Cited by 502Genomics and Chromatin DynamicsEpigenetics and DNA MethylationCancer-related gene regulation

Histone H3 lysine 9 methylation is an epigenetic imprint of facultative heterochromatin

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Peters, Antoine H.F.M., Mermoud, Jacqueline E., O'Carroll, Dónal, Pagani, Michaela, Schweizer, Dieter, Brockdorff, Neil, Jenuwein, Thomas·2002·Nature Genetics

Abstract

Post-translational modifications of histone amino termini are an important regulatory mechanism that induce transitions in chromatin structure, thereby contributing to epigenetic gene control and the assembly of specialized chromosomal subdomains. Methylation of histone H3 at lysine 9 (H3-Lys9) by site-specific histone methyltransferases (Suv39h HMTases) marks constitutive heterochromatin. Here, we show that H3-Lys9 methylation also occurs in facultative heterochromatin of the inactive X chromosome (Xi) in female mammals. H3-Lys9 methylation is retained through mitosis, indicating that it might provide an epigenetic imprint for the maintenance of the inactive state. Disruption of the two mouse Suv39h HMTases abolishes H3-Lys9 methylation of constitutive heterochromatin but not that of the Xi. In addition, HP1 proteins, which normally associate with heterochromatin, do not accumulate with the Xi. These observations suggest the existence of an Suv39h-HP1-independent pathway regulating H3-Lys9 methylation of facultative heterochromatin.

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Crossref · 10.1038/ng789

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Authors
Peters, A. H. F. M., Mermoud, J. E., O'Carroll, D., Pagani, M., Schweizer, D., Brockdorff, N., & Jenuwein, T.
Affiliations
  • Research Institute of Molecular Pathology (AT)
  • Hammersmith Hospital (GB)
  • University of Vienna (AT)
Corresponding author
Jenuwein, Thomas
Venue
Nature Genetics
Published
2001-12-10
Volume
30
Issue
1
Pages
77-80
ISSN
  • 1061-4036 (ISSN-L)
  • 1546-1718
OpenAlex
W2060863780
Cited by
502
References
30
Citations by year
2026: 3 · 2025: 5 · 2024: 10 · 2023: 9 · 2022: 12 · 2021: 15 · 2020: 9 · 2019: 12 · 2018: 10 · 2017: 23 · 2016: 16 · 2015: 18 · 2014: 21 · 2013: 11 · 2012: 20
FWCI
14.66
Type
journal article
Language
en
Open access
Locations
  • Nature Genetics (publishedVersion)
  • PubMed (publishedVersion)
  • Newcastle University ePrints (Newcastle Univesity)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer-related gene regulation
Keywords
histone H3 lysine 9 methylation, facultative heterochromatin, inactive X chromosome, Suv39h, histone methyltransferase, HP1 proteins, constitutive heterochromatin, chromatin structure, X chromosome inactivation
MeSH terms
Histone Methyltransferases, Amniocentesis, Aneuploidy, Animals, Binding Sites, Cells, Cultured, Chromosomes, Human, Pair 4, Dosage Compensation, Genetic, Female, Fibroblasts, Gene Expression Regulation, Heterochromatin, Histones, Humans, Lysine, Male, Metaphase, Methylation, Methyltransferases, Microscopy, Fluorescence, Mouth Mucosa, Precipitin Tests, Pregnancy, Protein Binding, Histone-Lysine N-Methyltransferase, Protein Methyltransferases, Repressor Proteins, Translocation, Genetic, X Chromosome, Protein Isoforms, Chromosome Painting, Mice

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