The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A versatile in vivo system for directed dissection of gene expression patterns
Open accessAbstract
Tissue-specific gene expression using the upstream activating sequence (UAS)–GAL4 binary system has facilitated genetic dissection of many biological processes in Drosophila melanogaster. Refining GAL4 expression patterns or independently manipulating multiple cell populations using additional binary systems are common experimental goals. To simplify these processes, we developed a convertible genetic platform, the integrase swappable in vivo targeting element (InSITE) system. This approach allows GAL4 to be replaced with any other sequence, placing different genetic effectors under the control of the same regulatory elements. Using InSITE, GAL4 can be replaced with LexA or QF, allowing an expression pattern to be repurposed. GAL4 can also be replaced with GAL80 or split-GAL4 hemi-drivers, allowing intersectional approaches to refine expression patterns. The exchanges occur through efficient in vivo manipulations, making it possible to generate many swaps in parallel. This system is modular, allowing future genetic tools to be easily incorporated into the existing framework.
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Wagh, D. A., Rasse, T. M., Asan, E., Hofbauer, A., Schwenkert, I., Dürrbeck, H., Buchner, S., Dabauvalle, M.-C., Schmidt, M., Qin, G., et al. (2006) · Neuron · doi:10.1016/j.neuron.2006.02.008 · Cited by 908
Motor control in a Drosophila taste circuit
Gordon, M. D., & Scott, K. (2009) · Neuron · doi:10.1016/j.neuron.2008.12.033 · Cited by 531
The Neural Substrate of Spectral Preference in Drosophila
Gao, S., Takemura, S.-Y., Ting, C.-Y., Huang, S., Lu, Z., Luan, H., Rister, J., Thum, A. S., Yang, M., Hong, S.-T., et al. (2008) · Neuron · doi:10.1016/j.neuron.2008.08.010 · Cited by 284
Cited by
Venken, K. J. T., Schulze, K. L., Haelterman, N. A., Pan, H., He, Y., Evans-Holm, M., Carlson, J. W., Levis, R. W., Spradling, A. C., Hoskins, R. A., et al. (2011) · Nature Methods · doi:10.1038/nmeth.1662 · Cited by 675
Layered reward signalling through octopamine and dopamine in Drosophila
Burke, C. J., Huetteroth, W., Owald, D., Perisse, E., Krashes, M. J., Das, G., Gohl, D., Silies, M., Certel, S., & Waddell, S. (2012) · Nature · doi:10.1038/nature11614 · Cited by 614
Self-citationGenetic Manipulation of Genes and Cells in the Nervous System of the Fruit Fly
Venken, K. J. T., Simpson, J. H., & Bellen, H. J. (2011) · Neuron · doi:10.1016/j.neuron.2011.09.021 · Cited by 450
Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes
Diao, F., Ironfield, H., Luan, H., Diao, F., Shropshire, W. C., Ewer, J., Marr, E., Potter, C. J., Landgraf, M., & White, B. H. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.01.059 · Cited by 347
Self-citationCellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics
Croset, V., Treiber, C. D., & Waddell, S. (2018) · eLife · doi:10.7554/elife.34550 · Cited by 299
Metadata
- Authors
- Gohl, D. M., Silies, M. A., Gao, X. J., Bhalerao, S., Luongo, F. J., Lin, C.-C., Potter, C. J., & Clandinin, T. R.
- Venue
- Nature Methods
- Published
- 2011-01-30
- Volume
- 8
- Issue
- 3
- Pages
- 231-237
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2037366394
- Cited by
- 219
- References
- 3
- FWCI
- 4.65
- Type
- journal article
- Language
- en
- Open access
- green
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- Gohl, D. M., Silies, M. A., Gao, X. J., Bhalerao, S., Luongo, F. J., Lin, C.-C., Potter, C. J., & Clandinin, T. R. (2011). A versatile in vivo system for directed dissection of gene expression patterns. Nature Methods. https://doi.org/10.1038/nmeth.1561
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