The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Simultaneous detection of many T-cell specificities using combinatorial tetramer staining
Open accessAbstract
The direct detection of antigen-specific T cells using tetramers of soluble peptide–major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (≥15) T-cell specificities in a single human blood sample.
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Citations
Cites
Phenotypic Analysis of Antigen-Specific T Lymphocytes
Altman, J. D., Moss, P. A. H., Goulder, P. J. R., Barouch, D. H., McHeyzer-Williams, M. G., Bell, J. I., McMichael, A. J., & Davis, M. M. (1996) · Science · doi:10.1126/science.274.5284.94 · Cited by 3,544
Self-citation
Cited by
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza, A., Chang, H., Radbruch, A., Abrignani, S., Addo, R., Akdis, M., Andrä, I., Andreata, F., Annunziato, F., Arranz, E., et al. (2021) · European Journal of Immunology · doi:10.1002/eji.202170126 · Cited by 531
Huang, H., Wang, C., Rubelt, F., Scriba, T. J., & Davis, M. M. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0505-4 · Cited by 484
Self-citationFlow-cytometric analysis of rare antigen-specific T cells
Bacher, P., & Scheffold, A. (2013) · Cytometry Part A · doi:10.1002/cyto.a.22317 · Cited by 155
Interrogating the repertoire: broadening the scope of peptide–MHC multimer analysis
Davis, M. M., Altman, J. D., & Newell, E. W. (2011) · Nature Reviews Immunology · doi:10.1038/nri3020 · Cited by 124
Self-citationSelf-recognition drives the preferential accumulation of promiscuous CD4(+) T-cells in aged mice
Deshpande, N. R., Parrish, H. L., & Kuhns, M. S. (2015) · eLife · doi:10.7554/elife.05949 · Cited by 35
Metadata
- Authors
- Newell, E. W., Klein, L. O., Yu, W., & Davis, M. M.
- Venue
- Nature Methods
- Published
- 2009-06-21
- Volume
- 6
- Issue
- 7
- Pages
- 497-499
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W1969475094
- Cited by
- 181
- References
- 1
- FWCI
- 5.86
- Type
- journal article
- Language
- en
- Open access
- green
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