The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Mechanics and contraction dynamics of single platelets and implications for clot stiffening
Open accessAbstract
Platelets interact with fibrin polymers to form blood clots at sites of vascular injury1–3. Bulk studies have shown clots to be active materials, with platelet contraction driving the retraction and stiffening of clots4. However, neither the dynamics of single-platelet contraction nor the strength and elasticity of individual platelets, both of which are important for understanding clot material properties, have been directly measured. Here we use atomic force microscopy to measure the mechanics and dynamics of single platelets. We find that platelets contract nearly instantaneously when activated by contact with fibrinogen and complete contraction within 15 min. Individual platelets can generate an average maximum contractile force of 29 nN and form adhesions stronger than 70 nN. Our measurements show that when exposed to stiffer microenvironments, platelets generated higher stall forces, which indicates that platelets may be able to contract heterogeneous clots more uniformly. The high elasticity of individual platelets, measured to be 10 kPa after contraction, combined with their high contractile forces, indicates that clots may be stiffened through direct reinforcement by platelets as well as by strain stiffening of fibrin under tension due to platelet contraction. These results show how the mechanosensitivity and mechanics of single cells can be used to dynamically alter the material properties of physiologic systems.
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Cites
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An active biopolymer network controlled by molecular motors
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Multiscale Mechanics of Fibrin Polymer: Gel Stretching with Protein Unfolding and Loss of Water
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The mechanical properties of fibrin for basic scientists and clinicians
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Human Cartilage Repair with a Photoreactive Adhesive-Hydrogel Composite
Sharma, B., Fermanian, S., Gibson, M., Unterman, S., Herzka, D. A., Cascio, B., Coburn, J., Hui, A. Y., Marcus, N., Gold, G. E., et al. (2013) · Science Translational Medicine · doi:10.1126/scitranslmed.3004838 · Cited by 342
Chen, Z., Lu, J., Zhang, C., Hsia, I., Yu, X., Marecki, L., Marecki, E., Asmani, M., Jain, S., Neelamegham, S., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-10067-6 · Cited by 64
Metadata
- Authors
- Lam, W. A., Chaudhuri, O., Crow, A., Webster, K. D., Li, T.-D., Kita, A., Huang, J., & Fletcher, D. A.
- Venue
- Nature Materials
- Published
- 2010-12-05
- Volume
- 10
- Issue
- 1
- Pages
- 61-66
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W1981248837
- Cited by
- 349
- References
- 25
- FWCI
- 9.10
- Type
- journal article
- Language
- en
- Open access
- green
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- Lam, W. A., Chaudhuri, O., Crow, A., Webster, K. D., Li, T.-D., Kita, A., Huang, J., & Fletcher, D. A. (2011). Mechanics and contraction dynamics of single platelets and implications for clot stiffening. Nature Materials. https://doi.org/10.1038/nmat2903
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
