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Cited by 181

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Simultaneous detection of many T-cell specificities using combinatorial tetramer staining

Open access
Newell, Evan W, Klein, Lawrence O, Yu, Wong, Davis, Mark M·2009·Nature Methods

Abstract

The direct detection of antigen-specific T cells using tetramers of soluble peptide–major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (≥15) T-cell specificities in a single human blood sample.

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Metadata

Authors
Newell, E. W., Klein, L. O., Yu, W., & Davis, M. M.
Venue
Nature Methods
Published
2009-06-21
Volume
6
Issue
7
Pages
497-499
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W1969475094
Cited by
181
References
1
FWCI
5.86
Type
journal article
Language
en
Open access
green

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