Large-scale de novo DNA synthesis: technologies and applications

Enzymatic assembly of DNA molecules up to several hundred kilobases

Gibson, D. G., Young, L., Chuang, R.-Y., Venter, J. C., Hutchison, C. A., & Smith, H. O. (2009) · Nature Methods · doi:10.1038/nmeth.1318 · Cited by 9,742 · see citing works

Folding DNA to create nanoscale shapes and patterns

Rothemund, P. W. K. (2006) · Nature · doi:10.1038/nature04586 · Cited by 7,391 · see citing works

Genome-scale CRISPR-Cas9 knockout screening in human cells

Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., et al. (2014) · Science · doi:10.1126/science.1247005 · Cited by 5,234 · see citing works

Genetic screens in human cells using the CRISPR-Cas9 system

Wang, T., Wei, J. J., Sabatini, D. M., & Lander, E. S. (2014) · Science · doi:10.1126/science.1246981 · Cited by 2,856 · see citing works

Light-Directed, Spatially Addressable Parallel Chemical Synthesis

Fodor, S. P. A., Read, J., Pirrung, M. C., Stryer, L., Lu, A. T., & Solas, D. W. (1991) · Science · doi:10.1126/science.1990438 · Cited by 2,697 · see citing works

Self-assembly of DNA into nanoscale three-dimensional shapes

Douglas, S. M., Dietz, H., Liedl, T., Högberg, B., Graf, F., & Shih, W. M. (2009) · Nature · doi:10.1038/nature08016 · Cited by 2,579 · see citing works

A Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads

Douglas, S. M., Bachelet, I., & Church, G. M. (2012) · Science · doi:10.1126/science.1214081 · Cited by 2,187 · see citing works

Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis

Beaucage, S. L., & Caruthers, M. H. (1981) · Tetrahedron Letters · doi:10.1016/s0040-4039(01)90461-7 · Cited by 2,013 · see citing works

The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides

Nirenberg, M. W., & Matthaei, J. (1961) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.47.10.1588 · Cited by 1,638 · see citing works

Coding-Sequence Determinants of Gene Expression in <i>Escherichia coli</i>

Kudla, G., Murray, A. W., Tollervey, D., & Plotkin, J. B. (2009) · Science · doi:10.1126/science.1170160 · Cited by 1,458 · see citing works

Next-Generation Digital Information Storage in DNA

Church, G. M., Gao, Y., & Kosuri, S. (2012) · Science · doi:10.1126/science.1226355 · Cited by 1,441 · see citing works

Scaling Up Digital Circuit Computation with DNA Strand Displacement Cascades

Qian, L., & Winfree, E. (2011) · Science · doi:10.1126/science.1200520 · Cited by 1,440 · see citing works

Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing

Gnirke, A., Melnikov, A., Maguire, J., Rogov, P., LeProust, E. M., Brockman, W., Fennell, T., Giannoukos, G., Fisher, S., Russ, C., et al. (2009) · Nature Biotechnology · doi:10.1038/nbt.1523 · Cited by 1,433 · cited 1× · see citing works

A Modular Cloning System for Standardized Assembly of Multigene Constructs

Weber, E., Engler, C., Gruetzner, R., Werner, S., & Marillonnet, S. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016765 · Cited by 1,385 · see citing works

Light-generated oligonucleotide arrays for rapid DNA sequence analysis.

Pease, A. C., Solas, D. W., Sullivan, E. J., Cronin, M., Holmes, C. P., & Fodor, S. P. A. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.11.5022 · Cited by 1,366 · see citing works

Folding DNA into Twisted and Curved Nanoscale Shapes

Dietz, H., Douglas, S. M., & Shih, W. M. (2009) · Science · doi:10.1126/science.1174251 · Cited by 1,340 · see citing works

Characterization of the reconstructed 1918 Spanish influenza pandemic virus

Tumpey, T. M., Basler, C. F., Aguilar, P. V., Zeng, H., Solórzano, A., Swayne, D. E., Cox, N. J., Katz, J. M., Taubenberger, J. K., Palese, P., et al. (2005) · Science · doi:10.1126/science.1119392 · Cited by 1,327 · see citing works

Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer

Hughes, T. R., Mao, M., Jones, A. R., Burchard, J., Marton, M. J., Shannon, K. W., Lefkowitz, S. M., Ziman, M., Schelter, J. M., Meyer, M. R., et al. (2001) · Nature Biotechnology · doi:10.1038/86730 · Cited by 1,298 · see citing works

The DNA-encoded nucleosome organization of a eukaryotic genome

Kaplan, N., Moore, I. K., Fondufe-Mittendorf, Y., Gossett, A., Tillo, D., Field, Y., Leproust, E., Hughes, T., Lieb, J., Widom, J., et al. (2008) · Nature · doi:10.1038/nature07667 · Cited by 1,296 · see citing works

Three-Dimensional Structures Self-Assembled from DNA Bricks

Ke, Y., Ong, L. L., Shih, W. M., & Yin, P. (2012) · Science · doi:10.1126/science.1227268 · Cited by 1,249 · see citing works

Towards Practical, High-Capacity, Low-Maintenance Information Storage in Synthesized DNA

Goldman, N., Bertone, P., Chen, S., Dessimoz, C., LeProust, E. M., Sipos, B., & Birney, E. (2013) · Nature · doi:10.1038/nature11875 · Cited by 1,220 · see citing works

Neural network computation with DNA strand displacement cascades

Qian, L., Winfree, E., & Bruck, J. (2011) · Nature · doi:10.1038/nature10262 · Cited by 1,079 · see citing works

Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template.

Cello, J., Paul, A. V., & Wimmer, E. (2002) · Science · doi:10.1126/science.1072266 · Cited by 980 · see citing works

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC

Li, M. Z., & Elledge, S. J. (2007) · Nature Methods · doi:10.1038/nmeth1010 · Cited by 939 · see citing works

Principles of genetic circuit design

Brophy, J. A. N., & Voigt, C. A. (2014) · Nature Methods · doi:10.1038/nmeth.2926 · Cited by 934 · see citing works

Precise and reliable gene expression via standard transcription and translation initiation elements

Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C. S. C., Christoffersen, M. J., Mai, Q.-A., Tran, A. B., Paull, M. L., Keasling, J. D., Arkin, A. P., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2404 · Cited by 748 · see citing works

Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array

Singh‐Gasson, S., Green, R. D., Yue, Y., Nelson, C. J., Blattner, F. R., Sussman, M. R., & Cerrina, F. (1999) · Nature Biotechnology · doi:10.1038/13664 · Cited by 733 · see citing works

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

Stemmer, W. P. C., Crameri, A., Ha, K. D., Brennan, T. M., & Heyneker, H. L. (1995) · Gene · doi:10.1016/0378-1119(95)00511-4 · Cited by 732 · see citing works

Virus attenuation by genome-scale changes in codon pair bias

Coleman, J. R., Papamichail, D., Skiena, S., Futcher, B., Wimmer, E., & Mueller, S. (2008) · Science · doi:10.1126/science.1155761 · Cited by 715 · see citing works

Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay

Melnikov, A., Murugan, A., Zhang, X., Tesileanu, T., Wang, L., Rogov, P., Feizi, S., Gnirke, A., Callan, C. G., Kinney, J. B., et al. (2012) · Nature Biotechnology · doi:10.1038/nbt.2137 · Cited by 711 · see citing works

DNA as a universal substrate for chemical kinetics

Soloveichik, D., Seelig, G., & Winfree, E. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0909380107 · Cited by 695 · see citing works

Self-assembly of carbon nanotubes into two-dimensional geometries using DNA origami templates

Maune, H., Han, S., Barish, R. D., Bockrath, M., Goddard, W. A., Rothemund, P. W. K., & Winfree, E. (2009) · Nature Nanotechnology · doi:10.1038/nnano.2009.311 · Cited by 622 · see citing works

Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides

Smith, H. O., Hutchison, C. A., Pfannkoch, C. M., & Venter, J. C. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2237126100 · Cited by 612 · see citing works

Causes and effects of N-terminal codon bias in bacterial genes

Goodman, D. B., Church, G. M., & Kosuri, S. (2013) · Science · doi:10.1126/science.1241934 · Cited by 573 · see citing works

Genome-Wide Identification of Human RNA Editing Sites by Parallel DNA Capturing and Sequencing

Li, J. B., Levanon, E. Y., Yoon, J.-K., Aach, J., Xie, B., LeProust, E. M., Zhang, K., Gao, Y., & Church, G. M. (2009) · Science · doi:10.1126/science.1170995 · Cited by 525 · see citing works

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

Quan, J., & Tian, J. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006441 · Cited by 519 · see citing works

High-resolution mapping of protein sequence-function relationships

Fowler, D. M., Araya, C. L., Fleishman, S. J., Kellogg, E. H., Stephany, J. J., Baker, D., & Fields, S. (2010) · Nature Methods · doi:10.1038/nmeth.1492 · Cited by 516 · see citing works

SLiCE: a novel bacterial cell extract-based DNA cloning method

Zhang, Y., Werling, U., & Edelmann, W. (2012) · Nucleic Acids Research · doi:10.1093/nar/gkr1288 · Cited by 498 · see citing works

Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming

Deng, J., Shoemaker, R., Xie, B., Gore, A., LeProust, E. M., Antosiewicz‐Bourget, J., Egli, D. M., Maherali, N., Park, I., Yu, J., et al. (2009) · Nature Biotechnology · doi:10.1038/nbt.1530 · Cited by 491 · see citing works

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

Gibson, D. G., Benders, G. A., Axelrod, K. C., Zaveri, J., Algire, M. A., Moodie, M., Montague, M. G., Venter, J. C., Smith, H. O., & Hutchison, C. A. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0811011106 · Cited by 480 · see citing works

Characterization of 582 natural and synthetic terminators and quantification of their design constraints

Chen, Y.-J., Liu, P., Nielsen, A. A. K., Brophy, J. A. N., Clancy, K., Peterson, T., & Voigt, C. A. (2013) · Nature Methods · doi:10.1038/nmeth.2515 · Cited by 477 · see citing works

Synthetic chromosome arms function in yeast and generate phenotypic diversity by design

Dymond, J. S., Richardson, S. M., Coombes, C. E., Babatz, T. D., Muller, H., Annaluru, N., Blake, W. J., Schwerzmann, J., Dai, J., Lindstrom, D. L., et al. (2011) · Nature · doi:10.1038/nature10403 · Cited by 476 · see citing works

The spatial architecture of protein function and adaptation

McLaughlin, R. N., Poelwijk, F. J., Raman, A., Gosal, W. S., & Ranganathan, R. (2012) · Nature · doi:10.1038/nature11500 · Cited by 462 · see citing works

Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters

Sharon, E., Kalma, Y., Sharp, A., Raveh-Sadka, T., Levo, M., Zeevi, D., Keren, L., Yakhini, Z., Weinberger, A., & Segal, E. (2012) · Nature Biotechnology · doi:10.1038/nbt.2205 · Cited by 457 · see citing works

Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca

Temme, K., Zhao, D., & Voigt, C. A. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1120788109 · Cited by 421 · see citing works

Multiplex amplification of large sets of human exons

Porreca, G. J., Zhang, K., Li, J. B., Xie, B., Austin, D., Vassallo, S., LeProust, E. M., Peck, B. J., Emig, C. J., Dahl, F., et al. (2007) · Nature Methods · doi:10.1038/nmeth1110 · Cited by 410 · see citing works

Accurate multiplex gene synthesis from programmable DNA microchips

Tian, J., GONG, H. R., Sheng, N., Zhou, X., Gülari, E., Gao, X., & Church, G. M. (2004) · Nature · doi:10.1038/nature03151 · Cited by 408 · see citing works

Composability of regulatory sequences controlling transcription and translation in <i>Escherichia coli</i>

Kosuri, S., Goodman, D. B., Cambray, G., Mutalik, V. K., Gao, Y., Arkin, A. P., Endy, D., & Church, G. M. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1301301110 · Cited by 392 · see citing works

Reduction of the Rate of Poliovirus Protein Synthesis through Large-Scale Codon Deoptimization Causes Attenuation of Viral Virulence by Lowering Specific Infectivity

Mueller, S., Papamichail, D., Coleman, J. R., Skiena, S., & Wimmer, E. (2006) · Journal of Virology · doi:10.1128/jvi.00738-06 · Cited by 383 · see citing works

High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis

Patwardhan, R. P., Lee, C., Litvin, O., Young, D. L., Pe'er, D., & Shendure, J. (2009) · Nature Biotechnology · doi:10.1038/nbt.1589 · Cited by 377 · see citing works

Genomic mining of prokaryotic repressors for orthogonal logic gates

Stanton, B. C., Nielsen, A. A. K., Tamsir, A., Clancy, K., Peterson, T., & Voigt, C. A. (2013) · Nature Chemical Biology · doi:10.1038/nchembio.1411 · Cited by 369 · see citing works

Refactoring bacteriophage T7

Chan, L. Y., Kosuri, S., & Endy, D. (2005) · Molecular Systems Biology · doi:10.1038/msb4100025 · Cited by 359 · see citing works

Autoantigen discovery with a synthetic human peptidome

Larman, H. B., Zhao, Z., Laserson, U., Li, M. Z., Ciccia, A., Gakidis, M. A. M., Church, G. M., Kesari, S., LeProust, E. M., Solimini, N. L., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1856 · Cited by 354 · see citing works

Hot Spots for Allosteric Regulation on Protein Surfaces

Reynolds, K. A., McLaughlin, R. N., & Ranganathan, R. (2011) · Cell · doi:10.1016/j.cell.2011.10.049 · Cited by 350 · see citing works

Chemical synthesis of the mouse mitochondrial genome

Gibson, D. G., Smith, H. O., Hutchison, C. A., Venter, J. C., & Merryman, C. (2010) · Nature Methods · doi:10.1038/nmeth.1515 · Cited by 348 · see citing works

An autonomous polymerization motor powered by DNA hybridization

Venkataraman, S., Dirks, R. M., Rothemund, P. W. K., Winfree, E., & Pierce, N. A. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.225 · Cited by 340 · see citing works

High-density oligonucleotide arrays

Blanchard, A. P., Kaiser, R. J., & Hood, L. (1996) · Biosensors and Bioelectronics · doi:10.1016/0956-5663(96)83302-1 · Cited by 334 · see citing works

Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process

LeProust, E. M., Peck, B. J., Spirin, K., McCuen, H. B., Moore, B., Namsaraev, E., & Caruthers, M. H. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq163 · Cited by 326 · see citing works

Systematic dissection of regulatory motifs in 2000 predicted human enhancers using a massively parallel reporter assay

Kheradpour, P., Ernst, J., Melnikov, A., Rogov, P., Wang, L., Zhang, X., Alston, J., Mikkelsen, T. S., & Kellis, M. (2013) · Genome Research · doi:10.1101/gr.144899.112 · Cited by 315 · see citing works

Reconstitution of an Infectious Human Endogenous Retrovirus

Lee, Y. N., & Bieniasz, P. D. (2007) · PLoS Pathogens · doi:10.1371/journal.ppat.0030010 · Cited by 311 · see citing works

Identification of an infectious progenitor for the multiple-copy HERV-K human endogenous retroelements

Dewannieux, M., Harper, F., Richaud, A., Letzelter, C., Ribet, D., Pierron, G., & Heidmann, T. (2006) · Genome Research · doi:10.1101/gr.5565706 · Cited by 309 · see citing works

The expanding scope of DNA sequencing

Shendure, J., & Aiden, E. L. (2012) · Nature Biotechnology · doi:10.1038/nbt.2421 · Cited by 300 · see citing works

Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides

Gibson, D. G. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp687 · Cited by 290 · see citing works

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

Warner, J. R., Reeder, P. J., Karimpour‐Fard, A., Woodruff, L. B. A., & Gill, R. T. (2010) · Nature Biotechnology · doi:10.1038/nbt.1653 · Cited by 284 · see citing works

Live attenuated influenza virus vaccines by computer-aided rational design

Mueller, S., Coleman, J. R., Papamichail, D., Ward, C. B., Nimnual, A., Futcher, B., Skiena, S., & Wimmer, E. (2010) · Nature Biotechnology · doi:10.1038/nbt.1636 · Cited by 275 · see citing works

Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast

Agarwal, K. L., Büchi, H., Caruthers, M. H., Gupta, N. K., Khorana, H. G., Kleppe, K., Kumar, A., Ohtsuka, E., RajBhandary, U. L., van de Sande, J. H., et al. (1970) · Nature · doi:10.1038/227027a0 · Cited by 273 · see citing works

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips

Kosuri, S., Eroshenko, N., LeProust, E. M., Super, M., Way, J. C., Li, J. B., & Church, G. M. (2010) · Nature Biotechnology · doi:10.1038/nbt.1716 · Cited by 266 · see citing works

Synthetic recombinant bat SARS-like coronavirus is infectious in cultured cells and in mice

Becker, M. M., Graham, R. L., Donaldson, E., Rockx, B., Sims, A. M., Sheahan, T. P., Pickles, R. J., Corti, D., Johnston, R. E., Baric, R. S., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0808116105 · Cited by 257 · see citing works

Modulation of Poliovirus Replicative Fitness in HeLa Cells by Deoptimization of Synonymous Codon Usage in the Capsid Region

Burns, C. C., Shaw, J., Campagnoli, R. P., Jorba, J. C., Vincent, A., Quay, J., & Kew, O. M. (2006) · Journal of Virology · doi:10.1128/jvi.80.7.3259-3272.2006 · Cited by 257 · see citing works

Rapid and Reliable DNA Assembly via Ligase Cycling Reaction

de Kok, S., Stanton, L. H., Slaby, T., et al. (2014) · ACS Synthetic Biology · doi:10.1021/sb4001992 · Cited by 246 · see citing works

Synthesis of Methyl Halides from Biomass Using Engineered Microbes

Bayer, T. S., Widmaier, D. M., Temme, K., Mirsky, E. A., Santi, D. V., & Voigt, C. A. (2009) · Journal of the American Chemical Society · doi:10.1021/ja809461u · Cited by 241 · see citing works

Massively parallel decoding of mammalian regulatory sequences supports a flexible organizational model

Smith, R. P., Taher, L., Patwardhan, R. P., Kim, M. J., Inoue, F., Shendure, J., Ovcharenko, I., & Ahituv, N. (2013) · Nature Genetics · doi:10.1038/ng.2713 · Cited by 239 · see citing works

Complex effects of nucleotide variants in a mammalian cis -regulatory element

Kwasnieski, J. C., Mogno, I., Myers, C. A., Corbo, J. C., & Cohen, B. A. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1210678109 · Cited by 233 · see citing works

Specific Capture and Whole-Genome Sequencing of Viruses from Clinical Samples

Depledge, D. P., Palser, A. L., Watson, S. J., Lai, I. Y.-C., Gray, E. R., Grant, P. R., Kanda, R. K., LeProust, E. M., Kellam, P., & Breuer, J. (2011) · PLoS ONE · doi:10.1371/journal.pone.0027805 · Cited by 225 · see citing works

Deep mutational scanning of an RRM domain of the Saccharomyces cerevisiae poly(A)-binding protein

Melamed, D., Young, D. L., Gamble, C. E., Miller, C. R., & Fields, S. (2013) · RNA · doi:10.1261/rna.040709.113 · Cited by 223 · see citing works

Deep mutational scanning: assessing protein function on a massive scale

Araya, C. L., & Fowler, D. M. (2011) · Trends in biotechnology · doi:10.1016/j.tibtech.2011.04.003 · Cited by 217 · see citing works

Genome engineering

Carr, P. A., & Church, G. M. (2009) · Nature Biotechnology · doi:10.1038/nbt.1590 · Cited by 203 · see citing works

Quantitative estimation of activity and quality for collections of functional genetic elements

Mutalik, V. K., Guimaraes, J. C., Cambray, G., Mai, Q.-A., Christoffersen, M. J., Martin, L., Yu, A., Lam, C. S. C., Rodríguez, C., Bennett, G., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2403 · Cited by 199 · see citing works

Synthetic Generation of Influenza Vaccine Viruses for Rapid Response to Pandemics

Dormitzer, P. R., Suphaphiphat, P., Gibson, D. G., Wentworth, D. E., Stockwell, T. B., Algire, M. A., Alperovich, N., Barro, M., Brown, D., Craig, S., et al. (2013) · Science Translational Medicine · doi:10.1126/scitranslmed.3006368 · Cited by 196 · see citing works

Massively parallel in vivo enhancer assay reveals that highly local features determine the cis -regulatory function of ChIP-seq peaks

White, M. A., Myers, C. A., Corbo, J. C., & Cohen, B. A. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1307449110 · Cited by 195 · see citing works

Measurement and modeling of intrinsic transcription terminators

Cambray, G., Guimaraes, J. C., Mutalik, V. K., et al. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt163 · Cited by 189 · see citing works

Nucleotides part XXXII. Synthesis of a dithymidine dinucleotide containing a 3′: 5′-internucleotidic linkage

Michelson, A. M., & Todd, A. R. (1955) · Journal of the Chemical Society (Resumed) · doi:10.1039/jr9550002632 · Cited by 174 · see citing works

CombiMatrix oligonucleotide arrays: Genotyping and gene expression assays employing electrochemical detection

Ghindilis, A. L., Smith, M. W., Schwarzkopf, K. R., Roth, K. M., Peyvan, K., Munro, S. B., Lodes, M. J., Stöver, A. G., Bernards, K., Dill, K., et al. (2006) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2006.06.024 · Cited by 170 · see citing works

Genetic inactivation of poliovirus infectivity by increasing the frequencies of CpG and UpA dinucleotides within and across synonymous capsid region codons

Burns, C. C., Campagnoli, R., Shaw, J., Vincent, A., Jorba, J., & Kew, O. (2009) · Journal of Virology · doi:10.1128/jvi.00508-09 · Cited by 170 · see citing works

Design of orthogonal genetic switches based on a crosstalk map of σs, anti‐σs, and promoters

Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Burkhardt, D. H., Clancy, K., Peterson, T. C., Gross, C. A., et al. (2013) · Molecular Systems Biology · doi:10.1038/msb.2013.58 · Cited by 168 · see citing works

Digital RNA allelotyping reveals tissue-specific and allele-specific gene expression in human

Zhang, K., Li, J. B., Gao, Y., Egli, D. M., Xie, B., Deng, J., Li, Z., Lee, J., Aach, J., LeProust, E. M., et al. (2009) · Nature Methods · doi:10.1038/nmeth.1357 · Cited by 156 · see citing works

Parallel on-chip gene synthesis and application to optimization of protein expression

Quan, J., Saaem, I., Tang, N. C., Ma, S., Negre, N. N., GONG, H. R., White, K. P., & Tian, J. (2011) · Nature Biotechnology · doi:10.1038/nbt.1847 · Cited by 152 · see citing works

A flexible light-directed DNA chip synthesis gated by deprotection using solution photogenerated acids

Gao, X. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.22.4744 · Cited by 148 · see citing works

Gene synthesis demystified

Czar, M. J., Anderson, J. C., Bader, J. S., & Peccoud, J. (2008) · Trends in biotechnology · doi:10.1016/j.tibtech.2008.10.007 · Cited by 143 · see citing works

Probing the Limits of Genetic Recoding in Essential Genes

Lajoie, J. G., Kosuri, S., Mosberg, J. A., Gregg, C. J., Zhang, D., & Church, G. M. (2013) · Science · doi:10.1126/science.1241460 · Cited by 140 · see citing works

Protein-mediated error correction for de novo DNA synthesis

Carr, P. A. (2004) · Nucleic Acids Research · doi:10.1093/nar/gnh160 · Cited by 139 · see citing works

Two-step total gene synthesis method

Young, L. (2004) · Nucleic Acids Research · doi:10.1093/nar/gnh058 · Cited by 139 · see citing works

Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes

Ivarsson, Y., Arnold, R., McLaughlin, M., Nim, S., Joshi, R., Ray, D., Liu, B., Teyra, J., Pawson, T., Moffat, J., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1312296111 · Cited by 135 · see citing works

Synthetic design of strong promoters

Schlabach, M. R., Hu, J. K., Li, M. Z., & Elledge, S. J. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914803107 · Cited by 135 · see citing works

Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences

Zhou, X. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh879 · Cited by 134 · see citing works

Synthesis of DNA/RNA and Their Analogs via Phosphoramidite and H-Phosphonate Chemistries

Roy, S., & Caruthers, M. H. (2013) · Molecules · doi:10.3390/molecules181114268 · Cited by 124 · see citing works

Massively parallel synthetic promoter assays reveal the in vivo effects of binding site variantsPreprint

Mogno, I., Kwasnieski, J. C., & Cohen, B. (2013) · Genome Research · doi:10.1101/gr.157891.113 · Cited by 117 · see citing works

Refactored M13 Bacteriophage as a Platform for Tumor Cell Imaging and Drug Delivery

Ghosh, D., Kohli, A. G., Moser, F., et al. (2012) · ACS Synthetic Biology · doi:10.1021/sb300052u · Cited by 114 · see citing works

PhIP-Seq characterization of autoantibodies from patients with multiple sclerosis, type 1 diabetes and rheumatoid arthritis

Larman, H. B., Laserson, U., Querol, L., Verhaeghen, K., Solimini, N. L., Xu, G. J., Klarenbeek, P. L., Church, G. M., Hafler, D. A., Plenge, R. M., et al. (2013) · Journal of Autoimmunity · doi:10.1016/j.jaut.2013.01.013 · Cited by 113 · see citing works

A fully decompressed synthetic bacteriophage øX174 genome assembled and archived in yeast

Jaschke, P. R., Lieberman, E., Rodriguez, J., et al. (2012) · Virology · doi:10.1016/j.virol.2012.09.020 · Cited by 110 · see citing works

Enrichment of sequencing targets from the human genome by solution hybridization

Tewhey, R., Nakano, M., Wang, X., Pabón-Peña, C., Novak, B. A., Giuffre, A. C., Lin, E., Happe, S., Roberts, D. N., LeProust, E. M., et al. (2009) · Genome biology · doi:10.1186/gb-2009-10-10-r116 · Cited by 109 · see citing works

Production of complex nucleic acid libraries using highly parallel in situ oligonucleotide synthesis

Cleary, M. A., Kilian, K. A., Wang, Y., Bradshaw, J., Cavet, G., Ge, W., Kulkarni, A., Paddison, P. J., Chang, K., Sheth, N. U., et al. (2004) · Nature Methods · doi:10.1038/nmeth724 · Cited by 108 · see citing works

Multifactorial Determinants of Protein Expression in Prokaryotic Open Reading Frames

Allert, M. J., Cox, J. C., & Hellinga, H. W. (2010) · Journal of Molecular Biology · doi:10.1016/j.jmb.2010.08.010 · Cited by 106 · see citing works

DNA as a Universal Substrate for Chemical Kinetics

Soloveichik, D., Seelig, G., & Winfree, E. (2009) · Lecture notes in computer science · doi:10.1007/978-3-642-03076-5_6 · Cited by 102 · see citing works

High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing

Matzas, M., Stähler, P. F., Kefer, N., Siebelt, N., Boisguérin, V., Leonard, J. T., Keller, A., Stähler, C., Häberle, P., Gharizadeh, B., et al. (2010) · Nature Biotechnology · doi:10.1038/nbt.1710 · Cited by 96 · see citing works

Kinetic studies on depurination and detritylation of CPG-bound intermediates during oligonucleotide synthesis

Septak, M. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.15.3053 · Cited by 96 · see citing works

Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules

Schwartz, J. J., Lee, C., & Shendure, J. (2012) · Nature Methods · doi:10.1038/nmeth.2137 · Cited by 94 · see citing works

Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis

Richmond, K. E. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh793 · Cited by 90 · see citing works

Correcting errors in synthetic DNA through consensus shuffling

Binkowski, B. F. (2005) · Nucleic Acids Research · doi:10.1093/nar/gni053 · Cited by 84 · see citing works

Gene Synthesis by a LCR-Based Approach: High-Level Production of Leptin-L54 Using Synthetic Gene inEscherichia coli

Au, L., Yang, F., Yang, W., Lo, S.-H., & Kao, C.-F. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.8929 · Cited by 80 · see citing works

Gene synthesis by circular assembly amplification

Bang, D., & Church, G. M. (2007) · Nature Methods · doi:10.1038/nmeth1136 · Cited by 74 · see citing works

644. Nucleotides. Part XLI. Mixed anhydrides as intermediates in the synthesis of dinucleoside phosphates

Hall, R. H., Todd, A. R., & Webb, R. F. (1957) · Journal of the Chemical Society (Resumed) · doi:10.1039/jr9570003291 · Cited by 73 · see citing works

Multiplex padlock targeted sequencing reveals human hypermutable CpG variationsPreprint

Li, J. B., Gao, Y., Aach, J., Zhang, K., Kryukov, G. V., Xie, B., Ahlford, A., Yoon, J.-K., Rosenbaum, A. M., Zaranek, A. W., et al. (2009) · Genome Research · doi:10.1101/gr.092213.109 · Cited by 73 · see citing works

In situ Synthesis of DNA Microarray on Functionalized Cyclic Olefin Copolymer Substrate

Saaem, I., Ma, K.-S., Marchi, A. N., et al. (2010) · ACS Applied Materials & Interfaces · doi:10.1021/am900884b · Cited by 72 · see citing works

Generation of Infectious Molecular Clones of Simian Immunodeficiency Virus from Fecal Consensus Sequences of Wild Chimpanzees

Takehisa, J., Kraus, M. H., Decker, J. M., Li, Y., Keele, B. F., Bibollet‐Ruche, F., Zammit, K. P., Weng, Z., Santiago, M. L., Kamenya, S. M., et al. (2007) · Journal of Virology · doi:10.1128/jvi.00551-07 · Cited by 71 · see citing works

High-quality gene assembly directly from unpurified mixtures of microarray-synthesized oligonucleotides

Borovkov, A. Y., Loskutov, A. V., Robida, M. D., Day, K. M., Cano, J. A., Olson, T. L., Patel, H., Brown, K., Hunter, P. D., & Sykes, K. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq677 · Cited by 68 · see citing works

Chemical gene synthesis: strategies, softwares, error corrections, and applications

Xiong, A. S., Peng, R., Zhuang, J., Gao, F., Li, Y., Cheng, Z., & Yao, Q. (2008) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2008.00109.x · Cited by 65 · see citing works

Programming biological operating systems: genome design, assembly and activation.

Gibson, D. G. (2014) · Nature Methods · doi:10.1038/nmeth.2894 · Cited by 65 · see citing works

High-throughput Analysis of in vivo Protein Stability

Kim, I., Miller, C. R., Young, D. L., & Fields, S. (2013) · Molecular & Cellular Proteomics · doi:10.1074/mcp.o113.031708 · Cited by 65 · see citing works

Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

Fuhrmann, M. (2005) · Nucleic Acids Research · doi:10.1093/nar/gni058 · Cited by 63 · see citing works

Removal of polymerase-produced mutant sequences from PCR products

Smith, J., & Modrich, P. L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.13.6847 · Cited by 62 · see citing works

SYNTHESES OF DIDEOXYRIBONUCLEOTIDES

Khorana, H. G., Razzell, W. E., Gilham, P. T., et al. (1957) · Journal of the American Chemical Society · doi:10.1021/ja01561a065 · Cited by 49 · see citing works

Construction of a rationally designed antibody platform for sequencing-assisted selection

Larman, H. B., Xu, G. J., Pavlova, N. N., & Elledge, S. J. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215549109 · Cited by 41 · see citing works

‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules

Kim, H., Han, H., Ahn, J., Lee, J., Cho, N., Jang, H. S., Kim, H., Kwon, S., & Bang, D. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks546 · Cited by 40 · see citing works

Error correction of microchip synthesized genes using Surveyor nuclease

Saaem, I., Ma, S., Quan, J., et al. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr887 · Cited by 39 · see citing works

Assembling Large DNA Segments in Yeast

Muller, H., Annaluru, N., Schwerzmann, J., et al. (2012) · Methods in molecular biology · doi:10.1007/978-1-61779-564-0_11 · Cited by 39 · see citing works

One-Pot Assembly of a Hetero-dimeric DNA Origami from Chip-Derived Staples and Double-Stranded Scaffold

Marchi, A. N., Saaem, I., Tian, J., & LaBean, T. H. (2013) · ACS Nano · doi:10.1021/nn302322j · Cited by 36 · see citing works

Depurination As a Yield Decreasing Mechanism in Oligodeoxynucleotide Synthesis

Efcavitch, J. W., & Heiner, C. R. (1985) · Nucleosides and Nucleotides · doi:10.1080/07328318508077883 · Cited by 35 · see citing works

Total synthesis of a tyrosine suppressor transfer RNA gene. XVI. Enzymatic joinings to form the total 207-base pair-long DNA.

Sekiya, T., Takeya, T., Brown, E. L., BELAGAJE, R., Contreras, R., Fritz, H., Gait, M. J., Lees, R. G., Ryan, M. J., Khorana, H. G., et al. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50482-8 · Cited by 34 · see citing works

Synthetic Poliovirus and Other Designer Viruses: What Have We Learned from Them?

Wimmer, E., & Paul, A. V. (2010) · Annual Review of Microbiology · doi:10.1146/annurev-micro-090110-102957 · Cited by 33 · see citing works

The rise of a digital immune system

Schatz, M. C., & Phillippy, A. M. (2012) · GigaScience · doi:10.1186/2047-217x-1-4 · Cited by 32 · see citing works

Targeted genome enrichment for efficient purification of endosymbiont DNA from host DNA

Geniez, S., Foster, J. M., Kumar, S., Moumen, B., LeProust, E. M., Hardy, O., Guadalupe, M., Thomas, S. J., Boone, B. E., Hendrickson, C. L., et al. (2012) · Symbiosis · doi:10.1007/s13199-012-0215-x · Cited by 28 · see citing works

An inactivated West Nile Virus vaccine derived from a chemically synthesized cDNA system

Orlinger, K. K., Holzer, G. W., Schwaiger, J., Mayrhofer, J., Schmid, K., Kistner, O., Barrett, P. N., & Falkner, F. G. (2010) · Vaccine · doi:10.1016/j.vaccine.2010.02.092 · Cited by 26 · see citing works

Oligonucleotide Assembly in Yeast to Produce Synthetic DNA Fragments

Gibson, D. G. (2012) · Methods in molecular biology · doi:10.1007/978-1-61779-564-0_2 · Cited by 24 · see citing works

1-induced DNA bending is required for transcriptional activation by the Mcm1- 1 complex

Carr, E. A. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh560 · Cited by 23 · see citing works

Whole-Genome Synthesis and Characterization of Viable S13-Like Bacteriophages

Liu, Y., Han, Y., Huang, W., Duan, Y., Mou, L., Jiang, Z., Fa, P., Xie, J., Diao, R., Chen, Y., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0041124 · Cited by 20 · see citing works

New Tools for Cost-Effective DNA Synthesis

Tang, N. C., Ma, S., & Tian, J. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-394430-6.00001-7 · Cited by 15 · see citing works

A Fluorescence Selection Method for Accurate Large‐Gene Synthesis

Kim, H., Han, H., Shin, D., et al. (2010) · ChemBioChem · doi:10.1002/cbic.201000368 · Cited by 13 · see citing works

When It Rains, It Pores

Kosuri, S., & Sismour, A. M. (2012) · ACS Synthetic Biology · doi:10.1021/sb300015f · Cited by 2 · see citing works

DNA nanotechnology

Seeman, N. C., & Sleiman, H. F. (2017) · Nature Reviews Materials · doi:10.1038/natrevmats.2017.68 · Cited by 1,875 · see citing works

DNA Fountain enables a robust and efficient storage architecture

Erlich, Y., & Zielinski, D. (2017) · Science · doi:10.1126/science.aaj2038 · Cited by 898 · see citing works

Automating drug discovery

Schneider, G. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2017.232 · Cited by 910 · see citing works

Robust Chemical Preservation of Digital Information on DNA in Silica with Error-Correcting Codes

Grass, R. N., Heckel, R., Puddu, M., Paunescu, D., & Stark, W. J. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201411378 · Cited by 754 · see citing works

Random access in large-scale DNA data storage

Organick, L., Ang, S. D., Chen, Y.-J., Lopez, R., Yekhanin, S., Makarychev, K., Racz, M. Z., Kamath, G., Gopalan, P., Nguyen, B., et al. (2018) · Nature Biotechnology · doi:10.1038/nbt.4079 · Cited by 775 · see citing works

Global analysis of protein folding using massively parallel design, synthesis, and testing

Rocklin, G. J., Chidyausiku, T. M., Goreshnik, I., Ford, A., Houliston, S., Lemak, A., Carter, L., Ravichandran, R., Mulligan, V. K., Chevalier, A., et al. (2017) · Science · doi:10.1126/science.aan0693 · Cited by 501 · see citing works

Molecular Digital Data Storage Using DNA

Ceze, L., Nivala, J., & Strauss, K. (2019) · Nature Reviews Genetics · doi:10.1038/s41576-019-0125-3 · Cited by 551 · see citing works

Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology

Hughes, R. A., & Ellington, A. D. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a023812 · Cited by 411 · see citing works

De novo DNA synthesis using polymerase-nucleotide conjugates.

Palluk, S., Arlow, D. H., de Rond, T., Barthel, S., Kang, J. S., Bector, R., Baghdassarian, H. M., Truong, A. N., Kim, P. W., Singh, A. K., et al. (2018) · Nature Biotechnology · doi:10.1038/nbt.4173 · Cited by 316 · see citing works

Genomically encoded analog memory with precise in vivo DNA writing in living cell populations

Farzadfard, F., & Lu, T. K. (2014) · Science · doi:10.1126/science.1256272 · Cited by 298 · see citing works

Bricks and blueprints: methods and standards for DNA assembly.

Casini, A., Storch, M., Baldwin, G. S., & Ellis, T. (2015) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm4014 · Cited by 286 · see citing works

DNA-Based Storage: Trends and Methods

Yazdi, S. M. H. T., Kiah, H. M., Garcia-Ruiz, E., Ma, J., Zhao, H., & Milenkovic, O. (2015) · IEEE Transactions on Molecular, Biological and Multi-Scale Communications · doi:10.1109/tmbmc.2016.2537305 · Cited by 278 · see citing works

Emerging Approaches to DNA Data Storage: Challenges and Prospects

Doricchi, A., Platnich, C. M., Gimpel, A., Horn, F., Earle, M., Lanzavecchia, G., Cortajarena, A. L., Liz-Marzán, L. M., Liu, N., Heckel, R., et al. (2022) · ACS Nano · doi:10.1021/acsnano.2c06748 · Cited by 235 · see citing works

Data storage in DNA with fewer synthesis cycles using composite DNA letters.

Anavy, L., Vaknin, I., Atar, O., Amit, R., & Yakhini, Z. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0240-x · Cited by 229 · see citing works

Encoding information into polymers

Rutten, M. G. T. A., Vaandrager, F. W., Elemans, J. A. A. W., & Nolte, R. J. M. (2018) · Nature Reviews Chemistry · doi:10.1038/s41570-018-0051-5 · Cited by 216 · see citing works

Reading and Writing Digital Data in DNA

Meiser, L. C., Antkowiak, P. L., Koch, J., Chen, W. D., Kohll, A. X., Stark, W. J., Heckel, R., & Grass, R. N. (2020) · Nature Protocols · doi:10.1038/s41596-019-0244-5 · Cited by 164 · see citing works

Random access DNA memory using Boolean search in an archival file storage system

Banal, J. L., Shepherd, T. R., Berleant, J., Huang, H., Reyes, M., Ackerman, C. M., Blainey, P. C., & Bathe, M. (2021) · Nature Materials · doi:10.1038/s41563-021-01021-3 · Cited by 143 · see citing works

Demonstration of End-to-End Automation of DNA Data Storage.

Takahashi, C. N., Nguyen, B. H., Strauss, K., & Ceze, L. (2019) · Scientific Reports · doi:10.1038/s41598-019-41228-8 · Cited by 137 · see citing works

High information capacity DNA-based data storage with augmented encoding characters using degenerate bases

Choi, Y., Ryu, T., Lee, A. C., Choi, H., Lee, H., Park, J., Song, S.-H., Kim, S., Kim, H., Park, W., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-43105-w · Cited by 128 · see citing works

Quantifying Molecular Bias in DNA Data Storage

Chen, Y.-J., Takahashi, C. N., Organick, L., Stewart, K., Ang, S. D., Weiss, P., Peck, B., Seelig, G., Ceze, L., & Strauss, K. (2019) · doi:10.1101/566554 · Cited by 109 · see citing works

Template-Independent Enzymatic Oligonucleotide Synthesis (TiEOS): Its History, Prospects, and Challenges

Jensen, M. A., & Davis, R. W. (2018) · Biochemistry · doi:10.1021/acs.biochem.7b00937 · Cited by 106 · see citing works

Combining Data Longevity with High Storage Capacity—Layer‐by‐Layer DNA Encapsulated in Magnetic Nanoparticles

Chen, W. D., Kohll, A. X., Nguyen, B. H., Koch, J., Heckel, R., Stark, W. J., Ceze, L., Strauss, K., & Grass, R. N. (2019) · Advanced Functional Materials · doi:10.1002/adfm.201901672 · Cited by 104 · see citing works

Data Storage Based on DNA

Hao, Y., Li, Q., Fan, C., & Wang, F. (2021) · Small Structures · doi:10.1002/sstr.202000046 · Cited by 90 · see citing works

Scaling DNA data storage with nanoscale electrode wells

Nguyen, B. H., Takahashi, C. N., Gupta, G., Smith, J. A., Rouse, R., Berndt, P., Yekhanin, S., Ward, D. P., Ang, S. D., Garvan, P., et al. (2021) · Science Advances · doi:10.1126/sciadv.abi6714 · Cited by 90 · see citing works

From genotypes to organisms: State-of-the-art and perspectives of a cornerstone in evolutionary dynamics

Manrubia, S., Cuesta, J. A., Aguirre, J., Ahnert, S. E., Altenberg, L., Cano, A. V., Catalán, P., Diaz-Uriarte, R., Elena, S. F., García-Martín, J. A., et al. (2021) · Physics of Life Reviews · doi:10.1016/j.plrev.2021.03.004 · Cited by 88 · see citing works

A DNA-Based Archival Storage System

Bornholt, J., Lopez, R., Carmean, D. M., Ceze, L., Seelig, G., & Strauss, K. (2016) · ACM SIGOPS Operating Systems Review · doi:10.1145/2954680.2872397 · Cited by 77 · see citing works

Large-Scale de Novo Oligonucleotide Synthesis for Whole-Genome Synthesis and Data Storage: Challenges and Opportunities

Song, L.-F., Deng, Z.-H., Gong, Z.-Y., Li, L.-L., & Li, B.-Z. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.689797 · Cited by 74 · see citing works

DNA Data Storage and Hybrid Molecular–Electronic Computing

Carmean, D., Ceze, L., Seelig, G., Stewart, K., Strauss, K., & Willsey, M. (2019) · Proceedings of the IEEE · doi:10.1109/jproc.2018.2875386 · Cited by 69 · see citing works

Information-Theoretic Foundations of DNA Data Storage

Shomorony, I., & Heckel, R. (2022) · Foundations and Trends® in Communications and Information Theory · doi:10.1561/0100000117 · Cited by 62 · see citing works

Carbon-based archiving: current progress and future prospects of DNA-based data storage.

Ping, Z., Ma, D., Huang, X., Chen, S., Liu, L., Guo, F., Zhu, S. J., & Shen, Y. (2019) · GigaScience · doi:10.1093/gigascience/giz075 · Cited by 55 · see citing works

A DNA-Based Archival Storage System

Bornholt, J., Lopez, R., Carmean, D. M., Ceze, L., Seelig, G., & Strauss, K. (2016) · ACM SIGPLAN Notices · doi:10.1145/2954679.2872397 · Cited by 54 · see citing works

Systems and synthetic biology approaches to alter plant cell walls and reduce biomass recalcitrance

Kalluri, U. C., Yin, H., Yang, X., & Davison, B. H. (2014) · Plant Biotechnology Journal · doi:10.1111/pbi.12283 · Cited by 54 · see citing works

DNA synthesis for true random number generation

Meiser, L. C., Koch, J., Antkowiak, P. L., Stark, W. J., Heckel, R., & Grass, R. N. (2020) · Nature Communications · doi:10.1038/s41467-020-19757-y · Cited by 45 · see citing works

A Mixed Culture of Bacterial Cells Enables an Economic DNA Storage on a Large Scale

Hao, M., Qiao, H., Gao, Y., Wang, Z., Qiao, X., Chen, X., & Qi, H. (2020) · Communications Biology · doi:10.1038/s42003-020-01141-7 · Cited by 41 · see citing works

Protein engineering by highly parallel screening of computationally designed variants

Sun, M. G. F., Seo, M.-H., Nim, S., Corbi-Verge, C., & Kim, P. M. (2016) · Science Advances · doi:10.1126/sciadv.1600692 · Cited by 41 · see citing works

Reading and Writing Digital Information in TNA

Yang, K., McCloskey, C. M., & Chaput, J. C. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.0c00361 · Cited by 41 · see citing works

Purification of multiplex oligonucleotide libraries by synthesis and selection

Choi, H., Choi, Y., Choi, J., Lee, A. C., Yeom, H., Hyun, J., Ryu, T., & Kwon, S. (2022) · Nature Biotechnology · doi:10.1038/s41587-021-00988-3 · Cited by 32 · see citing works

Combinatorial PCR Method for Efficient, Selective Oligo Retrieval from Complex Oligo Pools

Winston, C., Organick, L., Ward, D., Ceze, L., Strauss, K., & Chen, Y.-J. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.1c00482 · Cited by 29 · see citing works

Preservation and Encryption in DNA Digital Data Storage

Zhang, Y., Ren, Y., Liu, Y., Wang, F., Zhang, H., & Liu, K. (2022) · ChemPlusChem · doi:10.1002/cplu.202200183 · Cited by 28 · see citing works

An Epigenetics‐Inspired DNA‐Based Data Storage System

Mayer, C., McInroy, G. R., Murat, P., Van Delft, P., & Balasubramanian, S. (2016) · Angewandte Chemie · doi:10.1002/ange.201605531 · Cited by 19 · see citing works

Application of Array-Based Oligonucleotides for Synthesis of Genetic Designs

Sinyakov, A. N., Ryabinin, V. A., & Kostina, E. V. (2021) · Molecular Biology · doi:10.1134/s0026893321030109 · Cited by 9 · see citing works

A Characterization of the DNA Data Storage Channel

Heckel, R., Mikutis, G., & Grass, R. N. (2019) · Scientific Reports · doi:10.1038/s41598-019-45832-6 · Cited by 9 · see citing works

Sampling of Structure and Sequence Space of Small Protein Folds

Linsky, T., Noble, K., Tobin, A., Crow, R., Carter, L., Urbauer, J., Baker, D., & Strauch, E. (2021) · doi:10.1101/2021.03.10.434454

Leveraging pre-trained AI models for robust promoter sequence design in synthetic biology

Yang, G., Chen, Y., Guo, Q., Li, X., & Zhou, Z. (2026) · Biophysics Reports · doi:10.52601/bpr.2025.240033 · Cited by 3 · see citing works

DNA as a Data Storage Medium.

Rebimbas, R., Glória, I., Chegão, J., Al-Rawi, M., Mousakhani Ganjeh, A., & Saraiva, J. A. (2026) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2026.03.003

The promise of synthetic biology

Pleiss, J. (2006) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0664-3 · Cited by 85 · see citing works

Special focus: Synthetic biology

(2009) · Biotechnology Journal · doi:10.1002/biot.200990087

Synthetic biology : tools and applications

趙, 会. (2013) · Elsevier eBooks · Cited by 5 · see citing works

Advancing synthetic biology through cell-free protein synthesis

Yue, K., Chen, J., Li, Y., & Kai, L. (2023) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2023.05.003 · Cited by 42 · see citing works

Plant synthetic biology

Liu, W., & Stewart, C. N. (2015) · Trends in Plant Science · doi:10.1016/j.tplants.2015.02.004 · Cited by 212 · see citing works