Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos
Abstract
Mesoderm formation is critical for the establishment of the animal body plan and in Drosophila requires the snail gene. This report concerns the cloning and expression pattern of the structurally similar gene snail1 from zebrafish. In situ hybridization shows that the quantity of snail1 RNA increases at the margin of the blastoderm in cells that involute during gastrulation. As gastrulation begins, snail1 RNA disappears from the dorsal axial mesoderm and becomes restricted to the paraxial mesoderm and the tail bud. snail1 RNA increases in cells that define the posterior border of each somite and then disappears when somitic cells differentiate. Later in development, expression appears in cephalic neural crest derivatives. Many snail1-expressing cells were missing from mutant spadetail embryos and the quantity of snail1 RNA was greatly reduced in mutant no tail embryos. The work presented here suggests that snail1 is involved in morphogenetic events during gastrulation, somitogenesis and development of the cephalic neural crest, and that no tail may act as a positive regulator of snail1.
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Zebrafish Snail1 homolog Snail1a is required for EMT and neural crest development.
“As the zebrafish Snail1 homolog Snail1a is also required for EMT and neural crest development (58), this model was more amenable to the use of siRNA silencing to globally knock down expression of Snail1a.”
From Bacterial induction of Snail1 contributes to blood-brain barrier disruption (2015) · cites this paper as [58]
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- Authors
- Thisse, C., Thisse, B., Schilling, T. F., & Postlethwait, J. H.
- Affiliations
- University of Oregon (US)
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1993-12-01
- Volume
- 119
- Issue
- 4
- Pages
- 1203-1215
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W1952870715
- Cited by
- 877
- References
- 49
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 8 · 2023: 12 · 2022: 11 · 2021: 16 · 2020: 18 · 2019: 19 · 2018: 21 · 2017: 16 · 2016: 21 · 2015: 20 · 2014: 27 · 2013: 32 · 2012: 39
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ai026734-02)
- National Institute of Allergy and Infectious Diseases (1RO1AI26734)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (P01HD022486, 1PO1HD-22486, 1T32HDO7348, T32HD007348)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Keywords
- SNAI1, zebrafish, mesoderm formation, gastrulation, somitogenesis, cranial neural crest, gene expression patterns, tail bud, body plan establishment, transcriptional regulation
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Gastrula, Genes, Mesoderm, Molecular Sequence Data, Morphogenesis, Mutation, Neural Crest, Zebrafish, Gene Expression, Sequence Homology, In Situ Hybridization
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