In vivo high-throughput profiling of CRISPR-Cpf1 activity

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Items 201–300 of 384

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2. Discovery of CRISPR-Cas12a clades using a large language model
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3. Efficient genome editing by CRISPR-Mb3Cas12a in mice
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5. The promise and peril of CRISPR gene drives
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6. Domain-inlaid Nme2Cas9 adenine base editors with improved activity and targeting scope
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7. Development of a Recombinase Polymerase Amplification-Coupled CRISPR/Cas12a Platform for Rapid Detection of Antimicrobial-Resistant Genes in Carbapenem-Resistant Enterobacterales
   Ji Woo Yang, Heesu Kim, Lee-Sang Hyeon, Jung-Sik Yoo, Sangrim Kang · 2024 · Biosensors
8. Massively parallel evaluation and computational prediction of the activities and specificities of 17 small Cas9s
   Sang-Yeon Seo, Seonwoo Min, Sungtae Lee, Jung Hwa Seo, Jinman Park, Hui Kwon Kim, Myungjae Song, Dawoon Baek, Sung‐Rae Cho, Seokjoong Kim · 2023 · Nature Methods
9. CRISPR-Cas and CRISPR-based screening system for precise gene editing and targeted cancer therapy
   Mingming Qin, Chunhao Deng, Liewei Wen, Guoqun Luo, Ya Meng · 2024 · Journal of Translational Medicine
10. Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles
    Prarthana Mohanraju, Ioannis Mougiakos, Justin Albers, Megumu Yamada Mabuchi, Ryan T. Fuchs, Jennifer L. Curcuru, Richard van Kranenburg, G. Brett Robb, John van der OOST · 2021 · The CRISPR Journal
11. Mutations generated by repair of Cas9-induced double strand breaks are predictable from surrounding sequence
    Felicity R. Allen, Luca Crepaldi, Clara Alsinet, Alexander J. Strong, Vitalii Kleshchevnikov, Pietro De Angeli, Petra Páleníková, Michael Kosicki, Andrew Roger Bassett, Heather P. Harding et al. · 2018 · bioRxiv (Cold Spring Harbor Laboratory)
12. Unraveling the future of genomics: CRISPR, single-cell omics, and the applications in cancer and immunology
    A Vipin Menon, Bicna Song, Lumen Chao, Diksha Sriram, Pamela A. Chansky, Ishnoor Bakshi, Jane Ulianova, Wei Li · 2025 · Frontiers in Genome Editing
13. Sophisticated CRISPR/Cas tools for fine-tuning plant performance
    Niklas Capdeville, Laura Merker, Patrick Schindele, Holger Puchta · 2020 · Journal of Plant Physiology
14. CRISPR Systems Suitable for Single AAV Vector Delivery
    Marta Stevanovic, Elena Piotter, Michelle E. McClements, Robert Edward MacLaren · 2021 · Current Gene Therapy
15. Efficient Single-Nucleotide Microbial Genome Editing Achieved Using CRISPR/Cpf1 with Maximally 3′-End-Truncated crRNAs
    Ho Joung Lee, Hyun Ju Kim, Young‐Jun Park, Sang Jun Lee · 2022 · ACS Synthetic Biology
16. Advancing CRISPR/Cas gene editing with machine learning
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17. Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells
    Lucas Kissling, Asun Monfort, Daan C. Swarts, Anton Wutz, Martin Jínek · 2019 · Methods in enzymology on CD-ROM/Methods in enzymology
18. Identification of SaCas9 orthologs containing a conserved serine residue that determines simple NNGG PAM recognition
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19. Optimization of CRISPR–Cas system for clinical cancer therapy
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20. Genome editing prospects for heat stress tolerance in cereal crops
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21. Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast
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22. Dissecting Tissue-Specific Super-Enhancers by Integrating Genome-Wide Analyses and CRISPR/Cas9 Genome Editing
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23. Dynamic basis of supercoiling-dependent DNA interrogation by Cas12a via R-loop intermediates
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24. Functional assessment of all ATM SNVs using prime editing and deep learning
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25. BoostMEC: predicting CRISPR-Cas9 cleavage efficiency through boosting models
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26. Two CRISPR/Cas12a-based methods for fast and accurate detection of single-base mutations
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27. TheFrancisella novicidaCas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays
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28. Uncertainty-aware and interpretable evaluation of Cas9–gRNA and Cas12a–gRNA specificity for fully matched and partially mismatched targets with Deep Kernel Learning
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29. Expanding the CRISPR toolbox for Chinese hamster ovary cells with comprehensive tools for Mad7 genome editing
    Johan Rojek, Yogesh Basavaraju, Saranya Nallapareddy, Dubhe B. Bulté, Roland Baumgartner, Sanne Schoffelen, Lise Marie Grav, Steffen Goletz, Lasse Ebdrup Pedersen · 2023 · Biotechnology and Bioengineering
30. A universal method for sensitive and cell-free detection of CRISPR-associated nucleases
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31. Highly parallel profiling of the activities and specificities of Cas12a variants in human cells
    Peng Chen, Yankang Wu, Hongjian Wang, Huan Liu, Jin Zhou, Jingli Chen, Jun Lei, Zaiqiao Sun, Chonil Paek, Lei Yin · 2025 · Nature Communications
32. Efficient multiplex CRISPR/Cpf1 (Cas12a) genome editing system in Aspergillus aculeatus TBRC 277
    Dede Abdulrachman S, Verawat Champreda, Lily Eurwilaichitr, Duriya Chantasingh, Kusol Pootanakit · 2022 · Journal of Biotechnology
33. Site-specific mutagenesis screening in KRASG12D mutant library to uncover resistance mechanisms to KRASG12D inhibitors
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34. CRISPR-Directed In Vitro Gene Editing of Plasmid DNA Catalyzed by Cpf1 (Cas12a) Nuclease and a Mammalian Cell-Free Extract
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35. Multiplexed activation in mammalian cells using a split-intein CRISPR/Cas12a based synthetic transcription factor
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36. Expanded targeting scope of LbCas12a variants allows editing of multiple oncogenic mutations
    Eunyoung Choi, Hye-Yeon Hwang, Eun‐Ji Kwon, Daesik Kim, Taeyoung Koo · 2022 · Molecular Therapy — Nucleic Acids
37. Visual and self-contained diagnosis of Salmonella using RPA-CRISPR on a centrifugal force driven microfluidic system
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38. Split dCas12a activator for lncRNA H19 activation to enhance BMSC differentiation and promote calvarial bone healing
    Nuong Thi Kieu Nguyen, Yi Tu, Hsiang-Sheng Lee, Vu Anh Truong, Yi‐Hao Chang, Nam Ngoc Pham, Chin-Wei Chang, Ya-Hui Lin, Po-Liang Lai, Pin‐Hsin Chen et al. · 2023 · Biomaterials
39. Eukaryotic-driven directed evolution of Cas9 nucleases
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40. Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope
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41. Selict-seq profiles genome-wide off-target effects in adenosine base editing
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42. Decoding CRISPR–Cas PAM recognition with UniDesign
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43. Carboxylic acid reductase-dependent biosynthesis of eugenol and related allylphenols
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44. The application of CRISPR-Cas system in Staphylococcus aureus infection
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45. CRISPR-Cas12a ribonucleoprotein-mediated gene editing in the plant pathogenic fungus Magnaporthe oryzae
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46. Expansion of the CRISPR/Cas Genome-Sculpting Toolbox: Innovations, Applications and Challenges
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47. Identification of thermotolerant non-canonical PAMs for robust one-pot CRISPR-Cas12a detection
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48. Multiplex genome engineering in Clostridium beijerinckii NCIMB 8052 using CRISPR-Cas12a
    Constantinos Patinios, Stijn T. de Vries, Mamou Diallo, Lucrezia Lanza, Pepijn L. J. V. Q. Verbrugge, Ana María López-Contreras, John van der OOST, Ruud A. Weusthuis, Servé W. M. Kengen · 2023 · Scientific Reports
49. Comparison of CRISPR/Cas9 and Cas12a for gene editing in Chlamydomonas reinhardtii
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50. The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs
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51. Modeling Non-Alcoholic Fatty Liver Disease (NAFLD) Using “Good-Fit” Genome-Editing Tools
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52. Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough
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53. Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering
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54. CGD: Comprehensive guide designer for CRISPR-Cas systems
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55. Single‐Cell Immunoblotting based on a Photoclick Hydrogel Enables High‐Throughput Screening and Accurate Profiling of Exogenous Gene Expression
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56. Nucleases in gene-editing technologies: past and prologue
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57. The Evolution of Nucleic Acid–Based Diagnosis Methods from the (pre-)CRISPR to CRISPR era and the Associated Machine/Deep Learning Approaches in Relevant RNA Design
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58. Engineering an Escherichia coli strain for enhanced production of flavonoids derived from pinocembrin
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59. High-Throughput Profiling of Cas12a Orthologues and Engineered Variants for Enhanced Genome Editing Activity
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60. Sequence modification on demand: search and replace tools for precise gene editing in plants
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61. Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA
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62. Predicting adenine base editing efficiencies in different cellular contexts by deep learning
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63. Evaluation and prediction of guide RNA activities in genome-editing tools
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64. A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells
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65. CRISPR/Cas9 mediated genome editing tools and their possible role in disease resistance mechanism
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66. In vivo gene correction with targeted sequence substitution through microhomology-mediated end joining
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67. Rapid and Specific Detection of Active SARS-CoV-2 With CRISPR/Cas12a
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68. Genome Editing in Forest Trees
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69. Genome editing of algal species by CRISPR Cas9 for biofuels
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70. Prime editing enables precise genome editing in mouse liver and retina
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71. Precise CRISPR/Cpf1 genome editing system in the Deinococcus radiodurans with superior DNA repair mechanisms
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72. Simultaneous Knockout of Tk1‐SST and Tk1‐FFT via CRISPR /Cas9 Enhances the Natural Rubber Accumulation in Taraxacum kok‐saghyz
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73. Recognition of CRISPR Off-Target Cleavage Sites with SeqGAN
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74. Optimizing ErCas12a for efficient gene editing in Arabidopsis thaliana
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75. Genome Editing: Advances and Prospects
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76. CRISPR-Cas12a biosensing technology advances and applications in precision diagnostics and cancer research
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77. Strategies and mechanisms of precision genome engineering: From gene editing to genome writing
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78. gDesigner: computational design of synthetic gRNAs for Cas12a-based transcriptional repression in mammalian cells
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79. Establishment of a Rapid Detection Technique Based on RPA-LFD and RPA-CRISPR/Cas12a on Phytophthora pini
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81. Three novel Cas12a orthologs with robust DNA cleavage activity suitable for nucleic acid detection
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82. Establishment of a rapid detection system for Phytophthora syringae based on RPA/CRISPR-cas12a
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83. Key thermodynamic characteristics of Cas9 and Cas12a endonucleases’ cleavage of a DNA substrate containing a nucleotide mismatch in the region complementary to RNA
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84. Molecular mechanisms and biotechnology applications of CRISPR–Cas12a
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87. Analysis of Wild Type LbCpf1 Protein, and PAM Recognition Variants, in a Cellular Context
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88. New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
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90. Multiplexed Genome Engineering with Cas12a
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91. A Highly Sensitive GFP Activation Assay for Detection of DNA Cleavage in Cells
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92. An Introduced RNA-Only Approach for Plasmid Curing via the CRISPR-Cpf1 System in Saccharomyces cerevisiae
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97. Genome Editing to Develop Disease Resistance in Crops
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99. Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a
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100. Enhanced production of functional CRISPR-AsCas12a protein in Escherichia coli
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