The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Iterative expansion microscopy
Open accessAbstract
We recently developed a method called expansion microscopy, in which preserved biological specimens are physically magnified by embedding them in a densely crosslinked polyelectrolyte gel, anchoring key labels or biomolecules to the gel, mechanically homogenizing the specimen, and then swelling the gel–specimen composite by ∼4.5× in linear dimension. Here we describe iterative expansion microscopy (iExM), in which a sample is expanded ∼20×. After preliminary expansion a second swellable polymer mesh is formed in the space newly opened up by the first expansion, and the sample is expanded again. iExM expands biological specimens ∼4.5 × 4.5, or ∼20×, and enables ∼25-nm-resolution imaging of cells and tissues on conventional microscopes. We used iExM to visualize synaptic proteins, as well as the detailed architecture of dendritic spines, in mouse brain circuitry.
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Cites
Spatially resolved, highly multiplexed RNA profiling in single cells
Chen, K. H., Boettiger, A. N., Moffitt, J. R., Wang, S., & Zhuang, X. (2015) · Science · doi:10.1126/science.aaa6090 · Cited by 2,969
Independent optical excitation of distinct neural populations
Klapoetke, N. C., Murata, Y., Kim, S. S., Pulver, S. R., Birdsey-Benson, A., Cho, Y. K., Morimoto, T. K., Chuong, A. S., Carpenter, E. J., Tian, Z., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2836 · Cited by 2,344
Self-citationLattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
Chen, B.-C., Legant, W. R., Wang, K., Shao, L., Milkie, D. E., Davidson, M. W., Janetopoulos, C., Wu, X. S., Hammer, J. A., Liu, Z., et al. (2014) · Science · doi:10.1126/science.1257998 · Cited by 1,919
Chen, F., Tillberg, P. W., & Boyden, E. S. (2015) · Science · doi:10.1126/science.1260088 · Cited by 1,501
Self-citationEvaluation of fluorophores for optimal performance in localization-based super-resolution imaging
Dempsey, G. T., Vaughan, J. C., Chen, K. H., Bates, M., & Zhuang, X. (2011) · Nature Methods · doi:10.1038/nmeth.1768 · Cited by 1,369
High-performance genetically targetable optical neural silencing by light-driven proton pumps
Chow, B. Y., Han, X., Dobry, A. S., Qian, X., Chuong, A. S., Li, M., Henninger, M. A., Belfort, G. M., Lin, Y., Monahan, P. E., et al. (2010) · Nature · doi:10.1038/nature08652 · Cited by 1,186
Self-citationMultiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT
Jungmann, R., Avendaño, M. S., Woehrstein, J. B., Dai, M., Shih, W. M., & Yin, P. (2014) · Nature Methods · doi:10.1038/nmeth.2835 · Cited by 1,128
Single-cell in situ RNA profiling by sequential hybridization
Lubeck, E., Coskun, A. F., Zhiyentayev, T., Ahmad, M., & Cai, L. (2014) · Nature Methods · doi:10.1038/nmeth.2892 · Cited by 985
Highly Multiplexed Subcellular RNA Sequencing in Situ
Lee, J. H., Daugharthy, E. R., Scheiman, J., Kalhor, R., Yang, J. L., Ferrante, T. C., Terry, R., Jeanty, S. S. F., Li, C., Amamoto, R., et al. (2014) · Science · doi:10.1126/science.1250212 · Cited by 973
SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction
Ke, M.-T., Fujimoto, S., & Imai, T. (2013) · Nature Neuroscience · doi:10.1038/nn.3447 · Cited by 892
Dell, R. B., Holleran, S., & Ramakrishnan, R. (2002) · ILAR Journal · doi:10.1093/ilar.43.4.207 · Cited by 741
Superresolution Imaging of Chemical Synapses in the Brain
Dani, A., Huang, B., Bergan, J., Dulac, C., & Zhuang, X. (2010) · Neuron · doi:10.1016/j.neuron.2010.11.021 · Cited by 725
Programmable in situ amplification for multiplexed imaging of mRNA expression
Choi, H. M. T., Chang, J. Y., Trinh, L. A., Padilla, J. E., Fraser, S. E., & Pierce, N. A. (2010) · Nature Biotechnology · doi:10.1038/nbt.1692 · Cited by 715
Self-citationTillberg, P. W., Chen, F., Piatkevich, K. D., Zhao, Y., Yu, C.-C., English, B. P., Gao, L., Martorell, A., Suk, H.-J., Yoshida, F., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3625 · Cited by 664
Self-citationKu, T., Swaney, J., Park, J.-Y., Albanese, A., Murray, E., Cho, J. H., Park, Y.-G., Mangena, V., Chen, J., & Chung, K. (2016) · Nature Biotechnology · doi:10.1038/nbt.3641 · Cited by 458
Expansion microscopy with conventional antibodies and fluorescent proteins
Chozinski, T. J., Halpern, A. R., Okawa, H., Kim, H.-J., Tremel, G. J., Wong, R. O. L., & Vaughan, J. C. (2016) · Nature Methods · doi:10.1038/nmeth.3833 · Cited by 447
Improved tools for the Brainbow toolbox
Cai, D., Cohen, K. B., Luo, T., Lichtman, J. W., & Sanes, J. R. (2013) · Nature Methods · doi:10.1038/nmeth.2450 · Cited by 426
Self-citationNanoscale imaging of RNA with expansion microscopy
Chen, F., Wassie, A. T., Cote, A. J., Sinha, A., Alon, S., Asano, S., Daugharthy, E. R., Chang, J.-B., Marblestone, A., Church, G. M., et al. (2016) · Nature Methods · doi:10.1038/nmeth.3899 · Cited by 404
Self-citationHigh-density three-dimensional localization microscopy across large volumes
Legant, W. R., Shao, L., Grimm, J. B., Brown, T. A., Milkie, D. E., Avants, B. B., Lavis, L. D., & Betzig, E. (2016) · Nature Methods · doi:10.1038/nmeth.3797 · Cited by 288
Weber, K., Rathke, P. C., & Osborn, M. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.4.1820 · Cited by 255
Aquino, D., Schönle, A., Geisler, C., Middendorff, C. V., Wurm, C. A., Okamura, Y., Lang, T., Hell, S. W., & Egner, A. (2011) · Nature Methods · doi:10.1038/nmeth.1583 · Cited by 254
Superabsorbent Hydrogels That Are Robust and Highly Stretchable
Cipriano, B. H., Banik, S. J., Sharma, R., Rumore, D., Hwang, W., Briber, R. M., & Raghavan, S. R. (2014) · Macromolecules · doi:10.1021/ma500882n · Cited by 213
Resolution Doubling in 3D-STORM Imaging through Improved Buffers
Olivier, N., Keller, D., Gönczy, P., & Manley, S. (2013) · PLoS ONE · doi:10.1371/journal.pone.0069004 · Cited by 188
Chemical tags: Applications in live cell fluorescence imaging
Wombacher, R., & Cornish, V. W. (2011) · Journal of Biophotonics · doi:10.1002/jbio.201100018 · Cited by 93
Alkaline Hydrolysis of Polyacrylamide
Kurenkov, V. F., Hartan, H. G., & Lobanov, F. I. (2001) · Russian Journal of Applied Chemistry · doi:10.1023/a:1012786826774 · Cited by 74
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Saka, S. K., Wang, Y., Kishi, J. Y., Zhu, A., Zeng, Y., Xie, W., Kirli, K., Yapp, C., Cicconet, M., Beliveau, B. J., et al. (2018) · doi:10.1101/507566 · Cited by 485
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Saka, S. K., Wang, Y., Kishi, J. Y., Zhu, A., Zeng, Y., Xie, W., Kirli, K., Yapp, C., Cicconet, M., Beliveau, B. J., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0207-y · Cited by 481
Self-citationMurakami, T. C., Mano, T., Saikawa, S., Horiguchi, S. A., Shigeta, D., Baba, K., Sekiya, H., Shimizu, Y., Tanaka, K. F., Kiyonari, H., et al. (2018) · Nature Neuroscience · doi:10.1038/s41593-018-0109-1 · Cited by 292
One-step nanoscale expansion microscopy reveals individual protein shapes
Shaib, A. H., Chouaib, A. A., Chowdhury, R., Altendorf, J., Mihaylov, D., Zhang, C., Krah, D., Imani, V., Spencer, R. K. W., Georgiev, S. V., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-024-02431-9 · Cited by 104
Self-citation
Metadata
- Authors
- Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock, H., Kang, J. S., Asano, S., Suk, H.-J., Pak, N., Tillberg, P. W., Wassie, A. T., Cai, D., & Boyden, E. S.
- Venue
- Nature Methods
- Published
- 2017-04-17
- Volume
- 14
- Issue
- 6
- Pages
- 593-599
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W4232515155
- Cited by
- 386
- References
- 25
- FWCI
- 19.48
- Type
- journal article
- Language
- en
- Open access
- green
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