The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs
Open accessAbstract
We present tRNA-based vectors for producing multiple clustered regularly interspaced short palindromic repeats (CRISPR) single guide RNAs (sgRNAs) from a single RNA polymerase II or III transcript in Drosophila. The system, which is based on liberation of sgRNAs by processing flanking tRNAs, permits highly efficient multiplexing of Cas9-based mutagenesis. We also demonstrate that the tRNA–sgRNA system markedly increases the efficacy of conditional gene disruption by Cas9 and can promote editing by the recently discovered RNA-guided endonuclease Cpf1.
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Metadata
- Authors
- Port, F., & Bullock, S. L.
- Venue
- Nature Methods
- Published
- 2016-09-05
- Volume
- 13
- Issue
- 10
- Pages
- 852-854
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2515623196
- Cited by
- 437
- References
- 32
- FWCI
- 16.85
- Type
- journal article
- Language
- en
- Open access
- green
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