The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization
Open accessAbstract
The angiotensin-converting enzyme (ACE) is a key regulator of blood pressure. It is known to cleave small peptides, such as angiotensin I and bradykinin and changes their biological activities, leading to upregulation of blood pressure. Here we describe a new activity for ACE: a glycosylphosphatidylinositol (GPI)-anchored protein releasing activity (GPIase activity). Unlike its peptidase activity, GPIase activity is weakly inhibited by the tightly binding ACE inhibitor and not inactivated by substitutions of core amino acid residues for the peptidase activity, suggesting that the active site elements for GPIase differ from those for peptidase activity. ACE shed various GPI-anchored proteins from the cell surface, and the process was accelerated by the lipid raft disruptor filipin. The released products carried portions of the GPI anchor, indicating cleavage within the GPI moiety. Further analysis by high-performance liquid chromatography–mass spectrometry predicted the cleavage site at the mannose-mannose linkage. GPI-anchored proteins such as TESP5 and PH-20 were released from the sperm membrane of wild-type mice but not in Ace knockout sperm in vivo. Moreover, peptidase-inactivated E414D mutant ACE and also PI-PLC rescued the egg-binding deficiency of Ace knockout sperms, implying that ACE plays a crucial role in fertilization through this activity.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Functional rafts in cell membranes
Simons, K., & Ikonen, E. (1997) · Nature · doi:10.1038/42408 · Cited by 9,757
Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus
Li, W., Moore, M. J., Vasilieva, N., Sui, J., Wong, S. K., Berne, M. A., Somasundaran, M., Sullivan, J. L., Luzuriaga, K., Greenough, T. C., et al. (2003) · Nature · doi:10.1038/nature02145 · Cited by 5,923
Angiotensin-converting enzyme 2 is an essential regulator of heart function
Crackower, M. A., Sarao, R., Oudit, G. Y., Yagil, C., Kozieradzki, I., Scanga, S. E., Oliveira-dos-Santos, A. J., da Costa, J., Zhang, L., Pei, Y., et al. (2002) · Nature · doi:10.1038/nature00786 · Cited by 1,733
Cited by
Sperm transport in the female reproductive tract
Suarez, S. S., & Pacey, A. A. (2006) · Human Reproduction Update · doi:10.1093/humupd/dmi047 · Cited by 1,113
The Kallikrein-Kinin System: Current and Future Pharmacological Targets
Moreau, M. E., Garbacki, N., Molinaro, G., Brown, N. J., Marceau, F., & Adam, A. (2005) · Journal of Pharmacological Sciences · doi:10.1254/jphs.srj05001x · Cited by 457
The Concise Guide to PHARMACOLOGY 2013/14: Enzymes
Alexander, S. P. H., Benson, H. E., Faccenda, E., Pawson, A. J., Sharman, J. L., Spedding, M., Peters, J. A., Harmar, A. J., & Collaborators, C. (2013) · British Journal of Pharmacology · doi:10.1111/bph.12451 · Cited by 425
Regulation of sperm storage and movement in the mammalian oviduct
Suarez, S. S. (2008) · The International Journal of Developmental Biology · doi:10.1387/ijdb.072527ss · Cited by 356
Biosynthesis and biology of mammalian GPI-anchored proteins
Kinoshita, T. (2020) · Open Biology · doi:10.1098/rsob.190290 · Cited by 341
Self-citationBernstein, K. E., Ong, F. S., Blackwell, W.-L. B., Shah, K. H., Giani, J. F., Gonzalez-Villalobos, R. A., Shen, X. Z., & Fuchs, S. (2013) · Pharmacological Reviews · doi:10.1124/pr.112.006809 · Cited by 324
Hassan, R., Remaley, A. T., Sampson, M. L., Zhang, J., Cox, D. D., Pingpank, J., Alexander, R., Willingham, M., Pastan, I., & Onda, M. (2006) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-05-1477 · Cited by 296
Biosynthesis, Remodelling and Functions of Mammalian GPI-anchored Proteins: Recent Progress
Kinoshita, T., Fujita, M., & Maeda, Y. (2008) · Journal of Biochemistry · doi:10.1093/jb/mvn090 · Cited by 276
Self-citationMolecular Basis of Human Sperm Capacitation
Puga Molina, L. C., Luque, G. M., Balestrini, P. A., Marín-Briggiler, C. I., Romarowski, A., & Buffone, M. G. (2018) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2018.00072 · Cited by 256
Kinin-dependent hypersensitivity reactions in hemodialysis: metabolic and genetic factors
Molinaro, G., Duan, Q. L., Chagnon, M., Moreau, M. E., Simon, P., Clavel, P., Lavaud, S., Boileau, G., Rouleau, G. A., Lepage, Y., et al. (2006) · Kidney International · doi:10.1038/sj.ki.5001873 · Cited by 29
Metadata
- Authors
- Kondoh, G., Tojo, H., Nakatani, Y., Komazawa, N., Murata, C., Yamagata, K., Maeda, Y., Kinoshita, T., Okabe, M., Taguchi, R., & Takeda, J.
- Venue
- Nature Medicine
- Published
- 2005-01-23
- Volume
- 11
- Issue
- 2
- Pages
- 160-166
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- OpenAlex
- W2146340209
- Cited by
- 208
- References
- 3
- FWCI
- 14.85
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
