregulating the expression of motile cilia genes. These results indicate that Foxj1 is a critical determinant in the specification of cilia used in left-right patterning.
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The evolutionary divergence of mucociliary cell types in metazoans appears closely linked to the diversification of the Fox gene family.
“At the molecular level, the evolutionary divergence of mucociliary cell types in metazoans appears to be closely linked to the diversification of the Fox gene family.”
From Gastric pouches and the mucociliary sole: setting the stage for nervous system evolution (2015) · cites this paper as [112]
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- Authors
- Stubbs, J. L., Oishi, I., Izpisúa Belmonte, J. C., & Kintner, C.
- Affiliations
- Salk Institute for Biological Studies (US)
- Corresponding author
- Kintner, Chris
- Venue
- Nature Genetics
- Published
- 2008-11-16
- Volume
- 40
- Issue
- 12
- Pages
- 1454-1460
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- PMC
- PMC4648715
- OpenAlex
- W2106974809
- Cited by
- 327
- References
- 45
- Citations by year
- 2026: 8 · 2025: 14 · 2024: 28 · 2023: 22 · 2022: 17 · 2021: 15 · 2020: 15 · 2019: 17 · 2018: 19 · 2017: 22 · 2016: 20 · 2015: 25 · 2014: 33 · 2013: 15 · 2012: 12
- FWCI
- 8.55
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Genetics (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Foundation for the National Institutes of Health
- Fundación Cellex
- G. Harold and Leila Y. Mathers Foundation
- Ministerio de Economía y Competitividad
- Kanae Foundation for the Promotion of Medical Science
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM076507)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- Foxj1, motile cilia, left-right asymmetry, ciliogenesis, Kupffer's vesicle, primary cilia, transcription factors, Xenopus, zebrafish
- MeSH terms
- Animals, Cilia, Xenopus, Zebrafish, Body Patterning, Xenopus Proteins, Zebrafish Proteins, Forkhead Transcription Factors
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