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Cited by 432

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis

Open access
Vinkenborg, Jan L, Nicolson, Tamara J, Bellomo, Elisa A, Koay, Melissa S, Rutter, Guy A, Merkx, Maarten·2009·Nature Methods

Abstract

We developed genetically encoded fluorescence resonance energy transfer (FRET)-based sensors that display a large ratiometric change upon Zn2+ binding, have affinities that span the pico- to nanomolar range and can readily be targeted to subcellular organelles. Using this sensor toolbox we found that cytosolic Zn2+ was buffered at 0.4 nM in pancreatic β cells, and we found substantially higher Zn2+ concentrations in insulin-containing secretory vesicles.

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Crossref · 10.1038/nmeth.1368

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Metadata

Authors
Vinkenborg, J. L., Nicolson, T. J., Bellomo, E. A., Koay, M. S., Rutter, G. A., & Merkx, M.
Venue
Nature Methods
Published
2009-08-30
Volume
6
Issue
10
Pages
737-740
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W2110383903
Cited by
432
References
5
FWCI
16.50
Type
journal article
Language
en
Open access
green

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.