The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Precise and reliable gene expression via standard transcription and translation initiation elements
Open accessAbstract
An inability to reliably predict quantitative behaviors for novel combinations of genetic elements limits the rational engineering of biological systems. We developed an expression cassette architecture for genetic elements controlling transcription and translation initiation in Escherichia coli: transcription elements encode a common mRNA start, and translation elements use an overlapping genetic motif found in many natural systems. We engineered libraries of constitutive and repressor-regulated promoters along with translation initiation elements following these definitions. We measured activity distributions for each library and selected elements that collectively resulted in expression across a 1,000-fold observed dynamic range. We studied all combinations of curated elements, demonstrating that arbitrary genes are reliably expressed to within twofold relative target expression windows with ∼93% reliability. We expect the genetic element definitions validated here can be collectively expanded to create collections of public-domain standard biological parts that support reliable forward engineering of gene expression at genome scales.
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Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
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Foundations for engineering biology
Endy, D. (2005) · Nature · doi:10.1038/nature04342 · Cited by 1,511
Self-citationCoding-Sequence Determinants of Gene Expression in Escherichia coli
Kudla, G., Murray, A. W., Tollervey, D., & Plotkin, J. B. (2009) · Science · doi:10.1126/science.1170160 · Cited by 1,458
The second wave of synthetic biology: from modules to systems
Purnick, P. E. M., & Weiss, R. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2698 · Cited by 1,181
Five hard truths for synthetic biology
Kwok, R. (2010) · Nature · doi:10.1038/463288a · Cited by 442
Next-generation synthetic gene networks
Lu, T. K., Khalil, A. S., & Collins, J. J. (2009) · Nature Biotechnology · doi:10.1038/nbt.1591 · Cited by 365
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Nielsen, A. A. K., Der, B. S., Shin, J., Vaidyanathan, P., Paralanov, V., Strychalski, E. A., Ross, D., Densmore, D., & Voigt, C. A. (2016) · Science · doi:10.1126/science.aac7341 · Cited by 1,020
A brief history of synthetic biology
Cameron, D. E., Bashor, C. J., & Collins, J. J. (2014) · Nature Reviews Microbiology · doi:10.1038/nrmicro3239 · Cited by 933
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Self-citationGrowing a circular economy with fungal biotechnology: a white paper
Meyer, V., Basenko, E. Y., Benz, J. P., Braus, G. H., Caddick, M. X., Csukai, M., de Vries, R. P., Endy, D., Frisvad, J. C., Gunde-Cimerman, N., et al. (2020) · Fungal Biology and Biotechnology · doi:10.1186/s40694-020-00095-z · Cited by 515
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Metadata
- Authors
- Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q.-A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P., & Endy, D.
- Venue
- Nature Methods
- Published
- 2013-03-10
- Volume
- 10
- Issue
- 4
- Pages
- 354-360
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2001426985
- Cited by
- 764
- References
- 8
- FWCI
- 28.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
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