Cited by 213Genomics and Chromatin DynamicsEpigenetics and DNA MethylationChromosomal and Genetic Variations

Position-effect variegation and the genetic dissection of chromatin regulation in Drosophila

Schotta, Gunnar, Ebert, Anja, Dorn, Rainer, Reuter, Gunter·2003·Seminars in Cell & Developmental Biology

Abstract

In position-effect variegation (PEV) genes become silenced by heterochromatisation. Genetic dissection of this process has been performed by means of dominant suppressor [Su(var)] and enhancer [E(var)] mutations. Selective genetic screens allowed mass isolation of more than 380 PEV modifier mutations identifying about 150 genes. Genetic fine structure studies revealed unique dosage dependent effects. Most of the haplo-dependent Su(var) and E(var) genes do not display triplo-dependent effects. Several Su(var) loci with triplo-dependent opposite enhancer effects have been identified and shown to encode heterochromatin-associated proteins. From these the evolutionary conserved histone H3 lysine 9 methyltransferase SU(VAR)3-9 plays a central role in heterochromatic gene silencing. Molecular function of most PEV modifier genes is still unknown also including genes identified with mutations displaying lethal interaction to heterochromatin. Their analysis should contribute to further understanding of processes connected with regulation of higher order chromatin structure and epigenetic programming.

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Crossref · 10.1016/s1084-9521(02)00138-6

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Authors
Schotta, G., Ebert, A., Dorn, R., & Reuter, G.
Affiliations
Martin Luther University Halle-Wittenberg (DE)
Venue
Seminars in Cell & Developmental Biology
Publisher
Elsevier BV
Published
2003-02
Volume
14
Issue
1
Pages
67-75
ISSN
1084-9521 (ISSN-L)
OpenAlex
W2053291659
Cited by
213
References
62
Citations by year
2026: 2 · 2025: 5 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 5 · 2019: 6 · 2017: 5 · 2016: 5 · 2015: 6 · 2014: 14 · 2013: 14 · 2012: 5
FWCI
1.79
Type
journal article
Language
en
Open access
closed
Locations
  • Seminars in Cell and Developmental Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
Keywords
position effect variegation, heterochromatin, gene silencing, dose-dependent effects, Su(var)3-9, histone H3 lysine 9 methylation, chromatin regulation, epigenetic regulation, Drosophila
MeSH terms
Animals, Chromatin, Drosophila, Heterochromatin, Methyltransferases, Mutation, Phenotype, Gene Silencing

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