The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes
Open accessAbstract
We demonstrate the versatility of a collection of insertions of the transposon Minos-mediated integration cassette (MiMIC), in Drosophila melanogaster. MiMIC contains a gene-trap cassette and the yellow+ marker flanked by two inverted bacteriophage ΦC31 integrase attP sites. MiMIC integrates almost at random in the genome to create sites for DNA manipulation. The attP sites allow the replacement of the intervening sequence of the transposon with any other sequence through recombinase-mediated cassette exchange (RMCE). We can revert insertions that function as gene traps and cause mutant phenotypes to revert to wild type by RMCE and modify insertions to control GAL4 or QF overexpression systems or perform lineage analysis using the Flp recombinase system. Insertions in coding introns can be exchanged with protein-tag cassettes to create fusion proteins to follow protein expression and perform biochemical experiments. The applications of MiMIC vastly extend the D. melanogaster toolkit.
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Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
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Liu, L., MacKenzie, K. R., Putluri, N., Maletić-Savatić, M., & Bellen, H. J. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.08.024 · Cited by 556
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Rui, Y.-N., Xu, Z., Patel, B., Chen, Z., Chen, D., Tito, A., David, G., Sun, Y., Stimming, E. F., Bellen, H. J., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3101 · Cited by 423
Self-citationTranscriptome-wide distribution and function of RNA hydroxymethylcytosine
Delatte, B., Wang, F., Ngoc, L. V., Collignon, E., Bonvin, E., Deplus, R., Calonne, E., Hassabi, B., Putmans, P., Awe, S., et al. (2016) · Science · doi:10.1126/science.aac5253 · Cited by 411
A genome-wide resource for the analysis of protein localisation in Drosophila
Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., Stender, B., Janosch, S., KJ, V. V., Krishnan, R., et al. (2016) · eLife · doi:10.7554/elife.12068 · Cited by 359
Chemoconnectomics: Mapping Chemical Transmission in Drosophila
Deng, B., Li, Q., Liu, X., Cao, Y., Li, B., Qian, Y., Xu, R., Mao, R., Zhou, E., Zhang, W., et al. (2019) · Neuron · doi:10.1016/j.neuron.2019.01.045 · Cited by 340
Metadata
- Authors
- Venken, K. J. T., Schulze, K. L., Haelterman, N. A., Pan, H., He, Y., Evans-Holm, M., Carlson, J. W., Levis, R. W., Spradling, A. C., Hoskins, R. A., & Bellen, H. J.
- Venue
- Nature Methods
- Published
- 2011-07-31
- Volume
- 8
- Issue
- 9
- Pages
- 737-743
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2122132730
- Cited by
- 675
- References
- 1
- FWCI
- 8.35
- Type
- journal article
- Language
- en
- Open access
- green
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