In vivo high-throughput profiling of CRISPR-Cpf1 activity

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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

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Predicting the mutations generated by repair of Cas9-induced double-strand breaks

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A highly specific SpCas9 variant is identified by in vivo screening in yeast

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Engineered Cpf1 variants with altered PAM specificities

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Base editing with a Cpf1–cytidine deaminase fusion

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Deep learning improves prediction of CRISPR–Cpf1 guide RNA activity

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Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning

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Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1

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CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

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PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures

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Predicting the efficiency of prime editing guide RNAs in human cells

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sgRNA Scorer 2.0: A Species-Independent Model To Predict CRISPR/Cas9 Activity

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Cas9 versus Cas12a/Cpf1: Structure–function comparisons and implications for genome editing

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Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis

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dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression

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SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with high generalization performance

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Activities and specificities of CRISPR /Cas9 and Cas12a nucleases for targeted mutagenesis in maize

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Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

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Pooled In Vitro and In Vivo CRISPR-Cas9 Screening Identifies Tumor Suppressors in Human Colon Organoids

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Optimization of AsCas12a for combinatorial genetic screens in human cells

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Design and analysis of CRISPR–Cas experiments

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Prediction of the sequence-specific cleavage activity of Cas9 variants

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Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a

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Marker-free coselection for CRISPR-driven genome editing in human cells

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Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases

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Evaluating and enhancing target specificity of gene-editing nucleases and deaminases

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Predicting prime editing efficiency and product purity by deep learning

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Delivery approaches for CRISPR/Cas9 therapeutics in vivo: advances and challenges

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MeCas12a, a Highly Sensitive and Specific System for COVID‐19 Detection

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Assessment of Cas12a‐mediated gene editing efficiency in plants

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CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects

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Genome-wide profiling of adenine base editor specificity by EndoV-seq

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Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases

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Immunity to CRISPR Cas9 and Cas12a therapeutics

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High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells

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Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review)

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FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae

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Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9–Cas12a platform

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Switching the activity of Cas12a using guide RNA strand displacement circuits

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CRISPR-Cas-mediated transcriptional modulation: The therapeutic promises of CRISPRa and CRISPRi

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The chemistry of Cas9 and its CRISPR colleagues

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Massively parallel kinetic profiling of natural and engineered CRISPR nucleases

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Exploring the Trans‐Cleavage Activity of CRISPR‐Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

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Prediction of efficiencies for diverse prime editing systems in multiple cell types

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Sequence-specific prediction of the efficiencies of adenine and cytosine base editors

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Genome editing for plant research and crop improvement

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Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice

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Enhanced Cas12a editing in mammalian cells and zebrafish

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Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing

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Structural basis for the altered PAM recognition by engineered CRISPR-Cpf1

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Good guide, bad guide: spacer sequence-dependent cleavage efficiency of Cas12a

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Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

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Transforming plant biology and breeding with CRISPR /Cas9, Cas12 and Cas13

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The Rise of the CRISPR/Cpf1 System for Efficient Genome Editing in Plants

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A split CRISPR–Cpf1 platform for inducible genome editing and gene activation

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Targeting mutant <i>KRAS</i> with CRISPR-Cas9 controls tumor growth

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Predicting base editing outcomes with an attention-based deep learning algorithm trained on high-throughput target library screens

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CRISPR-LbCpf1 prevents choroidal neovascularization in a mouse model of age-related macular degeneration

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CRISPR/Cas9-based epigenome editing: An overview of dCas9-based tools with special emphasis on off-target activity

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CRISPR-DT: designing gRNAs for the CRISPR-Cpf1 system with improved target efficiency and specificity

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Discovery of Diverse CRISPR-Cas Systems and Expansion of the Genome Engineering Toolbox

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Cas9, Cpf1 and C2c1/2/3―What's next?

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Novel Epigenetic Techniques Provided by the CRISPR/Cas9 System

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Generalizable sgRNA design for improved CRISPR/Cas9 editing efficiency

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The application of DNA polymerases and Cas9 as representative of DNA-modifying enzymes group in DNA sensor design (review)

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A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing

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CRISPR–Cas9 Gene Editing: Curing Genetic Diseases by Inherited Epigenetic Modifications

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Efficient Transcriptional Gene Repression by Type V-A CRISPR-Cpf1 from Eubacterium eligens

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Recent Advances in CRISPR-Cas Technologies for Synthetic Biology

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CRISPR/Cas-Based Modifications for Therapeutic Applications: A Review

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Deep learning models to predict the editing efficiencies and outcomes of diverse base editors

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Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a

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Cas12a domain flexibility guides R-loop formation and forces RuvC resetting

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Revolutionizing CRISPR technology with artificial intelligence

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CRISPR-Cas12a–assisted PCR tagging of mammalian genes

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Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants

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Making the cut(s): how Cas12a cleaves target and non-target DNA

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Genome editing technology and application in soybean improvement

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Multiplexed orthogonal genome editing and transcriptional activation by Cas12a

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High-throughput functional evaluation of human cancer-associated mutations using base editors

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Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors

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Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection

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CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors

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In vivo profiling of metastatic double knockouts through CRISPR–Cpf1 screens

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Sniper2L is a high-fidelity Cas9 variant with high activity

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In vivo outer hair cell gene editing ameliorates progressive hearing loss in dominant-negative Kcnq4 murine model

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Genome Editing using CRISPR-Cas9 and -Cpf1

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Design and assessment of engineered CRISPR–Cpf1 and its use for genome editing

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Prediction of activity of CRISPR-Cpf1 guide RNA via in vivo high-throughput screeningPreprint

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Prediction of activity and specificity of CRISPR-Cpf1 using convolutional deep learning neural networks

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A Survey of Genome Editing Activity for 16 Cpf1 orthologsPreprint

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