The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Open accessAbstract
All-optical electrophysiology—spatially resolved simultaneous optical perturbation and measurement of membrane voltage—would open new vistas in neuroscience research. We evolved two archaerhodopsin-based voltage indicators, QuasAr1 and QuasAr2, which show improved brightness and voltage sensitivity, have microsecond response times and produce no photocurrent. We engineered a channelrhodopsin actuator, CheRiff, which shows high light sensitivity and rapid kinetics and is spectrally orthogonal to the QuasArs. A coexpression vector, Optopatch, enabled cross-talk–free genetically targeted all-optical electrophysiology. In cultured rat neurons, we combined Optopatch with patterned optical excitation to probe back-propagating action potentials (APs) in dendritic spines, synaptic transmission, subcellular microsecond-timescale details of AP propagation, and simultaneous firing of many neurons in a network. Optopatch measurements revealed homeostatic tuning of intrinsic excitability in human stem cell–derived neurons. In rat brain slices, Optopatch induced and reported APs and subthreshold events with high signal-to-noise ratios. The Optopatch platform enables high-throughput, spatially resolved electrophysiology without the use of conventional electrodes.
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Independent optical excitation of distinct neural populations
Klapoetke, N. C., Murata, Y., Kim, S. S., Pulver, S. R., Birdsey-Benson, A., Cho, Y. K., Morimoto, T. K., Chuong, A. S., Carpenter, E. J., Tian, Z., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2836 · Cited by 2,344
Self-citationThe subcellular organization of neocortical excitatory connections
Petreanu, L., Mao, T., Sternson, S. M., & Svoboda, K. (2009) · Nature · doi:10.1038/nature07709 · Cited by 1,047
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SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients
Shcheglovitov, A., Shcheglovitova, O., Yazawa, M., Portmann, T., Shu, R., Sebastiano, V., Krawisz, A., Froehlich, W., Bernstein, J. A., Hallmayer, J. F., et al. (2013) · Nature · doi:10.1038/nature12618 · Cited by 470
Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein
Akemann, W., Mutoh, H., Perron, A., Park, Y. K., Iwamoto, Y., & Knöpfel, T. (2012) · Journal of Neurophysiology · doi:10.1152/jn.00452.2012 · Cited by 237
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Novel electrode technologies for neural recordings
Hong, G., & Lieber, C. M. (2019) · Nature Reviews Neuroscience · doi:10.1038/s41583-019-0140-6 · Cited by 695
High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor
Gong, Y., Huang, C., Li, J. Z., Grewe, B. F., Zhang, Y., Eismann, S., & Schnitzer, M. J. (2015) · Science · doi:10.1126/science.aab0810 · Cited by 462
Different Neuronal Activity Patterns Induce Different Gene Expression Programs
Tyssowski, K. M., DeStefino, N. R., Cho, J.-H., Dunn, C. J., Poston, R. G., Carty, C. E., Jones, R. D., Chang, S. M., Romeo, P., Wurzelmann, M. K., et al. (2018) · Neuron · doi:10.1016/j.neuron.2018.04.001 · Cited by 390
Shi, Z., Graber, Z. T., Baumgart, T., Stone, H. A., & Cohen, A. E. (2018) · Cell · doi:10.1016/j.cell.2018.09.054 · Cited by 355
Self-citationThe promises and challenges of human brain organoids as models of neuropsychiatric disease
Quadrato, G., Brown, J., & Arlotta, P. (2016) · Nature Medicine · doi:10.1038/nm.4214 · Cited by 304
Metadata
- Authors
- Hochbaum, D. R., Zhao, Y., Farhi, S. L., Klapoetke, N., Werley, C. A., Kapoor, V., Zou, P., Kralj, J. M., Maclaurin, D., Smedemark-Margulies, N., Saulnier, J. L., Boulting, G. L., Straub, C., Cho, Y. K., Melkonian, M., Wong, G. K.-S., Harrison, D. J., Murthy, V. N., Sabatini, B. L., Boyden, E. S., Campbell, R. E., & Cohen, A. E.
- Venue
- Nature Methods
- Published
- 2014-06-22
- Volume
- 11
- Issue
- 8
- Pages
- 825-833
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2145909957
- Cited by
- 770
- References
- 5
- FWCI
- 29.79
- Type
- journal article
- Language
- en
- Open access
- bronze
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