In vivo high-throughput profiling of CRISPR-Cpf1 activity

Kim, Hui K, Song, Myungjae, Lee, Jinu, Menon, A Vipin, Jung, Soobin, Kang, Young-Mook, Choi, Jae W, Woo, Euijeon +3 more·2017·Nature Methods
DOI 10.1038/nmeth.4104

Cited by (384)

Items 301–384 of 384

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  11. Homology-Directed Transgene-Free Gene Editing in Chlamydomonas reinhardtii
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  13. Method Development and Sensitivity Optimization Paradigm for One-Step RPA-CRISPR/Cas12a Rapid Detection of the Hard-to-Amplify Gene bla NDM
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  14. CRISPR/Cas12a-based biosensors for the detection of pathogenic bacteria in dairy products: A comprehensive review
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  15. SDS‐CRISPR for Single‐Nucleotide Variant Detection
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  16. Precise Multi‐Gene Editing Strategies for Xenotransplantation Donor Pigs: Overcoming Immune and Coagulation Barriers
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  20. Inducible, tunable and multiplex human gene regulation using CRISPR-Cpf1-based transcription factors
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  21. SpCas9 activity prediction by DeepSpCas9, a deep learning-based model with unparalleled generalization performance
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  22. Edition of complex gene families in tobacco with GoldenBraid 4.0, a multipurpose web-based platform for plant genome engineering
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  24. Structure-function relations of RNA molecules involved in gene expression and host defence
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  25. Saturation resistance profiling of EGFR variants against tyrosine kinase inhibitors using prime editing
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  26. Expanding the CRISPR toolbox by engineering Cas12a orthologs of metagenomic discovery
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  30. Development of a field-deployable RPA-CRISPR/Cas12a assay for the detection of Cyclospora cayetanensis in human feces
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  31. CRISPR RNA Engineering Enables Single Nucleotide Polymorphism Discrimination in Nucleic Acid Detection
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  32. CRISPR RNA Engineering Enables Single Nucleotide Polymorphism Discrimination in Nucleic Acid Detection
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  33. An improved CNN-LSTM deep model for Classification of guideRNA in CRISPR-Casl2 System
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  34. Genome Editing and Fatty Liver
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  35. A quantitative assay for the efficiency of RNA ‐guided genome editing in plants
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  36. CNN-SVR for CRISPR-Cpf1 Guide RNA Activity Prediction with Data Augmentation
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  37. Can Deep Learning Solve the Cas9 Dilemma?
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  38. Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens
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  39. Optimization of NLS Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells
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  40. Hyongbum (Henry) Kim
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  41. Optimizing Heterologous Production of CRISPR-AsCas12a Protein in Escherichia coli
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  42. DeepEmbCas9: Cas9 coevolution and sgRNA structural information for CRISPR-Cas9 cleavage activity prediction
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  43. Systematically investigating the key features of the nuclease deactivated Cpf1 for tunable multiplex genetic regulation
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  44. Efficient multiplex genome editing using CRISPR-Mb3Cas12a in mice
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  45. In vivo gene editing of outer hair cells prevents progressive hearing loss in a dominant-negative KCNQ4 murine model
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  46. CRISPR-VAE: A Method for Explaining CRISPR/Cas12a Predictions, and an Efficiency-aware gRNA Sequence Generator
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  47. Prediction of activity of CRISPR-Cpf1 guide RNA via in vivo high-throughput screening
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  48. Improving CRISPR tools by elucidating DNA repair
    Jung Min Lim, Seokjoong Kim · 2021 · Nature Biotechnology
  49. Faculty Opinions recommendation of Switching the activity of Cas12a using guide RNA strand displacement circuits.
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  50. Karyotype diversification in colorectal cancer
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  51. Computational Tools for Target Design and Analysis
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  53. Engineering Viral Vectors for CRISPR-Cas Mediated Genome Editing in Plants
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  54. Multiplex genome engineering in Clostridium beijerinckii NCIMB 8052 using CRISPR-Cas12a
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  57. Metode CRISPR/CAS dan Minimalisasi Off-Target: Review
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  58. Additional file 3 of A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing
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  59. High‐fidelity CRISPR/Cas12a dual‐crRNA screening reveals novel synergistic interactions in hepatocellular carcinoma
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  62. Additional file 1 of Identification of pathogenic variants in cancer genes using base editing screens with editing efficiency correction
    Changcai Huang, Li, Guangyu, Wu, Jiayu, Liang, Junbo, Wang, Xiaoyue · 2021 · Figshare
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  66. Additional file 2 of BoostMEC: predicting CRISPR-Cas9 cleavage efficiency through boosting models
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  67. Additional file 1 of Eukaryotic-driven directed evolution of Cas9 nucleases
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  68. Additional file 2 of Eukaryotic-driven directed evolution of Cas9 nucleases
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  69. Additional file 3 of Eukaryotic-driven directed evolution of Cas9 nucleases
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  73. Additional file 1 of BoostMEC: predicting CRISPR-Cas9 cleavage efficiency through boosting models
    Oscar A. Zarate, Yang, Yiben, Wang, Xiaozhong, Wang, Ji-Ping · 2022 · Figshare
  74. Additional file 1 of A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing
    Chen, Peng, Zhou, Jin, Yibin Wan, Liu, Huan, Li, Yongzheng, Liu, Zhaoxin, Wang, Hongjian, Lei, Jun, Zhao, Kai, Zhang, Yiliang et al. · 2020 · Figshare
  75. Additional file 13 of Identification of pathogenic variants in cancer genes using base editing screens with editing efficiency correction
    Changcai Huang, Li, Guangyu, Wu, Jiayu, Liang, Junbo, Wang, Xiaoyue · 2021 · Figshare
  76. Development of genetic control strategies for insect pests using CRISPR/Cas9
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  77. Parallel Activation and Interference CRISPR (PAIR) with Sequencing Uncovers DNA Repair Networks Guiding Precision Cell Engineering
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