The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Fast, three-dimensional super-resolution imaging of live cells
Open accessAbstract
We report super-resolution fluorescence imaging of live cells with high spatiotemporal resolution using stochastic optical reconstruction microscopy (STORM). By labeling proteins either directly or via SNAP tags with photoswitchable dyes, we obtained two-dimensional (2D) and 3D super-resolution images of living cells, using clathrin-coated pits and the transferrin cargo as model systems. Bright, fast-switching probes enabled us to achieve 2D imaging at spatial resolutions of ∼25 nm and temporal resolutions as fast as 0.5 s. We also demonstrated live-cell 3D super-resolution imaging. We obtained 3D spatial resolution of ∼30 nm in the lateral direction and ∼50 nm in the axial direction at time resolutions as fast as 1–2 s with several independent snapshots. Using photoswitchable dyes with distinct emission wavelengths, we also demonstrated two-color 3D super-resolution imaging in live cells. These imaging capabilities open a new window for characterizing cellular structures in living cells at the ultrastructural level.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Cited by
Molecular mechanism and physiological functions of clathrin-mediated endocytosis
McMahon, H. T., & Boucrot, E. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3151 · Cited by 2,223
Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging
Dempsey, G. T., Vaughan, J. C., Chen, K. H., Bates, M., & Zhuang, X. (2011) · Nature Methods · doi:10.1038/nmeth.1768 · Cited by 1,369
Self-citationFluorescence nanoscopy in cell biology
Sahl, S. J., Hell, S. W., & Jakobs, S. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.71 · Cited by 990
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
Lukinavičius, G., Umezawa, K., Olivier, N., Honigmann, A., Yang, G., Plass, T., Mueller, V., Reymond, L., Corrêa Jr, I. R., Luo, Z.-G., et al. (2013) · Nature Chemistry · doi:10.1038/nchem.1546 · Cited by 893
Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 747
Visualizing and discovering cellular structures with super-resolution microscopy
Sigal, Y. M., Zhou, R., & Zhuang, X. (2018) · Science · doi:10.1126/science.aau1044 · Cited by 718
Self-citationLow-dose metformin targets the lysosomal AMPK pathway through PEN2
Ma, T., Tian, X., Zhang, B., Li, M., Wang, Y., Yang, C., Wu, J., Wei, X., Qu, Q., Yu, Y., et al. (2022) · Nature · doi:10.1038/s41586-022-04431-8 · Cited by 644
Light-induced cell damage in live-cell super-resolution microscopy
Wäldchen, S., Lehmann, J., Klein, T., van de Linde, S., & Sauer, M. (2015) · Scientific Reports · doi:10.1038/srep15348 · Cited by 536
Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms
Huang, F., Hartwich, T. M. P., Rivera-Molina, F. E., Lin, Y., Duim, W. C., Long, J. J., Uchil, P. D., Myers, J. R., Baird, M. A., Mothes, W., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2488 · Cited by 526
Shim, S.-H., Xia, C., Zhong, G., Babcock, H. P., Vaughan, J. C., Huang, B., Wang, X., Xu, C., Bi, G.-Q., & Zhuang, X. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1201882109 · Cited by 514
Self-citationPrecisely and accurately localizing single emitters in fluorescence microscopy
Deschout, H., Zanacchi, F. C., Mlodzianoski, M., Diaspro, A., Bewersdorf, J., Hess, S. T., & Braeckmans, K. (2014) · Nature Methods · doi:10.1038/nmeth.2843 · Cited by 498
Uno, S.-N., Kamiya, M., Yoshihara, T., Sugawara, K., Okabe, K., Tarhan, M. C., Fujita, H., Funatsu, T., Okada, Y., Tobita, S., et al. (2014) · Nature Chemistry · doi:10.1038/nchem.2002 · Cited by 469
A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy
Wang, L., Tran, M., D’Este, E., Roberti, J., Koch, B., Xue, L., & Johnsson, K. (2020) · Nature Chemistry · doi:10.1038/s41557-019-0371-1 · Cited by 382
Wang, S., Moffitt, J. R., Dempsey, G. T., Xie, X. S., & Zhuang, X. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1406593111 · Cited by 337
Self-citationLiu, X., Qiao, Q., Tian, W., Liu, W., Chen, J., Lang, M. J., & Xu, Z. (2016) · Journal of the American Chemical Society · doi:10.1021/jacs.6b03924 · Cited by 334
Tracking single molecules at work in living cells
Kusumi, A., Tsunoyama, T. A., Hirosawa, K. M., Kasai, R. S., & Fujiwara, T. K. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1558 · Cited by 332
Switchable Fluorophores for Single-Molecule Localization Microscopy
Li, H., & Vaughan, J. C. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00767 · Cited by 319
Regulation of beta-amyloid production in neurons by astrocyte-derived cholesterol
Wang, H., Kulas, J. A., Wang, C., Holtzman, D. M., Ferris, H. A., & Hansen, S. B. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2102191118 · Cited by 301
Submicrometre geometrically encoded fluorescent barcodes self-assembled from DNA
Lin, C., Jungmann, R., Leifer, A. M., Li, C., Levner, D., Church, G. M., Shih, W. M., & Yin, P. (2012) · Nature Chemistry · doi:10.1038/nchem.1451 · Cited by 302
Imaging proteins inside cells with fluorescent tags
Crivat, G., & Taraska, J. W. (2012) · Trends in Biotechnology · doi:10.1016/j.tibtech.2011.08.002 · Cited by 291
Veatch, S. L., Machta, B. B., Shelby, S. A., Chiang, E. N., Holowka, D. A., & Baird, B. A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0031457 · Cited by 290
Raman spectroscopy: an evolving technique for live cell studies
Smith, R., Wright, K. L., & Ashton, L. (2016) · The Analyst · doi:10.1039/c6an00152a · Cited by 285
Diels–Alder Cycloaddition for Fluorophore Targeting to Specific Proteins inside Living Cells
Liu, D. S., Tangpeerachaikul, A., Selvaraj, R., Taylor, M. T., Fox, J. M., & Ting, A. Y. (2012) · Journal of the American Chemical Society · doi:10.1021/ja209325n · Cited by 254
Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique
Valli, J., Garcia-Burgos, A., Rooney, L. M., Vale de Melo e Oliveira, B., Duncan, R. R., & Rickman, C. (2021) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2021.100791 · Cited by 159
Nanoscopy of living brain slices with low light levels
Testa, I., Urban, N. T., Jakobs, S., Eggeling, C., Willig, K. I., & Hell, S. W. (2012) · Neuron · doi:10.1016/j.neuron.2012.07.028 · Cited by 131
Petersen, E. N., Pavel, M. A., Wang, H., & Hansen, S. B. (2020) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2019.183091 · Cited by 36
Optimization of cell morphology measurement via single-molecule tracking PALM
Frost, N. A., Lu, H. E., & Blanpied, T. A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0036751 · Cited by 22
Metadata
- Authors
- Jones, S. A., Shim, S.-H., He, J., & Zhuang, X.
- Venue
- Nature Methods
- Published
- 2011-05-08
- Volume
- 8
- Issue
- 6
- Pages
- 499-505
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2007971802
- Cited by
- 752
- References
- 2
- FWCI
- 48.42
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
