Knocking out multigene redundancies via cycles of sexual assortment and fluorescence selection
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Phenotypes that might otherwise reveal a gene's function can be obscured by genes with overlapping function. This phenomenon is best known within gene families, in which an important shared function may only be revealed by mutating all family members. Here we describe the 'green monster' technology that enables precise deletion of many genes. In this method, a population of deletion strains with each deletion marked by an inducible green fluorescent protein reporter gene, is subjected to repeated rounds of mating, meiosis and flow-cytometric enrichment. This results in the aggregation of multiple deletion loci in single cells. The green monster strategy is potentially applicable to assembling other engineered alterations in any species with sex or alternative means of allelic assortment. To test the technology, we generated a single broadly drug-sensitive strain of Saccharomyces cerevisiae bearing precise deletions of all 16 ATP-binding cassette transporters within clades associated with multidrug resistance.
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Only the S. cerevisiae strain lacking YOR1 among 16 ABC transporter deletion strains is sensitive to beauvericin.
“We screened S. cerevisiae strains each lacking one of 16 ATP-binding cassette (ABC) transporter genes14, and found that only the strain lacking YOR1 was sensitive to beauvericin (Supplementary Fig. 2).”
From Dual action antifungal small molecule modulates multidrug efflux and TOR signaling (2016) · cites this paper as [14]
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- Authors
- Suzuki, Y., Onge, R. P. S., Mani, R., King, O. D., Heilbut, A., Labunskyy, V. M., Chen, W., Pham, L., Zhang, L. V., Tong, A. H. Y., Nislow, C., Giaever, G., Gladyshev, V. N., Vidal, M., Schow, P., Lehár, J., & Roth, F. P.
- Affiliations
- Harvard University (US)
- Mount Sinai Hospital (CA)
- Boston University (US)
- Brigham and Women's Hospital (US)
- University of Hong Kong (HK)
- University of Toronto (CA)
- Dana-Farber Cancer Institute (US)
- Corresponding author
- Suzuki, Yo
- ORCID
- Venue
- Nature Methods
- Published
- 2011-01-09
- Volume
- 8
- Issue
- 2
- Pages
- 159-164
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC3076670
- OpenAlex
- W2163052215
- Cited by
- 78
- References
- 34
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 2 · 2023: 7 · 2022: 10 · 2021: 5 · 2020: 2 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 10 · 2015: 7 · 2014: 5 · 2013: 1 · 2012: 6
- FWCI
- 2.33
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Human Genome Research Institute (R01HG003224)
- National Cancer Institute (R21CA130266)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- meiosis, ABC transporters, multidrug resistance, Saccharomyces cerevisiae, drug sensitivity
- MeSH terms
- Multigene Family, Saccharomyces cerevisiae, Gene Deletion, ATP-Binding Cassette Transporters, Green Fluorescent Proteins, Gene Knockout Techniques
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