In vivo high-throughput profiling of CRISPR-Cpf1 activity

Cited by (384)

Items 101–200 of 384

1. Target-dependent nickase activities of the CRISPR–Cas nucleases Cpf1 and Cas9
   Becky Xu Hua Fu, Justin Smith, Ryan T. Fuchs, Megumu Yamada Mabuchi, Jennifer L. Curcuru, G. Brett Robb, Andrew Z. Fire · 2019 · Nature Microbiology
2. Multiplexed conditional genome editing with Cas12a in Drosophila
   Fillip Port, Maja Starostecka, Michael Boutros · 2020 · Proceedings of the National Academy of Sciences
3. CRISPR-Local: a local single-guide RNA (sgRNA) design tool for non-reference plant genomes
   Jiamin Sun, Hao Liu, Jianxiao Liu, Shikun Cheng, Yong Peng, Qinghua Zhang, Jianbing Yan, Haijun Liu, Ling‐Ling Chen · 2018 · Bioinformatics
4. MnO 2 Nanozyme-Mediated CRISPR-Cas12a System for the Detection of SARS-CoV-2
   Lina Wu, Xinjie Wang, Xiangchuan Wu, Shiqi Xu, Ming Liu, Xizhong Cao, Taishan Tang, Xingxu Huang, He Huang · 2022 · ACS Applied Materials & Interfaces
5. Expanding the CRISPR Toolbox with ErCas12a in Zebrafish and Human Cells
   Wesley A. Wierson, Brandon W. Simone, Zachary WareJoncas, Carla M. Mann, Jordan M. Welker, Bibekananda Kar, Michael J. Emch, Iddo Friedberg, William A.C. Gendron, Michael A. Barry et al. · 2019 · The CRISPR Journal
6. Determinants of CRISPR Cas12a nuclease activation by DNA and RNA targets
   Eric A. Nalefski, Remy M. Kooistra, Ishira Parikh, Samantha Hedley, Karunya Rajaraman, Damian Madan · 2024 · Nucleic Acids Research
7. Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi
   M. Kwon, T. Schütze, Sebastian Spohner, S. Haefner, V. Meyer · 2019 · Fungal Biology and Biotechnology
8. Prediction of CRISPR/Cas9 single guide RNA cleavage efficiency and specificity by attention-based convolutional neural networks
   Guishan Zhang, Tian Zeng, Zhiming Dai, Xianhua Dai · 2021 · Computational and Structural Biotechnology Journal
9. The Conspicuity of CRISPR-Cpf1 System as a Significant Breakthrough in Genome Editing
   Hadi Bayat, Mohammad Hossein Modarressi, Azam Rahimpour · 2017 · Current Microbiology
10. Construction of non-canonical PAM-targeting adenosine base editors by restriction enzyme-free DNA cloning using CRISPR-Cas9
    You Kyeong Jeong, Jihyeon Yu, Sangsu Bae · 2019 · Scientific Reports
11. CRISPR/Cas-based CAR-T cells: production and application
    Ping Song, Qiqi Zhang, Zhiyong Xu, Yueli Shi, Ruirui Jing, Dingcun Luo · 2024 · Biomarker Research
12. How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing
    Yannik Bollen, Jasmin B. Post, Bon‐Kyoung Koo, Hugo J.G. Snippert · 2018 · Nucleic Acids Research
13. Tissue-Specific Delivery of CRISPR Therapeutics: Strategies and Mechanisms of Non-Viral Vectors
    Karim E. Shalaby, Mustapha Aouida, Omar M. A. El‐Agnaf · 2020 · International Journal of Molecular Sciences
14. Design and assessment of engineered CRISPR–Cpf1 and its use for genome editing
    Bin Li, Chunxi Zeng, Yizhou Dong · 2018 · Nature Protocols
15. Prediction of activity and specificity of CRISPR-Cpf1 using convolutional deep learning neural networks
    Jiesi Luo, Wei Chen, Li Xue, Bin Tang · 2019 · BMC Bioinformatics
16. Recent advances of Cas12a applications in bacteria
    Meliawati Meliawati, Christoph Schilling, Jochen Schmid · 2021 · Applied Microbiology and Biotechnology
17. PCDetection: PolyA-CRISPR/Cas12a-based miRNA detection without PAM restriction
    Mingtian Zhong, Kaizhao Chen, Wenjun Sun, Xiangyang Li, Shisheng Huang, Qingzhou Meng, Bo Sun, Xingxu Huang, Xinjie Wang, Xiaodong Ma et al. · 2022 · Biosensors and Bioelectronics
18. Optimized CRISPR-Cpf1 system for genome editing in zebrafish
    Juan Pablo Fernández, Charles E. Vejnar, Antonio J. Giráldez, Romain Rouet, Miguel A. Moreno-Mateos · 2018 · Methods
19. A Novel Hybrid CNN-SVR for CRISPR/Cas9 Guide RNA Activity Prediction
    Guishan Zhang, Zhiming Dai, Xianhua Dai · 2020 · Frontiers in Genetics
20. Multiplex genome editing in Ashbya gossypii using CRISPR-Cpf1
    Alberto Jiménez, Birgit Hoff, José Luis Revuelta · 2020 · New Biotechnology
21. Identification of pathogenic variants in cancer genes using base editing screens with editing efficiency correction
    Changcai Huang, Guangyu Li, Jiayu Wu, Junbo Liang, Xiaoyue Wang · 2021 · Genome biology
22. Terminal deoxynucleotidyl transferase induced activators to unlock the trans-cleavage of CRISPR/Cpf 1 (TdT-IU- CRISPR/Cpf 1): An ultrasensitive biosensor for Dam MTase activity detection
    Xiaolong Chen, Gaihua Cao, Xianfeng Wang, Zhong Ji, Faliang Xu, Danqun Huo, Xiaogang Luo, Changjun Hou · 2020 · Biosensors and Bioelectronics
23. CRISPR activation screens: navigating technologies and applications
    Teleri Clark, Matthew A. Waller, Lipin Loo, Cesar L. Moreno, Christopher E. Denes, G. Gregory Neely · 2024 · Trends in biotechnology
24. Creating a Modular Activatable CRISPR–Cas12a System by Engineering crRNA Scaffold with a Steric Hindrance Effector
    Pengfei Liu, Shengli Li, Jiayu Zeng, Meixia Wang, Jinlian Du, Hong‐Hui Wang, Erhu Xiong, Ronghua Yang · 2025 · Journal of the American Chemical Society
25. The Off-Target Effect of CRISPR-Cas12a System toward Insertions and Deletions between Target DNA and crRNA Sequences
    Wei Zhang, Rui Shi, Kejun Dong, Hao Hu, Wan Shu, Yaoqin Mu, Bei Yan, Longjie Li, Xianjin Xiao, Hongbo Wang · 2022 · Analytical Chemistry
26. CRISPR/Cas-Based Diagnostics in Agricultural Applications
    Tanzena Tanny, Mohamed Sallam, Narshone Soda, Nam‐Trung Nguyen, Mobashwer M. Alam, Muhammad J. A. Shiddiky · 2023 · Journal of Agricultural and Food Chemistry
27. Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition
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28. One‐Pot Isothermal Nucleic Acid Amplification Assisted CRISPR/Cas Detection Technology: Challenges, Strategies, and Perspectives
    Hongyan Liu, Hui Yan Yin, Leshan Xiu, Wenyu Wu, Qinqin Hu, Yingchen Xia, Brianna Garcia, Shamsuttiyeba Shifa, Hui Chen, Min Li et al. · 2025 · Advanced Science
29. Pooled library screening with multiplexed Cpf1 library
    Jintan Liu, Sanjana Srinivasan, Chieh-Yuan Li, I-Lin Ho, Johnathon Lynn Rose, MennatAllah Shaheen, Gang Wang, Wantong Yao, Angela K. Deem, Chris Bristow et al. · 2019 · Nature Communications
30. Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1
    Jiyeon Kweon, An-Hee Jang, Daeun Kim, Jin Wook Yang, Mi-jung Yoon, Ha Rim Shin, Jin‐Soo Kim, Yongsub Kim · 2017 · Nature Communications
31. CRISPR–Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference
    Yu Zhao, Jef D. Boeke · 2020 · Nucleic Acids Research
32. A more efficient CRISPR-Cas12a variant derived from Lachnospiraceae bacterium MA2020
    Mai Hue Tran, HaJeung Park, Christopher L. Nobles, Pabalu P. Karunadharma, Li Pan, Guocai Zhong, Haimin Wang, Wenhui He, Tianling Ou, Gogce C. Crynen et al. · 2021 · Molecular Therapy — Nucleic Acids
33. An integrated CRISPR Bombyx mori genome editing system with improved efficiency and expanded target sites
    Sanyuan Ma, Yue Liu, Yuanyuan Liu, Jiasong Chang, Tong Zhang, Xiaogang Wang, Run Shi, Wei Lü, Xiaojuan Xia, Ping Zhao et al. · 2017 · Insect Biochemistry and Molecular Biology
34. Pairwise library screen systematically interrogates Staphylococcus aureus Cas9 specificity in human cells
    Josh Tycko, Luis Alberto Barrera, Nicholas C. Huston, Ari E. Friedland, Xuebing Wu, Jonathan S. Gootenberg, Omar O. Abudayyeh, Vic E. Myer, Christopher J. Wilson, Patrick D. Hsu · 2018 · Nature Communications
35. Recording of elapsed time and temporal information about biological events using Cas9
    Jihye Park, Jung Min Lim, Inkyung Jung, Seok‐Jae Heo, Jinman Park, Yoo Jin Chang, Hui Kwon Kim, Dongmin Jung, Ji Hea Yu, Seonwoo Min et al. · 2021 · Cell
36. RPA-CRISPR/Cas12a mediated isothermal amplification for visual detection of Phytophthora sojae
    Yufang Guo, Hongming Xia, Tingting Dai, Tingli Liu · 2023 · Frontiers in Cellular and Infection Microbiology
37. The extent of multiallelic, co‐editing of LIGULELESS1 in highly polyploid sugarcane tunes leaf inclination angle and enables selection of the ideotype for biomass yield
    Eleanor J. Brant, Ayman Eid, Baskaran Kannan, Mehmet Cengiz Baloğlu, Fredy Altpeter · 2024 · Plant Biotechnology Journal
38. Allele-specific genome targeting in the development of precision medicine
    Junjiao Wu, Beisha Tang, Yu Tang · 2020 · Theranostics
39. Amplification-Free Electrochemiluminescent Biosensor for Ultrasensitive Detection of Fusobacterium nucleatum Using Tetrahedral DNA-Based CRISPR/Cas12a
    Xindan Zhang, Minkang Wu, Haoran Shi, Soochan Kim, Shixiang Lü, Ping Wang, Jieling Qin · 2025 · Cyborg and Bionic Systems
40. Gene Editing With TALEN and CRISPR/Cas in Rice
    Honghao Bi, Bing Yang · 2017 · Progress in molecular biology and translational science
41. Systematically investigating the key features of the DNase deactivated Cpf1 for tunable transcription regulation in prokaryotic cells
    Chensi Miao, Huiwei Zhao, Long Qian, Chunbo Lou · 2018 · Synthetic and Systems Biotechnology
42. The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants
    Marta Vázquez‐Vilar, Victor García‐Carpintero, Sara Selma, Joan Miquel Bernabé‐Orts, Javier Sánchez-Vicente, Blanca Salazar‐Sarasua, Arianna Ressa, Carmine de Paola, Maria Ajenjo-Bauza, José Carlos Quintela et al. · 2021 · Frontiers in Plant Science
43. Development of an MSPQC Nucleic Acid Sensor Based on CRISPR/Cas9 for the Detection of Mycobacterium tuberculosis
    Ji Huang, Zi Liang, Yu Liu, Jiandang Zhou, Fengjiao He · 2022 · Analytical Chemistry
44. Ultrasensitive SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode
    Lina Wu, Xinjie Wang, Chengyuan Wu, Xizhong Cao, Taishan Tang, He Huang, Xingxu Huang · 2022 · Analytica Chimica Acta
45. Novel Prokaryotic CRISPR-Cas12a-Based Tool for Programmable Transcriptional Activation and Repression
    Christoph Schilling, Mattheos A. G. Koffas, Volker Sieber, Jochen Schmid · 2020 · ACS Synthetic Biology
46. A Cas12a‐based gene editing system for Phytophthora infestans reveals monoallelic expression of an elicitor
    Audrey M. V. Ah‐Fong, Amy M. Boyd, Michael E. H. Matson, Howard S. Judelson · 2021 · Molecular Plant Pathology
47. Advances in Field Detection Based on CRISPR/Cas System
    Mingzhu Yuan, Ronghua Ding, Shuaiyin Chen, Guangcai Duan · 2021 · ACS Synthetic Biology
48. Current advances of CRISPR-Cas technology in cell therapy
    Hou-Yuan Qiu, Rui-Jin Ji, Ying Zhang · 2022 · Cell Insight
49. Computational design of CRISPR guide RNAs to enable strain-specific control of microbial consortia
    Austin G. Rottinghaus, Scott Vo, Tae Seok Moon · 2022 · Proceedings of the National Academy of Sciences
50. Functional interrogation of non-coding DNA through CRISPR genome editing
    Matthew C. Canver, Daniel E. Bauer, Stuart H. Orkin · 2017 · Methods
51. Toward a CRISPR-Based Point-of-Care Test for Tomato Brown Rugose Fruit Virus Detection
    Joan Miquel Bernabé‐Orts, Yolanda Hernando, Miguel A. Aranda · 2021 · PhytoFrontiers™
52. CRISPR applications in cancer diagnosis and treatment
    Mingxia Wang, Menghui Chen, Xia Wu, Xinbo Huang, Bo Yu · 2023 · Cellular & Molecular Biology Letters
53. An amplification-free CRISPR-SERS biosensor for specific, sensitive and rapid detection of Salmonella Typhimurium in poultry
    Fei Jia, Bingyan Li, Yawen He, Yafang Shen, Juhong Chen, Xingmin Li, Yanbin Li · 2023 · LWT
54. A CRISPR/Cas12a-Based System for Sensitive Detection of Antimicrobial-Resistant Genes in Carbapenem-Resistant Enterobacterales
    Jiyong Shin, Sei Rim Kim, Zifan Xie, Yong‐Su Jin, Yi‐Cheng Wang · 2024 · Biosensors
55. Harnessing artificial intelligence to advance CRISPR-based genome editing technologies
    Tyler Thomson, Gen Lin Li, Amy W. Strilchuk, Haotian Cui, Bo Wang, Bowen Li · 2025 · Nature Reviews Genetics
56. Targeted genome editing in algae using CRISPR/Cas9
    Amita Tanwar, Surbhi Sharma, Shashi Bhusan Kumar · 2018 · Indian Journal of Plant Physiology
57. Deep sampling of gRNA in the human genome and deep-learning-informed prediction of gRNA activities
    Heng Zhang, Jianfeng Yan, Zhike Lu, Yangfan Zhou, Qingfeng Zhang, Tingting Cui, Yini Li, Hui Chen, Lijia Ma · 2023 · Cell Discovery
58. CRISPR-Cpf1-mediated genome editing and gene regulation in human cells
    Tianwen Li, Linwen Zhu, Bingxiu Xiao, Zhaohui Gong, Qi Liao, Junming Guo · 2018 · Biotechnology Advances
59. TransCrispr: Transformer Based Hybrid Model for Predicting CRISPR/Cas9 Single Guide RNA Cleavage Efficiency
    Yun-Qi Wan, Zhenran Jiang · 2022 · IEEE Transactions on Computational Biology and Bioinformatics
60. Engineering CRISPR/Cas12-based biosensors: Recent advances and future perspectives
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61. Engineered CRISPR Systems for Next Generation Gene Therapies
    Michael Pineda, Farzaneh Moghadam, Mo R. Ebrahimkhani, Samira Kiani · 2017 · ACS Synthetic Biology
62. A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in Escherichia coli
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63. A modular strategy for extracellular vesicle-mediated CRISPR-Cas9 delivery through aptamer-based loading and UV-activated cargo release
    Omnia M. Elsharkasy, Charlotte V. Hegeman, Tom A. P. Driedonks, Xiuming Liang, Ivana Lansweers, Olaf L. Cotugno, Ingmar Y. de Groot, Zoë E. M. N. J. de Wit, Antonio Garcia-Guerra, Niels J. A. Moorman et al. · 2025 · Nature Communications
64. Genetic engineering of host organisms for pharmaceutical synthesis
    Joseph H Collins, Eric M. Young · 2018 · Current Opinion in Biotechnology
65. Recombinase Polymerase Amplification/Cas12a-Based Identification of Xanthomonas arboricola pv. pruni on Peach
    Mei Luo, Fan-Zhu Meng, Qin Tan, Weixiao Yin, Chaoxi Luo · 2021 · Frontiers in Plant Science
66. From Code to Life: The AI‐Driven Revolution in Genome Editing
    Zhidong Li, Wasi Ullah Khan, Genxiang Bai, Dong Chao, Jungang Wang, Youpeng Zhang, Chong Wang, Hongbin Zhang, Wenyi Wang, Ming Luo et al. · 2025 · Advanced Science
67. CRISPR-Csy4-Mediated Editing of Rotavirus Double-Stranded RNA Genome
    Guido Papa, Luca Venditti, Luca Braga, Edoardo Schneider, Mauro Giacca, Gianluca Petris, Óscar R. Burrone · 2020 · Cell Reports
68. Current widely-used web-based tools for CRISPR nucleases, base editors, and prime editors
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69. An Overview of Genome‐Engineering Methods
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70. Engineering a New Generation of Gene Editors: Integrating Synthetic Biology and AI Innovations
    Bing Shao Chia, Samantha Seah, Bolun Wang, Kimberle Shen, Diya Srivastava, Wei Leong Chew · 2025 · ACS Synthetic Biology
71. A sensitive visual method for onsite detection of quarantine pathogenic bacteria from horticultural crops using an LbCas12a variant system
    Jian Jiao, Mengjie Yang, Tengfei Zhang, Yingli Zhang, Mengli Yang, Ming Li, Chonghuai Liu, Shangwei Song, Tuanhui Bai, Chunhui Song et al. · 2021 · Journal of Hazardous Materials
72. Fishing for understanding: Unlocking the zebrafish gene editor’s toolbox
    Brandon W. Simone, Gabriel Martínez‐Gálvez, Zachary WareJoncas, Stephen Carl Ekker · 2018 · Methods
73. CRISPR-Cas12a with an oAd Induces Precise and Cancer-Specific Genomic Reprogramming of EGFR and Efficient Tumor Regression
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74. Engineering guide RNA to reduce the off-target effects of CRISPR
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75. Machine learning-based prediction models to guide the selection of Cas9 variants for efficient gene editing
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76. Saturation profiling of drug-resistant genetic variants using prime editing
    Younggwang Kim, Hyeong-Cheol Oh, Seung‐Ho Lee, Seokjoong Kim · 2024 · Nature Biotechnology
77. C-SVR Crispr: Prediction of CRISPR/Cas12 guideRNA activity using deep learning models
    Zubaida Said Ameen, Mehmet Özsöz, Auwalu Saleh Mubarak, Fadi M. Al-Turjman, Sertan Serte · 2021 · Alexandria Engineering Journal
78. Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA
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79. How to Completely Squeeze a Fungus—Advanced Genome Mining Tools for Novel Bioactive Substances
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80. Massively parallel CRISPRi assays reveal concealed thermodynamic determinants of dCas12a binding
    David A. Specht, Yasu Xu, Guillaume Lambert · 2020 · Proceedings of the National Academy of Sciences
81. Interpreting CRISPR-Cas12a enzyme kinetics through free energy change of nucleic acids
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82. Synthetic mismatches enable specific CRISPR-Cas12a-based detection of genome-wide SNVs tracked by ARTEMIS
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83. Engineered dual selection for directed evolution of SpCas9 PAM specificity
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84. CRISPR/Cas12a-mediated labeling of MET receptor enables quantitative single-molecule imaging of endogenous protein organization and dynamics
    Tim N. Baldering, Christos Karathanasis, Marie-Lena I.E. Harwardt, Petra Freund, Matthias Meurer, Johanna V. Rahm, Michael Knop, Marina S. Dietz, Mike Heilemann · 2020 · iScience
85. Efficient genome editing in wheat using Cas9 and Cpf1 (AsCpf1 and LbCpf1) nucleases
    Dong-Jin Kim, Megan S. Hager, Eleanor J. Brant, Hikmet Budak · 2021 · Functional & Integrative Genomics
86. Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants
    Karthik Murugan, Shravanti Krishna Suresh, Arun Somwarpet Seetharam, Andrew Josef Severin, Dipali G. Sashital · 2021 · Nucleic Acids Research
87. Optimization of Nuclear Localization Signal Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells
    Kevin Luk, Pengpeng Liu, Jing Zeng, Yetao Wang, Stacy A. Maitland, Feston Idrizi, Karthikeyan Ponnienselvan, Lihua Julie Zhu, Jeremy Luban, Daniel E. Bauer et al. · 2022 · GEN Biotechnology
88. Rapid and Simple Detection of Burkholderia gladioli in Food Matrices Using RPA-CRISPR/Cas12a Method
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89. Massively parallel CRISPR off-target detection enables rapid off-target prediction model building
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90. Detection of Parasites in the Field: The Ever-Innovating CRISPR/Cas12a
    Xin Li, Zhisheng Dang, Wenqiang Tang, Haoji Zhang, Jian‐Wei Shao, Rui JIANG, Xu Zhang, Fuqiang Huang · 2024 · Biosensors
91. Genome editing in the mammalian brain using the CRISPR–Cas system
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92. Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells
    Ellen Slaman, Lisanne Kottenhagen, William de Martines, Gerco C. Angenent, Ruud A. de Maagd · 2024 · Scientific Reports
93. Engineering of Cas12a nuclease variants with enhanced genome-editing specificity
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94. Strategies for improving the genome-editing efficiency of class 2 CRISPR/Cas system
    Linli Wang, Hongbing Han · 2024 · Heliyon
95. Split technology in sensors based on CRISPR/Cas12a system
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96. Therapeutic application of the CRISPR system: current issues and new prospects
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97. CRISPR-gRNA Design
    Maria Pallarès-Masmitjà, Nastassia Knödlseder, Marc Güell · 2019 · Methods in molecular biology
98. Development and application of DETECTR-based rapid detection for pathogenic Bacillus anthracis
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99. How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis
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100. CRISPRpic: fast and precise analysis for CRISPR-induced mutations via p refixed i ndex c ounting
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