The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Interaction between differentially methylated regions partitions the imprinted genes Igf2 and H19 into parent-specific chromatin loops
Abstract
Imprinted genes are expressed from only one of the parental alleles and are marked epigenetically by DNA methylation and histone modifications. The paternally expressed gene insulin-like growth-factor 2 (Igf2) is separated by approximately 100 kb from the maternally expressed noncoding gene H19 on mouse distal chromosome 7. Differentially methylated regions in Igf2 and H19 contain chromatin boundaries, silencers and activators and regulate the reciprocal expression of the two genes in a methylation-sensitive manner by allowing them exclusive access to a shared set of enhancers. Various chromatin models have been proposed that separate Igf2 and H19 into active and silent domains. Here we used a GAL4 knock-in approach as well as the chromosome conformation capture technique to show that the differentially methylated regions in the imprinted genes Igf2 and H19 interact in mice. These interactions are epigenetically regulated and partition maternal and paternal chromatin into distinct loops. This generates a simple epigenetic switch for Igf2 through which it moves between an active and a silent chromatin domain.
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“An insulated neighborhood on the maternal allele allows an enhancer-promoter interaction that activates the H19 gene but not the IGF2 gene, which is excluded from the neighborhood.”
From Insulated Neighborhoods: Structural and Functional Units of Mammalian Gene Control (2016) · cites this paper as Murrell et al., 2004
In the citing paper
“Parent-of-origin specific gene activity at the imprinted IGF2/H19 locus is controlled by allele-specific CTCF-CTCF interactions that constrain enhancer-gene contacts in a DNA methylation-dependent manner (Figure 6A) (Kurukuti et al., 2006; Murrell et al., 2004).”
From Insulated Neighborhoods: Structural and Functional Units of Mammalian Gene Control (2016) · cites this paper as Murrell et al., 2004
In the citing paper
“A larger insulated neighborhood is formed on the paternal allele to allow an enhancer-promoter interaction that activates the IGF2 gene.”
From Insulated Neighborhoods: Structural and Functional Units of Mammalian Gene Control (2016) · cites this paper as Murrell et al., 2004
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“Chromatin loops may also contribute to gene silencing, as looping of imprinting-specific regions occurs parent specifically in the insulin-like growth factor 2, H19 gene cluster (Murrell et al., 2004), and the maternally expressed DLX5 locus (Horike et al.”
Metadata
- Authors
- Murrell, A., Heeson, S., & Reik, W.
- Affiliations
- Babraham Institute (GB)
- Corresponding author
- Murrell, Adele
- Reik, Wolf
- Venue
- Nature Genetics
- Published
- 2004-07-25
- Volume
- 36
- Issue
- 8
- Pages
- 889-893
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- OpenAlex
- W2085509761
- Cited by
- 556
- References
- 30
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 13 · 2023: 5 · 2022: 8 · 2021: 8 · 2020: 10 · 2019: 13 · 2018: 16 · 2017: 15 · 2016: 18 · 2015: 26 · 2014: 27 · 2013: 30 · 2012: 50
- FWCI
- 22.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Syndromes and Imprinting
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- genomic imprinting, insulin-like growth factor II, H19, differentially methylated regions, chromatin looping, DNA methylation, epigenetic regulation, chromosome conformation, epigenetic switch, mouse chromosome 7, gene silencing
- MeSH terms
- Animals, Chromatin, Insulin-Like Growth Factor II, Mice, Transgenic, Molecular Sequence Data, Genomic Imprinting, DNA Methylation, RNA, Untranslated, Mice, RNA, Long Noncoding
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