The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
High-content behavioral analysis of Caenorhabditis elegans in precise spatiotemporal chemical environments
Open accessAbstract
To quantitatively understand chemosensory behaviors, it is desirable to present many animals with repeatable, well-defined chemical stimuli. To that end, we describe a microfluidic system to analyze Caenorhabditis elegans behavior in defined temporal and spatial stimulus patterns. A 2 cm × 2 cm structured arena allowed C. elegans to perform crawling locomotion in a controlled liquid environment. We characterized behavioral responses to attractive odors with three stimulus patterns: temporal pulses, spatial stripes and a linear concentration gradient, all delivered in the fluid phase to eliminate variability associated with air-fluid transitions. Different stimulus configurations preferentially revealed turning dynamics in a biased random walk, directed orientation into an odor stripe and speed regulation by odor. We identified both expected and unexpected responses in wild-type worms and sensory mutants by quantifying dozens of behavioral parameters. The devices are inexpensive, easy to fabricate, reusable and suitable for delivering any liquid-borne stimulus.
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Cites
Parallel Use of Two Behavioral Mechanisms for Chemotaxis in Caenorhabditis elegans
Iino, Y., & Yoshida, K. (2009) · The Journal of Neuroscience · doi:10.1523/jneurosci.3633-08.2009 · Cited by 264
Neuropeptide feedback modifies odor-evoked dynamics in Caenorhabditis elegans olfactory neurons
Chalasani, S. H., Kato, S., Albrecht, D. R., Nakagawa, T., Abbott, L. F., & Bargmann, C. I. (2010) · Nature Neuroscience · doi:10.1038/nn.2526 · Cited by 237
Enhanced Caenorhabditis elegans Locomotion in a Structured Microfluidic Environment
Park, S., Hwang, H., Nam, S.-W., Martinez, F., Austin, R. H., & Ryu, W. S. (2008) · PLoS ONE · doi:10.1371/journal.pone.0002550 · Cited by 157 · cited 1×
“…for neuronal imaging, high-throughput phenotypic screens, and laser axotomy (reviewed in 11,12 ), C. elegans could be completely immobilized, spatially restricted while permitting body posture movements 13 , or allowed to navigate through undulating channels 14 or structured environments 14,15 .”
Cited by
Micro Total Analysis Systems for Cell Biology and Biochemical Assays
Kovarik, M. L., Gach, P. C., Ornoff, D. M., Wang, Y., Balowski, J., Farrag, L., & Allbritton, N. L. (2012) · Analytical Chemistry · doi:10.1021/ac202611x · Cited by 270
How Caenorhabditis elegans Senses Mechanical Stress, Temperature, and Other Physical Stimuli
Goodman, M. B., & Sengupta, P. (2019) · Genetics · doi:10.1534/genetics.118.300241 · Cited by 131
Genetic contributions to behavioural diversity at the gene-environment interface
Bendesky, A., & Bargmann, C. I. (2011) · Nature Reviews Genetics · doi:10.1038/nrg3065 · Cited by 103
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Metadata
- Authors
- Albrecht, D. R., & Bargmann, C. I.
- Venue
- Nature Methods
- Published
- 2011-06-12
- Volume
- 8
- Issue
- 7
- Pages
- 599-605
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2141911005
- Cited by
- 244
- References
- 3
- FWCI
- 6.19
- Type
- journal article
- Language
- en
- Open access
- green
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