The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A versatile in vivo system for directed dissection of gene expression patterns
Open accessAbstract
Tissue-specific gene expression using the upstream activating sequence (UAS)–GAL4 binary system has facilitated genetic dissection of many biological processes in Drosophila melanogaster. Refining GAL4 expression patterns or independently manipulating multiple cell populations using additional binary systems are common experimental goals. To simplify these processes, we developed a convertible genetic platform, the integrase swappable in vivo targeting element (InSITE) system. This approach allows GAL4 to be replaced with any other sequence, placing different genetic effectors under the control of the same regulatory elements. Using InSITE, GAL4 can be replaced with LexA or QF, allowing an expression pattern to be repurposed. GAL4 can also be replaced with GAL80 or split-GAL4 hemi-drivers, allowing intersectional approaches to refine expression patterns. The exchanges occur through efficient in vivo manipulations, making it possible to generate many swaps in parallel. This system is modular, allowing future genetic tools to be easily incorporated into the existing framework.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Wagh, D. A., Rasse, T. M., Asan, E., Hofbauer, A., Schwenkert, I., Dürrbeck, H., Buchner, S., Dabauvalle, M.-C., Schmidt, M., Qin, G., et al. (2006) · Neuron · doi:10.1016/j.neuron.2006.02.008 · Cited by 908
Motor control in a Drosophila taste circuit
Gordon, M. D., & Scott, K. (2009) · Neuron · doi:10.1016/j.neuron.2008.12.033 · Cited by 531
The Neural Substrate of Spectral Preference in Drosophila
Gao, S., Takemura, S.-Y., Ting, C.-Y., Huang, S., Lu, Z., Luan, H., Rister, J., Thum, A. S., Yang, M., Hong, S.-T., et al. (2008) · Neuron · doi:10.1016/j.neuron.2008.08.010 · Cited by 284
Cited by
Venken, K. J. T., Schulze, K. L., Haelterman, N. A., Pan, H., He, Y., Evans-Holm, M., Carlson, J. W., Levis, R. W., Spradling, A. C., Hoskins, R. A., et al. (2011) · Nature Methods · doi:10.1038/nmeth.1662 · Cited by 675
Layered reward signalling through octopamine and dopamine in Drosophila
Burke, C. J., Huetteroth, W., Owald, D., Perisse, E., Krashes, M. J., Das, G., Gohl, D., Silies, M., Certel, S., & Waddell, S. (2012) · Nature · doi:10.1038/nature11614 · Cited by 614
Self-citationGenetic Manipulation of Genes and Cells in the Nervous System of the Fruit Fly
Venken, K. J. T., Simpson, J. H., & Bellen, H. J. (2011) · Neuron · doi:10.1016/j.neuron.2011.09.021 · Cited by 450
Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes
Diao, F., Ironfield, H., Luan, H., Diao, F., Shropshire, W. C., Ewer, J., Marr, E., Potter, C. J., Landgraf, M., & White, B. H. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.01.059 · Cited by 347
Self-citationCellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics
Croset, V., Treiber, C. D., & Waddell, S. (2018) · eLife · doi:10.7554/elife.34550 · Cited by 299
Genetic Reagents for Making Split-GAL4 Lines in Drosophila
Dionne, H., Hibbard, K. L., Cavallaro, A., Kao, J.-C., & Rubin, G. M. (2018) · Genetics · doi:10.1534/genetics.118.300682 · Cited by 232
Cellular evidence for efference copy in Drosophila visuomotor processing
Kim, A. J., Fitzgerald, J. K., & Maimon, G. (2015) · Nature Neuroscience · doi:10.1038/nn.4083 · Cited by 187
Coupled sensing of hunger and thirst signals balances sugar and water consumption
Jourjine, N., Mullaney, B. C., Mann, K., & Scott, K. (2016) · Cell · doi:10.1016/j.cell.2016.06.046 · Cited by 154
Kondo, S., Takahashi, T., Yamagata, N., Imanishi, Y., Katow, H., Hiramatsu, S., Lynn, K., Abe, A., Kumaraswamy, A., & Tanimoto, H. (2020) · Cell Reports · doi:10.1016/j.celrep.2019.12.018 · Cited by 154
A Membrane Transporter Is Required for Steroid Hormone Uptake in Drosophila
Okamoto, N., Viswanatha, R., Bittar, R., Li, Z., Haga-Yamanaka, S., Perrimon, N., & Yamanaka, N. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.09.012 · Cited by 144
The manifold structure of limb coordination in walking Drosophila
DeAngelis, B. D., Zavatone-Veth, J. A., & Clark, D. A. (2019) · eLife · doi:10.7554/elife.46409 · Cited by 104
Dolan, M.-J., Belliart-Guérin, G., Bates, A. S., Frechter, S., Lampin-Saint-Amaux, A., Aso, Y., Roberts, R. J. V., Schlegel, P., Wong, A., Hammad, A., et al. (2018) · Neuron · doi:10.1016/j.neuron.2018.08.037 · Cited by 118
SPARC enables genetic manipulation of precise proportions of cells
Isaacman-Beck, J., Paik, K. C., Wienecke, C. F. R., Yang, H. H., Fisher, Y. E., Wang, I. E., Ishida, I. G., Maimon, G., Wilson, R. I., & Clandinin, T. R. (2020) · Nature Neuroscience · doi:10.1038/s41593-020-0668-9 · Cited by 77
Self-citationThe Drosophila Split Gal4 System for Neural Circuit Mapping
Luan, H., Diao, F., Scott, R. L., & White, B. H. (2020) · Frontiers in Neural Circuits · doi:10.3389/fncir.2020.603397 · Cited by 73
A Pair of Interneurons Influences the Choice between Feeding and Locomotion in Drosophila
Mann, K., Gordon, M. D., & Scott, K. (2013) · Neuron · doi:10.1016/j.neuron.2013.06.018 · Cited by 66
Visual processing in the fly, from photoreceptors to behavior
Currier, T. A., Pang, M. M., & Clandinin, T. R. (2023) · GENETICS · doi:10.1093/genetics/iyad064 · Cited by 63
Self-citationGliotransmission of D-serine promotes thirst-directed behaviors in Drosophila
Park, A., Croset, V., Otto, N., Agarwal, D., Treiber, C. D., Meschi, E., Sims, D., & Waddell, S. (2022) · Current Biology · doi:10.1016/j.cub.2022.07.038 · Cited by 45
Object-Displacement-Sensitive Visual Neurons Drive Freezing in Drosophila
Tanaka, R., & Clark, D. A. (2020) · Current Biology · doi:10.1016/j.cub.2020.04.068 · Cited by 44
Specific kinematics and motor-related neurons for aversive chemotaxis in Drosophila
Gao, X. J., Potter, C. J., Gohl, D. M., Silies, M., Katsov, A. Y., Clandinin, T. R., & Luo, L. (2013) · Current Biology · doi:10.1016/j.cub.2013.05.008 · Cited by 32
Metadata
- Authors
- Gohl, D. M., Silies, M. A., Gao, X. J., Bhalerao, S., Luongo, F. J., Lin, C.-C., Potter, C. J., & Clandinin, T. R.
- Venue
- Nature Methods
- Published
- 2011-01-30
- Volume
- 8
- Issue
- 3
- Pages
- 231-237
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2037366394
- Cited by
- 219
- References
- 3
- FWCI
- 4.65
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
